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Literature summary extracted from

  • Samori, P.Y.; Makabe, K.; Ohya, N.; Hatano, B.; Murakami, S.; Kijima, T.
    Role of Cys73 in the thermostability of farnesyl diphosphate synthase from Geobacillus stearothermophilus (2017), 3 Biotech, 7, 236 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.92 expression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.10 sitting-drop vapor diffusion method, crystal structure of FPPase from Geobacillus stearothermophilus is determined at 2.31 A resolution Geobacillus stearothermophilus
2.5.1.92 sitting-drop vapor diffusion method, the crystal structure is determined at 2.31 A resolution. The structure of GsFPPase shows a three-layered all alpha-helical fold and conserved functional domains similar to other prenyltransferases Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.10 C73F thermostability of the mutant enzyme C73F is lower than that of the wild-type enzyme, but higher when compared with human enzyme Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Geobacillus stearothermophilus
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.92 dimethylallyl diphosphate + 2 isopentenyl diphosphate Geobacillus stearothermophilus the product farnesyl diphosphate is an important precursor in the isoprenoid synthesis pathway 2 diphosphate + (2Z,6Z)-farnesyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Geobacillus stearothermophilus Q08291
-
-
2.5.1.92 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.10
-
 Geobacillus stearothermophilus
2.5.1.92
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Geobacillus stearothermophilus diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.92 dimethylallyl diphosphate + 2 isopentenyl diphosphate
-
 Geobacillus stearothermophilus 2 diphosphate + (2Z,6Z)-farnesyl diphosphate
-
 ?
2.5.1.92 dimethylallyl diphosphate + 2 isopentenyl diphosphate the product farnesyl diphosphate is an important precursor in the isoprenoid synthesis pathway Geobacillus stearothermophilus 2 diphosphate + (2Z,6Z)-farnesyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 FPPase
-
 Geobacillus stearothermophilus
2.5.1.92 FPPase
-
 Geobacillus stearothermophilus