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Literature summary extracted from

  • Chen, F.F.; Zhang, Y.H.; Zhang, Z.J.; Liu, L.; Wu, J.P.; Xu, J.H.; Zheng, G.W.
    An ammonium-formate-driven trienzymatic cascade for omega-transaminase-catalyzed (R)-selective amination (2019), J. Org. Chem., 84, 14987-14993 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.B21 synthesis development of a one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products Aspergillus terreus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.B21 recombinant expression in Escherichia coli strain BL21(DE3) Aspergillus terreus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.B21 Aspergillus terreus Q0C8G1
-
-
2.6.1.B21 Aspergillus terreus FGSC A1156 Q0C8G1
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-
2.6.1.B21 Aspergillus terreus NIH 2624 Q0C8G1
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.B21 additional information (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview Aspergillus terreus ?
-
-
2.6.1.B21 additional information (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview Aspergillus terreus NIH 2624 ?
-
-
2.6.1.B21 additional information (R)-amination mediated by (R)-specific omega-transaminases generally requires costly D-alanine in excess to obtain the desired chiral amines in high yield. A one-pot, trienzymatic cascade comprising an (R)-specific omega-transaminase, an amine dehydrogenase, and a formate dehydrogenase is developed for the economical and ecofriendly synthesis of (R)-chiral amines. Using inexpensive ammonium formate as the sole sacrificial agent, the established cascade system enables efficient omega-transaminase-mediated (R)-amination of various ketones, with high conversions and excellent enantiomeric excess (over 99%), water and CO2 are the only waste products. The wild-type enzyme shows broad substrate specificity and enantioselectivity. Cascade reaction, overview Aspergillus terreus FGSC A1156 ?
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-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.B21 (R)-amine:pyruvate transaminase
-
 Aspergillus terreus
2.6.1.B21 (R)-omega-TA
-
 Aspergillus terreus
2.6.1.B21 (R)-selective aminotransferase
-
 Aspergillus terreus
2.6.1.B21 (R)-selective omega-TA
-
 Aspergillus terreus
2.6.1.B21 At-omega-TA
-
 Aspergillus terreus
2.6.1.B21 ATEG_10023 locus name Aspergillus terreus
2.6.1.B21 omega-transaminase
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.B21 30
-
 assay at Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.B21 8.8
-
 assay at Aspergillus terreus

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.B21 pyridoxal 5'-phosphate
-
 Aspergillus terreus