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Literature summary extracted from

  • Rzad, K.; Milewski, S.; Gabriel, I.
    Versatility of putative aromatic aminotransferases from Candida albicans (2018), Fungal Genet. Biol., 110, 26-37 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.57 gene aro8, recombinant overexpression in Escherichia coli strain Rosetta (DE3) pLysS, subcloning in Escherichia coli strain TOP10F' Candida albicans
2.6.1.57 gene aro9, recombinant overexpression in Escherichia coli strain Rosetta (DE3) pLysS, subcloning in Escherichia coli strain TOP10F' Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.57 additional information construction of a DELTAaro9 knockout strain, also of a double mutant with aro8, overview Candida albicans
2.6.1.57 additional information construction of a DELTAyer152C knockout strain, also of a double mutant with aro8, overview Candida albicans
2.6.1.57 additional information construction of several DELTAaro8 knockout strains, also double mutants with aro9 and yer152C, genotypes, overview Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.57 (aminooxy)acetate AOA, is a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, such as transaminations. AOA strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner; AOA, is a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, such as transaminations. AOA strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner; AOA, is a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, such as transaminations. AOA strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.57 0.016
-
 alpha-aminoadipate pH and temperature not specified in the publication, with 2-oxoglutarate Candida albicans
2.6.1.57 0.017
-
 phenylpyruvate pH and temperature not specified in the publication, with L-glutamate Candida albicans
2.6.1.57 0.026
-
 4-hydroxyphenylpyruvate pH and temperature not specified in the publication, with L-glutamate Candida albicans
2.6.1.57 0.053
-
 L-phenylalanine pH and temperature not specified in the publication, with 2-oxoglutarate Candida albicans
2.6.1.57 0.09
-
 L-tyrosine pH and temperature not specified in the publication, with 2-oxoglutarate Candida albicans
2.6.1.57 0.1
-
 2-oxoadipate pH and temperature not specified in the publication, with L-glutamate Candida albicans
2.6.1.57 0.13
-
 2-oxoadipate pH and temperature not specified in the publication, with L-glutamate Candida albicans
2.6.1.57 0.24
-
 L-phenylalanine pH and temperature not specified in the publication Candida albicans
2.6.1.57 0.27
-
 2-oxoglutarate pH and temperature not specified in the publication Candida albicans
2.6.1.57 0.41
-
 2-oxoglutarate pH and temperature not specified in the publication Candida albicans
2.6.1.57 0.46
-
 L-tyrosine pH and temperature not specified in the publication Candida albicans
2.6.1.57 1.07
-
 L-tryptophan pH and temperature not specified in the publication Candida albicans
2.6.1.57 1.68
-
 L-tryptophan pH and temperature not specified in the publication, with 2-oxoglutarate Candida albicans
2.6.1.57 1.8
-
 L-tryptophan pH and temperature not specified in the publication Candida albicans
2.6.1.57 2.8
-
 L-tyrosine pH and temperature not specified in the publication Candida albicans
2.6.1.57 4.28
-
 alpha-aminoadipate pH and temperature not specified in the publication Candida albicans
2.6.1.57 4.76
-
 L-phenylalanine pH and temperature not specified in the publication Candida albicans
2.6.1.57 11.5
-
 L-histidine pH and temperature not specified in the publication, with 2-oxoglutarate Candida albicans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.57 2-oxoglutarate + L-phenylalanine Candida albicans
-
 L-glutamate + phenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-phenylalanine Candida albicans ATCC MYA-2876
-
 L-glutamate + phenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-tryptophan Candida albicans
-
 L-glutamate + 3-indole-2-oxopropanoate
-
 r
2.6.1.57 2-oxoglutarate + L-tryptophan Candida albicans ATCC MYA-2876
-
 L-glutamate + 3-indole-2-oxopropanoate
-
 r
2.6.1.57 2-oxoglutarate + L-tyrosine Candida albicans
-
 L-glutamate + 4-hydroxyphenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-tyrosine Candida albicans ATCC MYA-2876
-
 L-glutamate + 4-hydroxyphenylpyruvate
-
 r
2.6.1.57 alpha-aminoadipate + 2-oxoglutarate Candida albicans
-
 2-oxoadipate + L-glutamate
-
 r
2.6.1.57 alpha-aminoadipate + 2-oxoglutarate Candida albicans ATCC MYA-2876
-
 2-oxoadipate + L-glutamate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.57 Candida albicans A0A1D8PG20
-
-
2.6.1.57 Candida albicans A0A1D8PMC5
-
-
2.6.1.57 Candida albicans A0A1D8PPA8
-
-
2.6.1.57 Candida albicans ATCC MYA-2876 A0A1D8PG20
-
-
2.6.1.57 Candida albicans ATCC MYA-2876 A0A1D8PMC5
-
-
2.6.1.57 Candida albicans ATCC MYA-2876 A0A1D8PPA8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.57 2-oxoadipate + L-glutamate
-
 Candida albicans alpha-aminoadipate + 2-oxoglutarate
-
 r
2.6.1.57 2-oxoglutarate + L-histidine
-
 Candida albicans L-glutamate + 3-(1H-imidazol-4-yl)-2-oxopropanoate
-
 r
2.6.1.57 2-oxoglutarate + L-phenylalanine
-
 Candida albicans L-glutamate + phenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-phenylalanine
-
 Candida albicans ATCC MYA-2876 L-glutamate + phenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-tryptophan
-
 Candida albicans L-glutamate + 3-indole-2-oxopropanoate
-
 r
2.6.1.57 2-oxoglutarate + L-tryptophan
-
 Candida albicans ATCC MYA-2876 L-glutamate + 3-indole-2-oxopropanoate
-
 r
2.6.1.57 2-oxoglutarate + L-tyrosine
-
 Candida albicans L-glutamate + 4-hydroxyphenylpyruvate
-
 r
2.6.1.57 2-oxoglutarate + L-tyrosine
-
 Candida albicans ATCC MYA-2876 L-glutamate + 4-hydroxyphenylpyruvate
-
 r
2.6.1.57 alpha-aminoadipate + 2-oxoglutarate
-
 Candida albicans 2-oxoadipate + L-glutamate
-
 r
2.6.1.57 alpha-aminoadipate + 2-oxoglutarate
-
 Candida albicans ATCC MYA-2876 2-oxoadipate + L-glutamate
-
 r
2.6.1.57 L-glutamate + 4-hydroxyphenylpyruvate
-
 Candida albicans 2-oxoglutarate + L-tyrosine
-
 r
2.6.1.57 L-glutamate + 4-hydroxyphenylpyruvate
-
 Candida albicans ATCC MYA-2876 2-oxoglutarate + L-tyrosine
-
 r
2.6.1.57 L-glutamate + phenylpyruvate
-
 Candida albicans 2-oxoglutarate + L-phenylalanine
-
 r
2.6.1.57 L-glutamate + phenylpyruvate
-
 Candida albicans ATCC MYA-2876 2-oxoglutarate + L-phenylalanine
-
 r
2.6.1.57 additional information no activity with L-histidine Candida albicans ?
-
-
2.6.1.57 additional information no activity with L-histidine Candida albicans ATCC MYA-2876 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
2.6.1.57 Aro8
-
 Candida albicans
2.6.1.57 ARO9
-
 Candida albicans
2.6.1.57 aromatic-amino-acid:2-oxoglutarate transaminase UniProt Candida albicans
2.6.1.57 bifunctional 2-aminoadipate transaminase/aromatic-amino-acid:2-oxoglutarate transaminase UniProt Candida albicans
2.6.1.57 CaAro8p
-
 Candida albicans
2.6.1.57 CaAro9p
-
 Candida albicans
2.6.1.57 CaYer152Cp
-
 Candida albicans
2.6.1.57 Yer152C
-
 Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.57 pyridoxal 5'-phosphate PLP Candida albicans

General Information

EC Number General Information Comment Organism
2.6.1.57 malfunction inhibition of transaminase enzymes by (aminooxy)acetate (AOA), a broad-spectrum inhibitor of pyridoxal phosphate-dependent enzymatic reactions, strongly inhibits the growth of Candida albicans in minimal medium with selected amino acids as the sole nitrogen source in an amino acid concentration-dependent manner Candida albicans
2.6.1.57 metabolism enzyme CaAro8p is involved in the catabolism of histidine, lysine, and aromatic amino acids as well as in L-Lys, L-Phe, and L-Tyr biosynthesis Candida albicans
2.6.1.57 metabolism enzyme Cayer152Cp is active with aromatic amino acids but does not play an important role in vivo in aromatic acid catabolism and biosynthesis Candida albicans
2.6.1.57 metabolism the enzyme CaAro9p participates in the catabolism of aromatic amino acids and lysine at high concentrations of these compounds, with no biosynthetic role Candida albicans