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Literature summary extracted from

  • Liu, X.; Lin, C.; Ma, X.; Tan, Y.; Wang, J.; Zeng, M.
    Functional characterization of a flavonoid glycosyltransferase in sweet orange (Citrus sinensis) (2018), Front. Plant Sci., 9, 166 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.185 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression in Nicotiana tabacum using the Agrobacterium tumefaciens strain EHA105 transfection method Citrus sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.185 0.01516
-
 hesperetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.02041
-
 naringenin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.02809
-
 kaempferol pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.03678
-
 quercetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.04327
-
 diosmetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.185 additional information Citrus sinensis the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo ?
-
-
2.4.1.185 UDP-glucose + a flavanone Citrus sinensis
-
 UDP + a flavanone 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + a flavone Citrus sinensis
-
 UDP + a flavone 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + a flavonol Citrus sinensis
-
 UDP + a flavonol 7-O-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.185 Citrus sinensis
-
 cv. Valencia
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.185 fruit relative expression of CsUGT76F1 in sweet orange peels and pulp during fruit development, overview Citrus sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.185 additional information the enzyme has flavonoid 7-O-glucosyltransferase and 7-O-rhamnosyltransferase activities in vivo Citrus sinensis ?
-
-
2.4.1.185 additional information in vitro, enzyme CsUGT76F1 can function as flavonoid 3-O-, 7-O-, and 3,7-O-glucosyltransferase, and as 7-O-rhamnosyltransferase with a broad range of flavonoid substrates that can be glucosylated at their 3- and/or 7-hydrogen sites by the recombinant enzyme, including flavanones, flavonols, and flavons, e.g. hesperetin, naringenin, diosmetin, quercetin, and kaempferol as acceptor substrates, and UDP-glucose and UDP-rhamnose as the sugar donors. Mass spectrometric product analysis (UPLC-Q-TOF-MS). The recombinant UGT can only accept UDP-rhamnose to rhamnosylate the 7-hydrogen position of quercetin and kaempferol, but not to catalyze the rhamnosylation of hesperetin, naringenin, and diosmetin. No flavonoid O-rutinoside (rhamnosyl-alpha-1,6-glucose) or O-neohesperidose (rhamnosyl-alpha-1,2-glucose) products are detected Citrus sinensis ?
-
-
2.4.1.185 UDP-glucose + a flavanone
-
 Citrus sinensis UDP + a flavanone 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + a flavone
-
 Citrus sinensis UDP + a flavone 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + a flavonol
-
 Citrus sinensis UDP + a flavonol 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + diosmetin
-
 Citrus sinensis UDP + diosmetin 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + eriodictyol
-
 Citrus sinensis UDP + eriodictyol 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + hesperetin
-
 Citrus sinensis UDP + hesperetin 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + isosakuranetin
-
 Citrus sinensis UDP + isosakuranetin 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + kaempferol the enzyme can form kaempferol 3,7-O-diglucoside Citrus sinensis UDP + kaempferol 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + kaempferol the enzyme can form kaempferol 3,7-O-diglucoside, reaction of EC 2.4.1.91 Citrus sinensis UDP + kaempferol 3-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + naringenin
-
 Citrus sinensis UDP + naringenin 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + quercetin
-
 Citrus sinensis UDP + quercetin 7-O-beta-D-glucoside
-
 ?
2.4.1.185 UDP-glucose + quercetin reaction of EC 2.4.1.91 Citrus sinensis UDP + quercetin 3-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.185 CsUGT76F1
-
 Citrus sinensis
2.4.1.185 flavonoid glycosyltransferase
-
 Citrus sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.185 35 40 dependent on the substrate Citrus sinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.185 10 70 activity range Citrus sinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.185 0.46
-
 kaempferol pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.58
-
 quercetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.71
-
 naringenin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 0.77
-
 hesperetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 1.6
-
 diosmetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.185 7.5 8 dependent on the substrate Citrus sinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.185 4 11 activity range Citrus sinensis

General Information

EC Number General Information Comment Organism
2.4.1.185 malfunction overexpression of the gene significantly increases the accumulations of quercetin 7-O-rhamnoside, quercetin 7-O-glucoside, and kaempferol 7-O-glucoside Citrus sinensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.185 15.77
-
 quercetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 16.38
-
 kaempferol pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 34.79
-
 naringenin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 36.98
-
 diosmetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis
2.4.1.185 50.79
-
 hesperetin pH 7.5, 30°C, with UDP-glucose, recombinant enzyme Citrus sinensis