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Literature summary extracted from

  • Yu, X.L.; Wang, H.Y.; Leung, D.W.M.; He, Z.D.; Zhang, J.J.; Peng, X.X.; Liu, E.E.
    Overexpression of OsIAAGLU reveals a role for IAA-glucose conjugation in modulating rice plant architecture (2019), Plant Cell Rep., 38, 731-739 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.121 gene OsIAAGLU, sequence comparisons, quantitative real-time PCR enzyme expression analysis, recombinant expression of GFP-tagged wild-type and mutant enzymes in Oryza sativa plants using the Agrobacterium tumefaciens strain EHA105 transfection method Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.121 additional information overexpression of OsIAAGLU reveals a role for indole-3-acetic acid (IAA)-glucose conjugation in modulating rice plant architecture. Construction of iaaglu mutants using the CRISPR/Cas9 system. Construction of hybrids Dongjin as well as IAAGLUOE3 containing pDR5:GUS obtained by crossing pDR5:GUS plants as the male parent with wild-type, OsIAAGLU overexpression plants (IAAGLUOE3) as the female parent, respectively. OsIAAGLU-overexpressing plants are insensitive to exogenous IAA/NAA (1-naphthalene acetic acid) and exhibit reduced root gravitropism. Transgenic plant phenotypes, overview Oryza sativa Japonica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.121 cytosol transient expression of OsIAAGLU-GFP or GFP-OsIAAGLU in rice protoplasts is limited to the cytoplasm Oryza sativa Japonica Group 5829
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.121 UDP-glucose + (indol-3-yl)acetate Oryza sativa Japonica Group
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 UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.121 Oryza sativa Japonica Group A0A0P0W2E0
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.121 leaf
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 Oryza sativa Japonica Group
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2.4.1.121 seedling
-
 Oryza sativa Japonica Group
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.121 UDP-glucose + (indol-3-yl)acetate
-
 Oryza sativa Japonica Group UDP + 1-O-(indol-3-yl)acetyl-beta-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.121 indole-3-acetic acid glucosyltransferase
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 Oryza sativa Japonica Group
2.4.1.121 Os03g0693600 locus name Oryza sativa Japonica Group
2.4.1.121 OsIAAGLU
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 Oryza sativa Japonica Group

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.121 30
-
 assay at Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.121 6.5 7 assay at Oryza sativa Japonica Group

Expression

EC Number Organism Comment Expression
2.4.1.121 Oryza sativa Japonica Group expression of OsIAAGLU can be induced by indole-3-acetic acid (IAA) and 1-naphthalene acetic acid (NAA). Quantitative RT-PCR analysis show that within 2-6 h of exogenous auxin (0.01 mM IAA or 0.001 mM NAA) treatment, the expression of OsIAAGLU is upregulated in the leaves of 14-day-old rice seedlings. The response to NAA is earlier and many times greater than IAA. The transgenic plants are shorter than the wild-type plants and exhibit exaggerated leaf angles at the 5-leaf stage compared to the wild-type plants up

General Information

EC Number General Information Comment Organism
2.4.1.121 malfunction Overexpression of OsIAAGLU results in altered plant architecture. The number of tillers and leaf angle is significantly increased with a concomitant decrease in plant height and panicle length in the transgenic rice lines overexpressing OsIAAGLU compared to the wild-type plants. Phenotypes of iaaglu mutants show no obvious differences with wild-type plants. Overexpression of OsIAAGLU results in reduced sensitivity to indole-3-acetic acid (IAA) and 1-naphthalene acetic acid (NAA) and altered gravitropic response of the roots in the transgenic plants. Free IAA contents in the leaves, root tips, and lamina joint of OsIAAGLU-overexpressing transgenic lines are lower than those of wild-type plants. The transgenic plants are shorter than the wild-type plants and exhibit exaggerated leaf angles at the 5-leaf stage compared to the wild-type plants Oryza sativa Japonica Group
2.4.1.121 physiological function indole-3-acetic acid glucosyltransferase (OsIAAGLU) catalyzes the reaction of free indole-3-acetic acid (IAA) with glucose to generate IAA-glucose. OsIAAGLU plays a regulatory role in IAA homeostasis and rice architecture Oryza sativa Japonica Group