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Literature summary extracted from

  • Mitachi, K.; Yun, H.; Gillman, C.; Skorupinska-Tudek, K.; Swiezewska, E.; Clemons, W.; Kurosu, M.
    Substrate tolerance of bacterial glycosyltransferase MurG novel fluorescence-based assays (2019), ACS Infect. Dis., 6, 1501-1516 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.227 the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid is transformed and expressed in Escherichia coli NiCo21(DE3) competent cells Mycolicibacterium smegmatis
2.4.1.227 the murG gene is cloned with an N-terminal His6 tag into a pET33b vector. The plasmid was transformed and expressed in Escherichia coli NiCo21(DE3) competent cells Hydrogenivirga sp. 128-5-R1-1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.227 nikkomycin Z
-
 Hydrogenivirga sp. 128-5-R1-1
2.4.1.227 nikkomycin Z
-
 Mycolicibacterium smegmatis
2.4.1.227 Polyoxin D
-
 Hydrogenivirga sp. 128-5-R1-1
2.4.1.227 Polyoxin D
-
 Mycolicibacterium smegmatis
2.4.1.227 PyDT-1
-
 Mycolicibacterium smegmatis
2.4.1.227 ramoplanin A2
-
 Hydrogenivirga sp. 128-5-R1-1
2.4.1.227 ramoplanin A2
-
 Mycolicibacterium smegmatis
2.4.1.227 ristocetin A strong antimycobacterial MurG inhibitor Hydrogenivirga sp. 128-5-R1-1
2.4.1.227 ristocetin A strong antimycobacterial MurG inhibitor Mycolicibacterium smegmatis
2.4.1.227 ToXa-1
-
 Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.227 Hydrogenivirga sp. 128-5-R1-1 A8US35
-
-
2.4.1.227 Mycolicibacterium smegmatis
-
-
-
2.4.1.227 Mycolicibacterium smegmatis ATCC 607
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.227
-
 Mycolicibacterium smegmatis
2.4.1.227
-
 Hydrogenivirga sp. 128-5-R1-1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.227 additional information
-
 fluorescence-based assay Mycolicibacterium smegmatis
2.4.1.227 additional information
-
 fluorescence-based assay Hydrogenivirga sp. 128-5-R1-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.227 UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea
-
 Mycolicibacterium smegmatis UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea
-
 ?
2.4.1.227 UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea
-
 Hydrogenivirga sp. 128-5-R1-1 UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea
-
 ?
2.4.1.227 UDP-N-acetyl-alpha-D-glucosamine + C55-lipid I-Nepsilon-C6-dansyl thiourea
-
 Mycolicibacterium smegmatis ATCC 607 UDP + C55-lipid II-Nepsilon-C6-dansyl thiourea
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.227 HyMurG
-
 Hydrogenivirga sp. 128-5-R1-1
2.4.1.227 MsmegMurG
-
 Mycolicibacterium smegmatis
2.4.1.227 MurG
-
 Mycolicibacterium smegmatis
2.4.1.227 MurG
-
 Hydrogenivirga sp. 128-5-R1-1

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.227 0.00096
-
 pH 8.0, 37°C Hydrogenivirga sp. 128-5-R1-1 ristocetin A
2.4.1.227 0.0014
-
 pH 8.0, 37°C Mycolicibacterium smegmatis ristocetin A
2.4.1.227 0.002
-
 pH 8.0, 37°C Mycolicibacterium smegmatis ToXa-1
2.4.1.227 0.0035
-
 pH 8.0, 37°C Mycolicibacterium smegmatis PyDT-1
2.4.1.227 0.0087
-
 pH 8.0, 37°C Hydrogenivirga sp. 128-5-R1-1 nikkomycin Z
2.4.1.227 0.0095
-
 pH 8.0, 37°C Mycolicibacterium smegmatis nikkomycin Z
2.4.1.227 0.0224
-
 pH 8.0, 37°C Hydrogenivirga sp. 128-5-R1-1 ramoplanin A2
2.4.1.227 0.0235
-
 pH 8.0, 37°C Mycolicibacterium smegmatis ramoplanin A2
2.4.1.227 0.0508
-
 pH 8.0, 37°C Hydrogenivirga sp. 128-5-R1-1 Polyoxin D
2.4.1.227 0.055
-
 pH 8.0, 37°C Mycolicibacterium smegmatis Polyoxin D

General Information

EC Number General Information Comment Organism
2.4.1.227 metabolism the enzyme is an essential bacterial glycosyltransferase that catalyses the GlcNAc-transformation of lipid I to lipid II during peptidoglycan biosynthesis Mycolicibacterium smegmatis
2.4.1.227 metabolism the enzyme is an essential bacterial glycosyltransferase that catalyses the GlcNAc-transformation of lipid I to lipid II during peptidoglycan biosynthesis Hydrogenivirga sp. 128-5-R1-1