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Literature summary extracted from

  • Ko, T.P.; Xiao, X.; Guo, R.T.; Huang, J.W.; Liu, W.; Chen, C.C.
    Substrate-analogue complex structure of Mycobacterium tuberculosis decaprenyl diphosphate synthase (2019), Acta Crystallogr. Sect. F, 75, 212-216 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.86 expressed in Escherichia coli Mycobacterium tuberculosis
2.5.1.86 expression in Escherichia coli Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.86 enzyme in complex with the substrate analogues geranyl S-thiodiphosphate and isopentenyl S-thiodiphosphate, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 10% (v/v) glycerol, 25% (w/v) PEG 400, 7% (w/v) PEG 3000 Mycobacterium tuberculosis
2.5.1.86 sitting-drop vapour-diffusion method at 25°C. A 1.55 A resolution structure of the enzyme in complex with the substrate analogues geranyl S-thiodiphosphate and isopentenyl S-thiodiphosphate bound to their respective sites in one subunit shows the active-site configuration and the magnesium-coordinated geometry for catalysis Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.86 Mg2+ contains Mg2+ Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Mycobacterium tuberculosis
-
 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Mycobacterium tuberculosis H37Rv
-
 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate Mycobacterium tuberculosis ATCC 25618
-
 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.86 Mycobacterium tuberculosis P9WFF7
-
-
2.5.1.86 Mycobacterium tuberculosis ATCC 25618 P9WFF7
-
-
2.5.1.86 Mycobacterium tuberculosis H37Rv P9WFF7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.86 Ni-NTA column chromatography and HiPrep 16/60 S-100 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Mycobacterium tuberculosis 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Mycobacterium tuberculosis H37Rv 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?
2.5.1.86 (2Z,6E)-farnesyl diphosphate + 7 isopentenyl diphosphate
-
 Mycobacterium tuberculosis ATCC 25618 7 diphosphate + trans,octacis-decaprenyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.86 homodimer x-ray crystallography Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.86 decaprenyl diphosphate synthase
-
 Mycobacterium tuberculosis
2.5.1.86 DPPS
-
 Mycobacterium tuberculosis
2.5.1.86 MtDPPS
-
 Mycobacterium tuberculosis
2.5.1.86 Rv2361c
-
 Mycobacterium tuberculosis