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Literature summary extracted from

  • Yu, H.; Takeuchi, M.; LeBarron, J.; Kantharia, J.; London, E.; Bakker, H.; Haltiwanger, R.; Li, H.; Takeuchi, H.
    Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism (2015), Nat. Chem. Biol., 11, 847-854 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.62 expressed in HEK-293T cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.62 in complex with acceptor Xyl-Glc-[EGF], hanging drop vapor diffusion method, using 0.2 M Li2SO4, 0.1 M Bis-Tris, pH 6.5, and 21% (w/v) PEG3350 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.62 membrane
-
 Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.62 Mn2+ required for activity Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.62 UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine Mus musculus
-
 UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.62 Mus musculus Q3U4G3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.62 Ni-NTA column chromatography and Superdex S200 gel filtration Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.62 UDP-alpha-D-xylose + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
-
 Mus musculus UDP + [protein with EGF-like domain]-3-O-[alpha-D-xylosyl-(1->3)-alpha-D-xylosyl-(1->3)-beta-D-glucosyl]-L-serine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.62 XXYLT1
-
 Mus musculus
2.4.2.62 xyloside alpha1-3 xylosyltransferase
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.4.2.62 metabolism the enzyme transfers xylose from the donor UDP-alpha-D-xylose to Xyl-Glc disaccharide-modified EGF repeats from Notch1, Notch2 or human factor IX Mus musculus