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Literature summary extracted from

  • Wang, M.; Toda, K.; Maeda, H.A.
    Biochemical properties and subcellular localization of tyrosine aminotransferases in Arabidopsis thaliana (2016), Phytochemistry, 132, 16-25 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.5 expressed in Escherichia coli Rosetta (DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.5 0.204
-
 L-tyrosine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.41
-
 4-hydroxyphenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.455
-
 phenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.74
-
 4-methylthio-2-oxobutanoate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.807
-
 4-methylthio-2-oxobutanoate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 1.28
-
 phenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 1.3
-
 4-methylthio-2-oxobutanoate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 1.7
-
 L-methionine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 2.9
-
 L-tyrosine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 3.06
-
 phenylpyruvate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 3.33
-
 2-oxoglutarate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 3.43
-
 phenylpyruvate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 3.46
-
 4-methylthio-2-oxobutanoate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 6.7
-
 L-phenylalanine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 6.94
-
 L-phenylalanine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 7.62
-
 2-oxoglutarate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 10.1
-
 4-hydroxyphenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 24.2
-
 L-methionine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 42.9
-
 oxaloacetate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.6.1.5 cytosol
-
 Arabidopsis thaliana 5829
-
2.6.1.5 plastid
-
 Arabidopsis thaliana 9536
-

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.5 Arabidopsis thaliana Q67Y55
-
-
2.6.1.5 Arabidopsis thaliana Q9FN30
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.5 HisTrap column chromatography and Sephadex G50 gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.5 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.5 4-hydroxyphenylpyruvate + L-glutamate
-
 Arabidopsis thaliana L-tyrosine + 2-oxoglutarate
-
 r
2.6.1.5 4-hydroxyphenylpyruvate + L-glutamate favoured direction of reaction for isoform TAT2 Arabidopsis thaliana L-tyrosine + 2-oxoglutarate
-
 r
2.6.1.5 4-methylthio-2-oxobutanoate + L-glutamate
-
 Arabidopsis thaliana ?
-
 r
2.6.1.5 L-alanine + 2-oxoglutarate
-
 Arabidopsis thaliana pyruvate + L-glutamate
-
 r
2.6.1.5 L-arginine + 2-oxoglutarate
-
 Arabidopsis thaliana ?
-
 r
2.6.1.5 L-asparagine + 2-oxoglutarate
-
 Arabidopsis thaliana 2-oxosuccinamate + L-glutamate
-
 r
2.6.1.5 L-cysteine + 2-oxoglutarate
-
 Arabidopsis thaliana 3-mercapto-2-oxopropanoate + L-glutamate
-
 r
2.6.1.5 L-glutamine + 2-oxoglutarate
-
 Arabidopsis thaliana 5-amino-2,5-dioxopentanoic acid + L-glutamate
-
 r
2.6.1.5 L-histidine + 2-oxoglutarate
-
 Arabidopsis thaliana 3-(1H-imidazol-4-yl)-2-oxopropanoic acid + L-glutamate
-
 r
2.6.1.5 L-leucine + 2-oxoglutarate
-
 Arabidopsis thaliana 4-methyl-2-oxopentanoic acid + L-glutamate
-
 r
2.6.1.5 L-methionine + 2-oxoglutarate
-
 Arabidopsis thaliana 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
 r
2.6.1.5 L-phenylalanine + 2-oxoglutarate
-
 Arabidopsis thaliana phenylpyruvate + L-glutamate
-
 r
2.6.1.5 L-serine + 2-oxoglutarate
-
 Arabidopsis thaliana 3-hydroxy-2-oxopropanoic acid + L-glutamate
-
 r
2.6.1.5 L-tryptophan + 2-oxoglutarate
-
 Arabidopsis thaliana 3-(1H-indol-3-yl)-2-oxopropanoate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + 2-oxoglutarate
-
 Arabidopsis thaliana 4-hydroxyphenylpyruvate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + 2-oxoglutarate best substrate and favoured direction of reaction for isoform TAT1 Arabidopsis thaliana 4-hydroxyphenylpyruvate + L-glutamate
-
 r
2.6.1.5 L-tyrosine + 4-methylthio-2-oxobutanoate
-
 Arabidopsis thaliana ?
-
 r
2.6.1.5 L-tyrosine + oxaloacetate
-
 Arabidopsis thaliana 4-hydroxyphenylpyruvate + L-aspartate
-
 r
2.6.1.5 L-tyrosine + phenylpyruvate
-
 Arabidopsis thaliana 3-(4-hydroxyphenyl)-2-oxopropanoate + L-phenylalanine
-
 r
2.6.1.5 additional information isoform TAT1 shows no activity with oxaloacetate, L-Ala, L-Ser, L-Cys, L-Asp, L-Asn, L-Gln, and L-Arg Arabidopsis thaliana ?
-
-
2.6.1.5 oxaloacetate + L-glutamate
-
 Arabidopsis thaliana L-aspartate + 2-oxoglutarate
-
 r
2.6.1.5 phenylpyruvate + L-glutamate
-
 Arabidopsis thaliana 2-oxoglutarate + L-phenylalanine
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.5 TAT
-
 Arabidopsis thaliana
2.6.1.5 TAT1 isoform Arabidopsis thaliana
2.6.1.5 TAT2 isoform Arabidopsis thaliana
2.6.1.5 Tyr aminotransferase
-
 Arabidopsis thaliana
2.6.1.5 tyrosine aminotransferase
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.5 0.13
-
 phenylpyruvate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.37
-
 4-methylthio-2-oxobutanoate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.43
-
 4-hydroxyphenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.47
-
 phenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.51
-
 L-phenylalanine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.53
-
 L-methionine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.53
-
 2-oxoglutarate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.56
-
 oxaloacetate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.7
-
 4-methylthio-2-oxobutanoate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.75
-
 L-tyrosine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 18.5
-
 4-methylthio-2-oxobutanoate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 20.1
-
 4-methylthio-2-oxobutanoate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 23.9
-
 4-hydroxyphenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 24.5
-
 phenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 26.6
-
 L-methionine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 30.2
-
 phenylpyruvate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 31.6
-
 L-phenylalanine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 48
-
 2-oxoglutarate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 57.3
-
 L-tyrosine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.5 8.5 9
-
 Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.5 0.02
-
 oxaloacetate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.04
-
 phenylpyruvate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.07
-
 2-oxoglutarate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.08
-
 L-phenylalanine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.23
-
 L-tyrosine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.32
-
 L-methionine isoform TAT2, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.49
-
 phenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 0.51
-
 4-methylthio-2-oxobutanoate isoform TAT2, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 1.1
-
 L-methionine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 1.15
-
 4-methylthio-2-oxobutanoate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 1.38
-
 4-hydroxyphenylpyruvate isoform TAT2, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 2.5
-
 4-hydroxyphenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 4.5
-
 L-phenylalanine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 5.5
-
 4-methylthio-2-oxobutanoate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 9.9
-
 phenylpyruvate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 15.1
-
 2-oxoglutarate isoform TAT1, with L-tyrosine as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 15.5
-
 4-methylthio-2-oxobutanoate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 54.2
-
 phenylpyruvate isoform TAT1, with L-glutamate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana
2.6.1.5 281.9
-
 L-tyrosine isoform TAT1, with 2-oxoglutarate as cosubstrate, at pH 8.5 and 30°C Arabidopsis thaliana