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Literature summary extracted from

  • Uraguchi, S.; Tanaka, N.; Hofmann, C.; Abiko, K.; Ohkama-Ohtsu, N.; Weber, M.; Kamiya, T.; Sone, Y.; Nakamura, R.; Takanezawa, Y.; Kiyono, M.; Fujiwara, T.; Clemens, S.
    Phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains (2017), Plant Cell Physiol., 58, 1730-1742 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.2.15 gene PCS1, isolation of four different transcript variants of OsPCS1, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient Oryza sativa Japonica Group
2.3.2.15 gene PCS1, isolation of four different transcript variants of OsPCS1, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants with OsPCS1full possessing good PCS activity in response to As(III) and Cd while the activity of the other PCS variants is very low Oryza sativa Japonica Group
2.3.2.15 gene PCS1, isolation of four different transcript variants of OsPCS1, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants with OsPCS1full possessing good PCS activity in response to As(III) and Cd while the activity of the other PCS variants is very low. Transcript of the introduced OsPCS genes is evident in all cad1-3/OsPCS lines except for cad1-3/OsPCS1b line Oryza sativa Japonica Group
2.3.2.15 gene PCS1, isolation of four different transcript variants of OsPCS1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis revealing that expression of the longest OsPCS1 variant, OsPCS1full, is most abundant, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants with OsPCS1full possessing good PCS activity in response to As(III) and Cd while the activity of the other PCS variants is very low. Phenotypes, overview Oryza sativa Japonica Group
2.3.2.15 gene PCS2, isolation of a single transcript form of OsPCS2, and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis, functional recombinant in expression in PCS-deficient mutants of Schizosaccharomyces pombe PCS knockout strain DELTApcs and Arabidopsis thaliana null mutant cad1-3 plants, the activity of PCS2 in response to As(III) and Cd is very low Oryza sativa Japonica Group

Protein Variants

EC Number Protein Variants Comment Organism
2.3.2.15 additional information identification of two independent OsPCS1 mutant rice lines, a T-DNA and a Tos17 insertion line. Thee mutant rice plants grown in soil with environmentally relevant As and Cd concentrations show increased As accumulation and decreased Cd accumulation in grains of the T-DNA line. The Tos17 mutant also exhibits the reduced Cd accumulation phenotype. These contrasting effects on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd Oryza sativa Japonica Group
2.3.2.15 additional information identification of two independent OsPCS1 mutant rice lines, a T-DNA and a Tos17 insertion line. The mutant rice plants grown in soil with environmentally relevant As and Cd concentrations show increased As accumulation and decreased Cd accumulation in grains of the T-DNA line. The Tos17 mutant also exhibits the reduced Cd accumulation phenotype. These contrasting effects on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.15 Oryza sativa Japonica Group A0A1Q2SVY7 variant OsPCS1full
-
2.3.2.15 Oryza sativa Japonica Group Q0DI55
-
-
2.3.2.15 Oryza sativa Japonica Group B7EJX7 variant OsPCS1a
-
2.3.2.15 Oryza sativa Japonica Group Q5VRI1 variant OsPCS1b
-
2.3.2.15 Oryza sativa Japonica Group A0A224ASC9 variant OsPCS1c
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.15 seed
-
 Oryza sativa Japonica Group
-
2.3.2.15 seedling
-
 Oryza sativa Japonica Group
-

Subunits

EC Number Subunits Comment Organism
2.3.2.15 additional information the OsPCS1full variant shows an AtPCS1/2-like structure with two domains Oryza sativa Japonica Group
2.3.2.15 additional information the OsPCS2 structure lacks the C-terminal domain Oryza sativa Japonica Group
2.3.2.15 additional information the OsPCS1a variant consists of only the N-terminal domain Oryza sativa Japonica Group
2.3.2.15 additional information the OsPCS1b variant consists of only the C-terminal domain Oryza sativa Japonica Group
2.3.2.15 additional information the OsPCS1c variant consists only of a very short fragment of the catalytic N-terminal domain Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
2.3.2.15 OsPCS1a
-
 Oryza sativa Japonica Group
2.3.2.15 OsPCS1b
-
 Oryza sativa Japonica Group
2.3.2.15 OsPCS1c
-
 Oryza sativa Japonica Group
2.3.2.15 OsPCS1full
-
 Oryza sativa Japonica Group
2.3.2.15 OsPCS2
-
 Oryza sativa Japonica Group
2.3.2.15 PCS
-
 Oryza sativa Japonica Group
2.3.2.15 phytochelatin synthase
-
 Oryza sativa Japonica Group
2.3.2.15 phytochelatin synthase 1
-
 Oryza sativa Japonica Group
2.3.2.15 phytochelatin synthase 2
-
 Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
2.3.2.15 malfunction the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress. Distribution of As and Cd is altered in shoot and grains of OsPCS1 mutants Oryza sativa Japonica Group
2.3.2.15 malfunction the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not Oryza sativa Japonica Group
2.3.2.15 malfunction the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS1 variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress Oryza sativa Japonica Group
2.3.2.15 malfunction the As(III)-sensitive phenotype of cad1-3 seedlings is completely rescued by the introduction of OsPCS1full variant, whereas growth of the plants expressing the other OsPCS variants is inhibited by As(III) as strongly as that of cad1-3. Similarly, OsPCS1full introduction complements the Cd-sensitive phenotype of cad1-3, while the other OsPCS1 variants do not. OsPCS1 mutant plants show increased sensitivity to Cd and As(III) stress Oryza sativa Japonica Group
2.3.2.15 metabolism contrasting effects of enzyme mutation on As and Cd distribution to grains suggest the existence of at least partially distinct PC-dependent pathways for As and Cd Oryza sativa Japonica Group
2.3.2.15 physiological function phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains. Physiological role of Oryza sativa phytochelatin synthase 1full (OsPCS1full) in the distribution and detoxification of arsenic (As) and cadmium (Cd), importance of OsPCS1-dependent PC synthesis for rice As(III) and Cd tolerance Oryza sativa Japonica Group
2.3.2.15 physiological function phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains Oryza sativa Japonica Group
2.3.2.15 physiological function phytochelatin synthase has contrasting effects on cadmium and arsenic accumulation in rice grains. Physiological role of Oryza sativa phytochelatin synthase 1 (OsPCS1) in the distribution and detoxification of arsenic (As) and cadmium (Cd), importance of OsPCS1-dependent PC synthesis for rice As(III) and Cd tolerance Oryza sativa Japonica Group