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Literature summary extracted from

  • Choi, Y.S.; Zhang, H.; Brunzelle, J.S.; Nair, S.K.; Zhao, H.
    In vitro reconstitution and crystal structure of p-aminobenzoate N-oxygenase (AurF) involved in aureothin biosynthesis (2008), Proc. Natl. Acad. Sci. USA, 105, 6858-6863 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.99.68 ascorbate the in vitro enzymatic activity of AurF is best reconstituted in the presence of phenazine methosulfate and ascorbate Streptomyces thioluteus
1.14.99.68 phenazine methosulfate the in vitro enzymatic activity of AurF is best reconstituted in the presence of phenazine methosulfate and ascorbate Streptomyces thioluteus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.68 structure of AurF in the oxidized state, and the cocrystal structure of AurF with its product 4-nitrobenzoate Streptomyces thioluteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.68 0.0052
-
 4-Aminobenzoate pH 7.4, 20°C Streptomyces thioluteus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.68 Iron the iron-to-enzyme ratio is 2.2, protein shows a high selectivity for iron Streptomyces thioluteus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.68 Streptomyces thioluteus Q70KH9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.68 4-aminobenzoate + reduced acceptor + 2 O2
-
 Streptomyces thioluteus 4-nitrobenzoate + acceptor + 2 H2O
-
 ?
1.14.99.68 additional information the yield of the final product 4-nitrobenzoate is 37.2% when equimoles of reductants phenazine methosulfate and 4-aminobenzoic acid and 4 equimoles of ascorbate are used Streptomyces thioluteus ?
-
-

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.99.68 0.104
-
 4-Aminobenzoate pH 7.4, 20°C Streptomyces thioluteus

General Information

EC Number General Information Comment Organism
1.14.99.68 physiological function AurF is a non-heme di-iron monooxygenase that catalyzes sequential oxidation of aminoarenes to nitroarenes via hydroxylamine and nitroso intermediates Streptomyces thioluteus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.99.68 0.02
-
 4-Aminobenzoate pH 7.4, 20°C Streptomyces thioluteus