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Literature summary extracted from

  • Damasio, A.R.; Rubio, M.V.; Goncalves, T.A.; Persinoti, G.F.; Segato, F.; Prade, R.A.; Contesini, F.J.; de Souza, A.P.; Buckeridge, M.S.; Squina, F.M.
    Xyloglucan breakdown by endo-xyloglucanase family 74 from Aspergillus fumigatus (2017), Appl. Microbiol. Biotechnol., 101, 2893-2903 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.151 gene AFUA_8G01490, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, detailed overview. Recombinant enzyme expression in Aspergillus nidulans strain A773 Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.151 additional information
-
 additional information kinetics with substrate XyGt, pH 5.5, 50°C, Km is 1.5 mg/ml Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.151 xyloglucan + H2O Aspergillus fumigatus
-
 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O Aspergillus fumigatus CBS 101355
-
 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O Aspergillus fumigatus ATCC MYA-4609
-
 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O Aspergillus fumigatus FGSC A1100
-
 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O Aspergillus fumigatus Af293
-
 xyloglucan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.151 Aspergillus fumigatus Q4WB93 i.e. Aspergillus fumigatus
-
3.2.1.151 Aspergillus fumigatus Af293 Q4WB93 i.e. Aspergillus fumigatus
-
3.2.1.151 Aspergillus fumigatus ATCC MYA-4609 Q4WB93 i.e. Aspergillus fumigatus
-
3.2.1.151 Aspergillus fumigatus CBS 101355 Q4WB93 i.e. Aspergillus fumigatus
-
3.2.1.151 Aspergillus fumigatus FGSC A1100 Q4WB93 i.e. Aspergillus fumigatus
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.151 glycoprotein the enzyme has five predicted N-glycosylation sites Aspergillus fumigatus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.151 mycelium
-
 Aspergillus fumigatus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.151 11.9
-
 purified recombinant enzyme, pH 5.5, 50°C, substrate XyGt Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.151 xyloglucan + H2O
-
 Aspergillus fumigatus xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides Aspergillus fumigatus xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus fumigatus CBS 101355 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides Aspergillus fumigatus CBS 101355 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus fumigatus ATCC MYA-4609 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides Aspergillus fumigatus ATCC MYA-4609 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus fumigatus FGSC A1100 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides Aspergillus fumigatus FGSC A1100 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O
-
 Aspergillus fumigatus Af293 xyloglucan oligosaccharides
-
 ?
3.2.1.151 xyloglucan + H2O xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides Aspergillus fumigatus Af293 xyloglucan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.151 ? x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.151 AfXEG74
-
 Aspergillus fumigatus
3.2.1.151 AspGD
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.151 50
-
 recombinant enzyme, with substrate XyGt Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.151 30 65 activity range, recombinant enzyme, profile overview Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.151 57.2
-
 melting temperature (Tm) of AfXEG74 is 57.2°C Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.151 16.4
-
 xyloglucan recombinant enzyme, pH 5.5, 50°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.151 5.5
-
 recombinant enzyme, with substrate XyGt Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.151 4.5 7.5 activity range, recombinant enzyme, profile overview Aspergillus fumigatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.151 Aspergillus fumigatus sequence calculation
-
 5.11

General Information

EC Number General Information Comment Organism
3.2.1.151 evolution the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview Aspergillus fumigatus
3.2.1.151 additional information mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches Aspergillus fumigatus
3.2.1.151 physiological function enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides Aspergillus fumigatus