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Literature summary extracted from

  • Chitnumsub, P.; Jaruwat, A.; Talawanich, Y.; Noytanom, K.; Liwnaree, B.; Poen, S.; Yuthavong, Y.
    The structure of Plasmodium falciparum hydroxymethyldihydropterin pyrophosphokinase-dihydropteroate synthase reveals the basis of sulfa resistance (2020), FEBS J., 287, 3273-3297 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.15 expressed in Escherichia coli Rosetta(DE3)pLysS cells Plasmodium falciparum

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.15 HPPK-DHPS from wild type and sulfa-resistant parasites, in complex with pteroate, sulfathiazole-dihydropteroate, and sulfadoxine-dihydropteroate, microbatch method, using 0.1 mM bicine pH 9.0, 0.5-0.6 M calcium acetate-Ca(OAc)2, 20% (w/v) PEG 4000 at 25°C Plasmodium falciparum
2.5.1.15 structures of the enzyme from wild-type and sulfa-resistant mutants, both as apoenzyme and as complexes with pteroate and sulfa derivatives. Pteroate stays in active sites without steric constraint. In contrast, parts of the sulfa compounds situated outside of the substrate envelope are in the vicinity of the resistance mutations. Steric conflict between compound and mutant residue along with increased flexibility of loop D2 in the mutants can account for the reduced compound binding affinity to the mutants Plasmodium falciparum
2.7.6.3 structures of the enzyme from wild-type and sulfa-resistant mutants, both as apoenzyme and as complexes with pteroate and sulfa derivatives. Pteroate stays in active sites without steric constraint. In contrast, parts of the sulfa compounds situated outside of the substrate envelope are in the vicinity of the resistance mutations. Steric conflict between compound and mutant residue along with increased flexibility of loop D2 in the mutants can account for the reduced compound binding affinity to the mutants Plasmodium falciparum

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.15 A437G mutant enzyme shows increased affinity for 4-aminobenzoate along with reduced binding and efficacy of sulfa drugs. (kcat/Km) for 4-aminobenzoate is increased by 6fold. Mutation enhances the dynamic flexibility of loop D2 Plasmodium falciparum
2.5.1.15 A437G/K540E the mutations slightly decrease the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 A581G the mutation strongly decreases the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 A613S the mutation strongly decreases the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 A613T the mutation slightly decreases the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 K540E the mutation strongly decreases the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 S436A/A437G the mutations increase the catalytic efficiency (kcat/Km) of the enzyme by 1.6fold with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 S436F/A437G/A613S the mutations increase the catalytic efficiency (kcat/Km) of the enzyme by 2fold with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 S436F/A437G/A613T the triple mutant exhibits lower Km value for 4-aminobenzoate than the wild type enzyme. The mutations slightly increase the catalytic efficiency (kcat/Km) of the enzyme Plasmodium falciparum
2.5.1.15 S436F/A613S the mutations strongly decrease the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.5.1.15 S436F/A613T the mutations strongly decrease the catalytic efficiency (kcat/Km) of the enzyme with 4-aminobenzoate as substrate Plasmodium falciparum
2.7.6.3 A437G 6fold increase in kcat/Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 A437G/K540E decrease in Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 A581G decrease in Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 A613S decrease in Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 A613T decrease in Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 K540E decrease in Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 S436A/A437G 1.6fold increase in kcat/Km value for 4-aminobenzoate Plasmodium falciparum
2.7.6.3 S436F/A437G/A613S 2fold increase in kcat/Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 S436F/A437G/A613T kinetic values similar to wild-type Plasmodium falciparum
2.7.6.3 S436F/A613S decrease in Km value of 4-aminobenzoate Plasmodium falciparum
2.7.6.3 S436F/A613T decrease in Km value of 4-aminobenzoate Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.15 dapsone inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 Pteroate inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 sulfadimethoxine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 sulfadoxine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 sulfamethazine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 sulfamethoxazole inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 sulfamonomethoxine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.5.1.15 sulfathiazole inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 dapsone inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 Pteroate inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 sulfadimethoxine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 sulfadoxine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 sulfamethazine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 sulfamethoxazole inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 sulfamonomethoxine inhibition of dihydropteroate synthase activity Plasmodium falciparum
2.7.6.3 sulfathiazole inhibition of dihydropteroate synthase activity Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.15 0.00033
-
 4-Aminobenzoate mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00034
-
 4-Aminobenzoate mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00046
-
 4-Aminobenzoate mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00062
-
 4-Aminobenzoate mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00101
-
 4-Aminobenzoate mutant enzyme A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00132
-
 4-Aminobenzoate mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00149
-
 4-Aminobenzoate wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00151
-
 4-Aminobenzoate mutant enzyme K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00217
-
 4-Aminobenzoate mutant enzyme S436F/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00223
-
 4-Aminobenzoate mutant enzyme A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00245
-
 4-Aminobenzoate mutant enzyme S436F/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.00443
-
 4-Aminobenzoate mutant enzyme A581G, at pH 9.0 and 37°C Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.15 Mg2+ 10 mM used in assay conditions Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.15 86000
-
-
 Plasmodium falciparum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.15 (7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate Plasmodium falciparum
-
 7,8-dihydropteroate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.15 Plasmodium falciparum Q25704 bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) and dihydropteroate synthase, cf. EC 2.7.6.3
-
2.7.6.3 Plasmodium falciparum Q25704 bifunctional 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) and dihydropteroate synthase, cf. EC 2.5.1.5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.15 Ni-Sepharose column chromatography Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.15 (7,8-dihydropterin-6-yl)methyl diphosphate + 4-aminobenzoate
-
 Plasmodium falciparum 7,8-dihydropteroate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.15 ? x * 86000, SDS-PAGE Plasmodium falciparum

Synonyms

EC Number Synonyms Comment Organism
2.5.1.15 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase-dihydropteroate synthase bifunctional enzyme Plasmodium falciparum
2.5.1.15 7,8-dihydropteroate synthase
-
 Plasmodium falciparum
2.5.1.15 HPPK-DHPS bifunctional enzyme with hydroxymethyldihydropterin diphosphokinase and dihydropteroate synthase activities Plasmodium falciparum
2.5.1.15 PPPK-DHPS
-
 Plasmodium falciparum
2.7.6.3 PPPK-DHPS cf. EC 2.5.1.5 Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.15 0.0057
-
 4-Aminobenzoate mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0075
-
 4-Aminobenzoate mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0077
-
 4-Aminobenzoate mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.01
-
 4-Aminobenzoate mutant enzyme A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.01
-
 4-Aminobenzoate mutant enzyme S436F/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.011
-
 4-Aminobenzoate mutant enzyme K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.012
-
 4-Aminobenzoate mutant enzyme A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0137
-
 4-Aminobenzoate mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.014
-
 4-Aminobenzoate mutant enzyme S436F/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.015
-
 4-Aminobenzoate wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.02
-
 4-Aminobenzoate mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.029
-
 4-Aminobenzoate mutant enzyme A581G, at pH 9.0 and 37°C Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.15 0.0005
-
 sulfathiazole wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0006
-
 Pteroate mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0006
-
 sulfamonomethoxine wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0008
-
 dapsone wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0008
-
 sulfamethazine wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0008
-
 sulfadimethoxine wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0009
-
 Pteroate mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.001
-
 Pteroate mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0011
-
 dapsone mutant enzyme K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0014
-
 dapsone mutant enzyme A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0014
-
 Pteroate wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0016
-
 sulfathiazole mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0016
-
 sulfamethazine mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0017
-
 Pteroate mutant enzyme S436F/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0018
-
 sulfamonomethoxine mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0019
-
 dapsone mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0024
-
 sulfadimethoxine mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.003
-
 Pteroate mutant enzyme A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.003
-
 Pteroate mutant enzyme K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0033
-
 Pteroate mutant enzyme A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0037
-
 Pteroate mutant enzyme S436F/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0038
-
 Pteroate mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0039
-
 dapsone mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0052
-
 sulfadoxine mutant enzyme A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0069
-
 Pteroate mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.007
-
 sulfadoxine mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.007
-
 dapsone mutant enzyme A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0071
-
 sulfamethazine mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0071
-
 sulfadoxine wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0072
-
 sulfathiazole mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0091
-
 sulfamonomethoxine mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.0092
-
 sulfadoxine mutant enzyme A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.014
-
 sulfadimethoxine mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.014
-
 sulfamonomethoxine mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.016
-
 sulfadimethoxine mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.018
-
 sulfathiazole mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.02
-
 sulfadoxine mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.021
-
 sulfamethazine mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.023
-
 sulfadoxine mutant enzyme K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.025
-
 sulfathiazole mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.027
-
 sulfathiazole mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.034
-
 sulfamethoxazole wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.035
-
 sulfamonomethoxine mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.039
-
 Pteroate mutant enzyme A581G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.04
-
 dapsone mutant enzyme A581G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.04
-
 sulfadimethoxine mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.058
-
 dapsone mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.058
-
 sulfamethazine mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.07
-
 sulfadoxine mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.084
-
 sulfadoxine mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.086
-
 sulfadoxine mutant enzyme S436F/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.116
-
 sulfadimethoxine mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.124
-
 sulfadoxine mutant enzyme S436F/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.153
-
 sulfamethoxazole mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.156
-
 dapsone mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.177
-
 dapsone mutant enzyme S436F/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.192
-
 dapsone mutant enzyme S436F/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.196
-
 sulfadoxine mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.198
-
 sulfadoxine mutant enzyme A581G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.462
-
 sulfamethoxazole mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.48
-
 sulfamethoxazole mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 0.731
-
 sulfamethoxazole mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.7.6.3 0.0006
-
 Pteroate mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0006
-
 sulfamonomethoxine wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0007
-
 dapsone mutant A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0008
-
 dapsone wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0008
-
 sulfamethazine wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0008
-
 sulfadimethoxine wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0009
-
 Pteroate mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.001
-
 Pteroate mutant S436A/A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0011
-
 dapsone mutant K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0014
-
 dapsone mutant A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0014
-
 Pteroate wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0016
-
 sulfathiazole mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0016
-
 sulfamethazine mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0017
-
 Pteroate mutant S436F/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0018
-
 sulfamonomethoxine mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0019
-
 dapsone mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0024
-
 sulfadimethoxine mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.003
-
 Pteroate mutant K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0033
-
 Pteroate mutant A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0037
-
 Pteroate mutant S436F/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0038
-
 Pteroate mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0039
-
 dapsone mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.005
-
 sulfathiazole wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0052
-
 sulfadoxine mutant A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0069
-
 Pteroate mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.007
-
 sulfadoxine mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0071
-
 sulfamethazine mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0071
-
 sulfadoxine wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0072
-
 sulfathiazole mutant S436A/A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0091
-
 sulfamonomethoxine mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.0092
-
 sulfadoxine mutant A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.014
-
 sulfadimethoxine mutant S436A/A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.014
-
 sulfamonomethoxine mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.016
-
 sulfadimethoxine mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.018
-
 sulfathiazole mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.02
-
 sulfadoxine mutant S436A/A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.021
-
 sulfamethazine mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.023
-
 sulfadoxine mutant K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.025
-
 sulfathiazole mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.027
-
 sulfathiazole mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.03
-
 Pteroate mutant A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.034
-
 sulfamethoxazole wild-type, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.035
-
 sulfamonomethoxine mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.039
-
 Pteroate mutant A581G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.04
-
 dapsone mutant A581G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.04
-
 sulfadimethoxine mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.058
-
 dapsone mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.058
-
 sulfamethazine mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.07
-
 sulfadoxine mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.084
-
 sulfadoxine mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.086
-
 sulfadoxine mutant S436F/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.116
-
 sulfadimethoxine mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.124
-
 sulfadoxine mutant S436F/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.153
-
 sulfamethoxazole mutant A437G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.156
-
 dapsone mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.177
-
 dapsone mutant S436F/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.192
-
 dapsone mutant S436F/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.196
-
 sulfadoxine mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.198
-
 sulfadoxine mutant A581G, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.462
-
 sulfamethoxazole mutant S436A/A437G/A613S, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.48
-
 sulfamethoxazole mutant A437G/K540E, pH 9, 37°C Plasmodium falciparum
2.7.6.3 0.731
-
 sulfamethoxazole mutant S436A/A437G/A613T, pH 9, 37°C Plasmodium falciparum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.15 4.08
-
 4-Aminobenzoate mutant enzyme S436F/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 5.38
-
 4-Aminobenzoate mutant enzyme A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 6.45
-
 4-Aminobenzoate mutant enzyme S436F/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 6.55
-
 4-Aminobenzoate mutant enzyme A581G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 7.28
-
 4-Aminobenzoate mutant enzyme K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 9.13
-
 4-Aminobenzoate mutant enzyme A437G/K540E, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 9.9
-
 4-Aminobenzoate mutant enzyme A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 10.17
-
 4-Aminobenzoate wild type enzyme, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 10.35
-
 4-Aminobenzoate mutant enzyme S436F/A437G/A613T, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 16.67
-
 4-Aminobenzoate mutant enzyme S436F/A437G/A613S, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 21.43
-
 4-Aminobenzoate mutant enzyme S436A/A437G, at pH 9.0 and 37°C Plasmodium falciparum
2.5.1.15 60.6
-
 4-Aminobenzoate mutant enzyme A437G, at pH 9.0 and 37°C Plasmodium falciparum