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Literature summary extracted from

  • Koivulehto, M.; Battchikova, N.; Korpela, S.; Khalikova, E.; Zavialov, A.; Korpela, T.
    Comparison of kinetic and enzymatic properties of intracellular phosphoserine aminotransferases from alkaliphilic and neutralophilic bacteria (2020), Open Chem., 18, 149-164 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.52 gene serC, recombinant overexpression in Escherichia coli strain AB2829 harbouring pKD501 plasmid Escherichia coli
2.6.1.52 gene serC, recombinant overexpression in Escherichia coli strain BL21(DE3) Alkalihalobacillus alcalophilus
2.6.1.52 gene serC, recombinant overexpression of enzyme mutant K2G in Escherichia coli strain XL-1 Blue Niallia circulans

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.52 K2G site-directed mutagenesis Niallia circulans
2.6.1.52 additional information mutant C2-A' Tetradesmus obliquus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.52 additional information
-
 additional information enzyme kinetics, overview Niallia circulans
2.6.1.52 additional information
-
 additional information enzyme kinetics, overview Alkalihalobacillus alcalophilus
2.6.1.52 additional information
-
 additional information enzyme kinetics, overview Escherichia coli
2.6.1.52 0.0017
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme mutant K2G Niallia circulans
2.6.1.52 0.004
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme Escherichia coli
2.6.1.52 0.005
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme Bos taurus
2.6.1.52 0.0173
-
 3-phosphooxypyruvate pH 9.5, 25°C, recombinant enzyme mutant K2G Niallia circulans
2.6.1.52 0.06
-
 3-phosphooxypyruvate pH 7.5-9.0, 25°C, recombinant enzyme Glycine max
2.6.1.52 0.25
-
 3-phosphooxypyruvate pH 8.2, 25°C, recombinant enzyme Ovis aries
2.6.1.52 0.5
-
 L-glutamate pH 7.5-9.0, 25°C, recombinant enzyme Glycine max
2.6.1.52 0.8
-
 L-glutamate pH 9.5, 25°C, recombinant enzyme mutant K2G Niallia circulans
2.6.1.52 1.2
-
 L-glutamate pH 8.2, 25°C, recombinant enzyme Escherichia coli
2.6.1.52 1.2
-
 L-glutamate pH 8.2, 25°C, recombinant enzyme Bos taurus
2.6.1.52 1.4
-
 L-glutamate pH 8.2, 25°C, recombinant enzyme mutant K2G Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.52 66000
-
 recombinant enzyme K2G, gel filtration Niallia circulans
2.6.1.52 68000
-
 gel filtration Escherichia coli
2.6.1.52 77000
-
 gel filtration Bos taurus
2.6.1.52 79800 81200
-
 Escherichia coli
2.6.1.52 80000
-
 gel filtration Tetradesmus obliquus
2.6.1.52 85000
-
 gel filtration Glycine max
2.6.1.52 90700
-
 sedimentation equilibrium Bos taurus
2.6.1.52 96000
-
 sedimentation equilibrium Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Niallia circulans
-
 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Alkalihalobacillus alcalophilus
-
 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Escherichia coli
-
 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Bos taurus
-
 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Ovis aries
-
 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Tetradesmus obliquus
-
 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate Glycine max
-
 3-phosphooxypyruvate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.52 Alkalihalobacillus alcalophilus Q9RME2 i.e. Bacillus alcalophilus
-
2.6.1.52 Bos taurus A0A3S5ZPF7
-
-
2.6.1.52 Escherichia coli E2QJB8
-
-
2.6.1.52 Escherichia coli P23721
-
-
2.6.1.52 Glycine max I1JCA9
-
-
2.6.1.52 Niallia circulans Q59196 var. alcalophilus
-
2.6.1.52 Ovis aries W5PS11
-
-
2.6.1.52 Tetradesmus obliquus Q9S8N7 i.e. Acutodesmus obliquus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.52 recombinant enzyme 15.6fold from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography, ultrafiltration, and gel filtration Alkalihalobacillus alcalophilus
2.6.1.52 recombinant enzyme mutant K2G 5.5fold from Escherichia coli strain XL-1 Blue by anion exchange chromatography, ultrafiltration, and gel filtration Niallia circulans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.52 brain
-
 Ovis aries
-
2.6.1.52 liver
-
 Bos taurus
-
2.6.1.52 additional information the alkaliphilic microbe grows optimally at pH 9.5-10.5 Niallia circulans
-
2.6.1.52 root nodule
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.52 1.3
-
 purified enzyme, pH 8.1-8.2, 25°C Ovis aries
2.6.1.52 3.8
-
 pH not specified in the publication, 25°C, purified recombinant enzyme, substrate 3-phosphooxypyruvate Escherichia coli
2.6.1.52 7
-
 purified enzyme, pH and temperature not specified in the publication Glycine max
2.6.1.52 42
-
 purified recombinant enzyme, pH 6.1, 25°C Alkalihalobacillus alcalophilus
2.6.1.52 42.3
-
 purified recombinant enzyme, pH 8.2, 25°C Escherichia coli
2.6.1.52 44.4
-
 purified recombinant enzyme mutant K2G, pH 6.1, 25°C Niallia circulans
2.6.1.52 1416
-
 purified enzyme, pH 6.8-7.2, 38°C Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Niallia circulans O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Alkalihalobacillus alcalophilus O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Escherichia coli O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Bos taurus O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Ovis aries O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Tetradesmus obliquus O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 3-phosphooxypyruvate + L-glutamate
-
 Glycine max O-phospho-L-serine + 2-oxoglutarate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Niallia circulans 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Alkalihalobacillus alcalophilus 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Escherichia coli 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Bos taurus 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Ovis aries 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Tetradesmus obliquus 3-phosphooxypyruvate + L-glutamate
-
 r
2.6.1.52 O-phospho-L-serine + 2-oxoglutarate
-
 Glycine max 3-phosphooxypyruvate + L-glutamate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.52 ? x * 39500-40500, recombinant enzyme, SDS-PAGE, x * 40200, about, sequence calculation Alkalihalobacillus alcalophilus
2.6.1.52 homodimer 2 * 40000, SDS-PAGE Tetradesmus obliquus
2.6.1.52 homodimer 2 * 43000, SDS-PAGE Bos taurus
2.6.1.52 homodimer 2 * 38000-39800, recombinant enzyme mutant K2G, SDS-PAGE, 2 * 39793, sequence calculation Niallia circulans
2.6.1.52 homodimer 2 * 39000-40000, SDS-PAGE, 2 * 39834, sequence calculation Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.52 phosphoserine aminotransferases
-
 Niallia circulans
2.6.1.52 phosphoserine aminotransferases
-
 Alkalihalobacillus alcalophilus
2.6.1.52 phosphoserine aminotransferases
-
 Escherichia coli
2.6.1.52 phosphoserine aminotransferases
-
 Bos taurus
2.6.1.52 phosphoserine aminotransferases
-
 Ovis aries
2.6.1.52 phosphoserine aminotransferases
-
 Tetradesmus obliquus
2.6.1.52 phosphoserine aminotransferases
-
 Glycine max
2.6.1.52 PSAT
-
 Niallia circulans
2.6.1.52 PSAT
-
 Alkalihalobacillus alcalophilus
2.6.1.52 PSAT
-
 Escherichia coli
2.6.1.52 PSAT
-
 Bos taurus
2.6.1.52 PSAT
-
 Ovis aries
2.6.1.52 PSAT
-
 Tetradesmus obliquus
2.6.1.52 PSAT
-
 Glycine max
2.6.1.52 PSAT-BALC
-
 Alkalihalobacillus alcalophilus
2.6.1.52 PSAT-BCIRA
-
 Niallia circulans
2.6.1.52 PSAT-ECOLI
-
 Escherichia coli
2.6.1.52 PSAT1
-
 Bos taurus
2.6.1.52 serC
-
 Niallia circulans
2.6.1.52 serC
-
 Alkalihalobacillus alcalophilus
2.6.1.52 serC
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.52 25
-
 assay at Niallia circulans
2.6.1.52 25
-
 assay at Alkalihalobacillus alcalophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.52 6.8 7.2
-
 Bos taurus
2.6.1.52 6.8 8.2
-
 Tetradesmus obliquus
2.6.1.52 7.5 9 broad optimum Glycine max
2.6.1.52 7.5 8.5
-
 Escherichia coli
2.6.1.52 8.1 8.2
-
 Ovis aries
2.6.1.52 9.4
-
-
 Alkalihalobacillus alcalophilus
2.6.1.52 9.5
-
-
 Niallia circulans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.52 6.5 12 activity range, profile overview Niallia circulans
2.6.1.52 6.5 12 activity range, profile overview Alkalihalobacillus alcalophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Niallia circulans
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Alkalihalobacillus alcalophilus
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Escherichia coli
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Bos taurus
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Ovis aries
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Tetradesmus obliquus
2.6.1.52 pyridoxal 5'-phosphate PLP, dependent on Glycine max

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.52 Niallia circulans isoelectric focusing
-
 4.6
2.6.1.52 Alkalihalobacillus alcalophilus isoelectric focusing
-
 4.6
2.6.1.52 Escherichia coli isoelectric focusing
-
 4.6

General Information

EC Number General Information Comment Organism
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Niallia circulans
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Alkalihalobacillus alcalophilus
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Escherichia coli
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Bos taurus
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Ovis aries
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Tetradesmus obliquus
2.6.1.52 metabolism enzyme PSAT catalyses the second step of phosphorylated pathway of serine biosynthesis Glycine max