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Literature summary extracted from

  • Ni, W.; Zheng, Z.; Liu, H.; Wang, P.; Wang, H.; Sun, X.; Yang, Q.; Fang, Z.; Tang, H.; Zhao, G.
    Combining mutagenesis on Glu281 of prenyltransferase NovQ and metabolic engineering strategies for the increased prenylated activity towards menadione (2020), Appl. Microbiol. Biotechnol., 104, 4371-4382 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.111 expressed in Escherichia coli BL21(DE3) cells Streptomyces niveus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.111 E281A the mutant shows about 10% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281D the mutant shows about 78% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281K the mutant shows about 60% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281M the mutant shows about 98% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281Q the mutant shows 205% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281R the mutant shows about 5% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281S the mutant shows about 30% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281T the mutant shows about 40% activity compared to the wild type enzyme Streptomyces niveus
2.5.1.111 E281V the mutant shows about 78% activity compared to the wild type enzyme Streptomyces niveus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.111 menadione hydroquinol the relative activity is drastically decreased when the reaction system is supplemented with more than 1.5 mM of menadione hydroquinol. 3 mM menadione hydroquinol almost absolutely inhibits the prenylation by precipitating the enzyme in the reaction system Streptomyces niveus
2.5.1.111 additional information nearly full activity is obtained in the presence of 10 mM EDTA Streptomyces niveus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.111 0.1044
-
 menadione hydroquinol mutant enzyme E281R, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.1537
-
 menadione hydroquinol mutant enzyme E281Q, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.1737
-
 menadione hydroquinol wild type enzyme, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.2106
-
 menadione hydroquinol mutant enzyme E281M, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.2181
-
 menadione hydroquinol mutant enzyme E281K, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.2933
-
 menadione hydroquinol mutant enzyme E281V, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.3623
-
 menadione hydroquinol mutant enzyme E281A, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.4097
-
 menadione hydroquinol mutant enzyme E281S, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.4799
-
 menadione hydroquinol mutant enzyme E281T, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.5202
-
 menadione hydroquinol mutant enzyme E281D, at pH 8.0 and 32°C Streptomyces niveus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.111 Mg2+ 2.5 mM used in assay conditions Streptomyces niveus
2.5.1.111 additional information the enzyme is independent of metal ions Streptomyces niveus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces niveus
-
 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate Streptomyces niveus CPCC 240348
-
 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.111 Streptomyces niveus Q9L9F1
-
-
2.5.1.111 Streptomyces niveus CPCC 240348 Q9L9F1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
-
 Streptomyces niveus diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + 4-hydroxyphenylpyruvate
-
 Streptomyces niveus CPCC 240348 diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate
-
 ?
2.5.1.111 dimethylallyl diphosphate + menadione hydroquinol
-
 Streptomyces niveus diphosphate + vitamin MK1
-
 ?
2.5.1.111 dimethylallyl diphosphate + menadione hydroquinol
-
 Streptomyces niveus CPCC 240348 diphosphate + vitamin MK1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.111 NovQ
-
 Streptomyces niveus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.111 32
-
-
 Streptomyces niveus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.111 0.003
-
 menadione hydroquinol mutant enzyme E281R, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.005
-
 menadione hydroquinol mutant enzyme E281A, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.014
-
 menadione hydroquinol mutant enzyme E281S, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.022
-
 menadione hydroquinol mutant enzyme E281K, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.026
-
 menadione hydroquinol mutant enzyme E281T, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.038
-
 menadione hydroquinol mutant enzyme E281V, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.038
-
 menadione hydroquinol wild type enzyme, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.039
-
 menadione hydroquinol mutant enzyme E281M, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.045
-
 menadione hydroquinol mutant enzyme E281D, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.083
-
 menadione hydroquinol mutant enzyme E281Q, at pH 8.0 and 32°C Streptomyces niveus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.111 8
-
-
 Streptomyces niveus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.111 0.014
-
 menadione hydroquinol mutant enzyme E281A, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.029
-
 menadione hydroquinol mutant enzyme E281R, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.054
-
 menadione hydroquinol mutant enzyme E281T, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.064
-
 menadione hydroquinol mutant enzyme E281S, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.087
-
 menadione hydroquinol mutant enzyme E281D, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.1
-
 menadione hydroquinol mutant enzyme E281K, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.13
-
 menadione hydroquinol mutant enzyme E281V, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.185
-
 menadione hydroquinol mutant enzyme E281M, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.219
-
 menadione hydroquinol wild type enzyme, at pH 8.0 and 32°C Streptomyces niveus
2.5.1.111 0.54
-
 menadione hydroquinol mutant enzyme E281Q, at pH 8.0 and 32°C Streptomyces niveus