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Literature summary extracted from

  • Garefalaki, V.; Kontomina, E.; Ioannidis, C.; Savvidou, O.; Vagena-Pantoula, C.; Papavergi, M.G.; Olbasalis, I.; Patriarcheas, D.; Fylaktakidou, K.C.; Felfoeldi, T.; Marialigeti, K.; Fakis, G.; Boukouvala, S.
    The actinobacterium Tsukamurella paurometabola has a functionally divergent arylamine N-acetyltransferase (NAT) homolog (2019), World J. Microbiol. Biotechnol., 35, 174 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.5 expresssion in Escherichia coli Tsukamurella paurometabola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.5 0.486
-
 malonyl-CoA cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola
2.3.1.5 2.222
-
 acetyl-CoA cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.5 Tsukamurella paurometabola D5UNC2
-
-
2.3.1.5 Tsukamurella paurometabola DSM 20162 D5UNC2
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.5 1.47
-
 donor succinyl-CoA, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola
2.3.1.5 1.908
-
 donor acetyl-CoA, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola
2.3.1.5 2.012
-
 donor propionyl-CoA, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola
2.3.1.5 3.53
-
 donor malonyl-CoA, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.5 acetyl-CoA + 3,4-dichloroaniline
-
 Tsukamurella paurometabola CoA + N-(3,4-dichlorophenyl)acetamide
-
 ?
2.3.1.5 acetyl-CoA + 3,4-dichloroaniline
-
 Tsukamurella paurometabola DSM 20162 CoA + N-(3,4-dichlorophenyl)acetamide
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylate
-
 Tsukamurella paurometabola CoA + N-acetyl-5-aminosalicylate
-
 ?
2.3.1.5 acetyl-CoA + 5-aminosalicylate
-
 Tsukamurella paurometabola DSM 20162 CoA + N-acetyl-5-aminosalicylate
-
 ?
2.3.1.5 malonyl-CoA + 2-aminophenol
-
 Tsukamurella paurometabola CoA + N-malonyl-2-aminophenol
-
 ?
2.3.1.5 malonyl-CoA + 2-aminophenol
-
 Tsukamurella paurometabola DSM 20162 CoA + N-malonyl-2-aminophenol
-
 ?
2.3.1.5 malonyl-CoA + 3,4-dichloroaniline
-
 Tsukamurella paurometabola CoA + 3-(3,4-dichloroanilino)-3-oxopropanoic acid
-
 ?
2.3.1.5 malonyl-CoA + 3,4-dichloroaniline
-
 Tsukamurella paurometabola DSM 20162 CoA + 3-(3,4-dichloroanilino)-3-oxopropanoic acid
-
 ?
2.3.1.5 malonyl-CoA + 4-aminobenzoate
-
 Tsukamurella paurometabola CoA + N-malonyl-4-aminobenzoate
-
 ?
2.3.1.5 malonyl-CoA + 4-aminobenzoate
-
 Tsukamurella paurometabola DSM 20162 CoA + N-malonyl-4-aminobenzoate
-
 ?
2.3.1.5 malonyl-CoA + 5-aminosalicylate malonyl-CoA again is the preferred donor substrate Tsukamurella paurometabola CoA + N-malonyl-5-aminosalicylate
-
 ?
2.3.1.5 propionyl-CoA + 5-aminosalicylate
-
 Tsukamurella paurometabola CoA + N-propionyl-5-aminosalicylate
-
 ?
2.3.1.5 succinyl-CoA + 5-aminosalicylate
-
 Tsukamurella paurometabola CoA + N-succinyl-5-aminosalicylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.5 ? x * 31200, calculated from sequence Tsukamurella paurometabola

Synonyms

EC Number Synonyms Comment Organism
2.3.1.5 Tpau_4046
-
 Tsukamurella paurometabola

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.5 9.7
-
 malonyl-CoA cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola
2.3.1.5 19.2
-
 acetyl-CoA cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.5 8.416
-
 acetyl-CoA cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola
2.3.1.5 22.398
-
 malonyl-CoA cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication Tsukamurella paurometabola