EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.10 | hexahistidine-tagged octaprenyl diphosphate synthase | Escherichia coli |
2.5.1.90 | hexahistidine-tagged octaprenyl diphosphate synthase | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.90 | 0.0007 | - |
dimethylallyl diphosphate | at pH 7.5 and 30°C | Escherichia coli | |
2.5.1.90 | 0.34 | - |
isopentenyl diphosphate | with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 30°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.90 | Mg2+ | 1 mM used in assay conditions | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Escherichia coli | - |
5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | Escherichia coli K12 | - |
5 diphosphate + all-trans-octaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.10 | Escherichia coli | P22939 | K12 strain SF5 | - |
2.5.1.10 | Escherichia coli K12 | P22939 | K12 strain SF5 | - |
2.5.1.90 | Escherichia coli | P0AD57 | - |
- |
2.5.1.90 | Escherichia coli | P0AD57 | K12 strain SF5 | - |
2.5.1.90 | Escherichia coli K12 | P0AD57 | K12 strain SF5 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.10 | hexahistidine-tagged octaprenyl diphosphate synthase | Escherichia coli |
2.5.1.90 | hexahistidine-tagged octaprenyl diphosphate synthase | Escherichia coli |
2.5.1.90 | Ni-NTA agarose column chromatography | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.90 | 430 | - |
with dimethylallyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
2.5.1.90 | 3100 | - |
with geranyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
2.5.1.90 | 31000 | - |
with geranylgeranyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
2.5.1.90 | 58000 | - |
with (2E,6E)-farnesyl diphosphate as substrate, at pH 7.5 and 30°C | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.10 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.10 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli K12 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | (2E,6E)-farnesyl diphosphate is the most effective substrate | Escherichia coli | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | - |
Escherichia coli K12 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | (2E,6E)-farnesyl diphosphate + 5 isopentenyl diphosphate | (2E,6E)-farnesyl diphosphate is the most effective substrate | Escherichia coli K12 | 5 diphosphate + all-trans-octaprenyl diphosphate | - |
? | |
2.5.1.90 | dimethylallyl diphosphate + isopentenyl diphosphate | 0.7% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli | diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate | - |
? | |
2.5.1.90 | dimethylallyl diphosphate + isopentenyl diphosphate | 0.7% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli K12 | diphosphate + octaprenyl diphosphate + nonaprenyl diphosphate | - |
? | |
2.5.1.90 | geranyl diphosphate + isopentenyl diphosphate | 5.3% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli | ? | - |
? | |
2.5.1.90 | geranyl diphosphate + isopentenyl diphosphate | 5.3% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli K12 | ? | - |
? | |
2.5.1.90 | geranylgeranyl diphosphate + isopentenyl diphosphate | 53.4% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli | ? | - |
? | |
2.5.1.90 | geranylgeranyl diphosphate + isopentenyl diphosphate | 53.4% activity compared to (2E,6E)-farnesyl diphosphate | Escherichia coli K12 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.90 | ? | x * 37000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.10 | IspA | - |
Escherichia coli |
2.5.1.90 | IspB | - |
Escherichia coli |
2.5.1.90 | octaprenyl diphosphate synthase | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.10 | malfunction | slow growth of an Escherichia coli ispA null mutant lacking farnesyl diphosphate synthase. The growth rates of ispA mutant-transformants harboring a medium-copy number plasmid that carries ispA is almost the same as that of the wild-type strain | Escherichia coli |