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Literature summary extracted from

  • Motokawa, Y.; Kikuchi, G.
    Glycine metabolism by rat liver mitochondria. Reconstruction of the reversible glycine cleavage system with partially purified protein components (1974), Arch. Biochem. Biophys., 164, 624-633 .
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.27 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH Rattus norvegicus
-
 glycine + tetrahydrofolate + NAD+
-
 r
1.4.1.27 glycine + tetrahydrofolate + NAD+ Rattus norvegicus
-
 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.27 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.27 purification of P-protein, and for T-protein. The procedure leads to the isolation of a nearly homogeneous form of T-protein but P-protein still is heterogeneous Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.27 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
 Rattus norvegicus glycine + tetrahydrofolate + NAD+
-
 r
1.4.1.27 glycine + tetrahydrofolate + NAD+
-
 Rattus norvegicus 5,10-methylenetetrahydrofolate + NH3 + CO2 + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.27 multimer x * 33,000, component T-protein Rattus norvegicus

General Information

EC Number General Information Comment Organism
1.4.1.27 physiological function the reversible glycine cleavage system is composed of four protein components named as P-, H-, L-, and T-protein, respectively. P-protein catalyzes the decarboxylation of glycine or its reverse reaction in the presence of H-protein, and T-protein participates in the formation of one carbon unit and ammonia or the reverse reaction Rattus norvegicus