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Literature summary extracted from
- Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures (2018), Sci. Rep., 8, 1454 .
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.8.1.9 | crystals are obtained by hanging-drop vapour diffusion method. The enzyme is crystallized in the presence of the substrate D-2-chloropropionate at pH 5.5-6.4 and 4°C. 2.64 A resolution | Pseudomonas putida |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.8.1.9 | D205E | activity with D-2-chloropropionate is 10.1% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 13fold higher compared to mutant enzyme | Pseudomonas putida |
| 3.8.1.9 | D205N | no activity with D-2-chloropropionate | Pseudomonas putida |
| 3.8.1.9 | F281A | activity with D-2-chloropropionate is 54.7% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | G50A | activity with D-2-chloropropionate is 5.1% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | I52G | activity with D-2-chloropropionate is 1.5% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | L285I | activity with D-2-chloropropionate is 24.4% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | L288I | activity with D-2-chloropropionate is 37.2% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | M284C | activity with D-2-chloropropionate is 7.0% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | N131D | activity with D-2-chloropropionate is 4.4% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | N203A | activity with D-2-chloropropionate is 1.2% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 355fold higher compared to mutant enzyme | Pseudomonas putida |
| 3.8.1.9 | N203S | activity with D-2-chloropropionate is 0.4% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | S204A | activity with D-2-chloropropionate is 4.9% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | S204T | activity with D-2-chloropropionate is 41.8% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | V51F | activity with D-2-chloropropionate is 36.6% compared to activity of the wild-type enzyme. The kcat value of wild-type enzyme is 4fold higher compared to mutant enzyme | Pseudomonas putida |
| 3.8.1.9 | W48A | activity with D-2-chloropropionate is 0.2% compared to activity of the wild-type enzyme | Pseudomonas putida |
| 3.8.1.9 | Y134F | activity with D-2-chloropropionate is 0.1% compared to activity of the wild-type enzyme | Pseudomonas putida |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.9 | 0.94 | - |
D-2-chloropropionate | wild-type enzyme, pH 10.0, 30°C | Pseudomonas putida |  |
| 3.8.1.9 | 2.9 | - |
D-2-chloropropionate | mutant enzyme V51F, pH 10.0, 30°C | Pseudomonas putida | |
| 3.8.1.9 | 4.13 | - |
D-2-chloropropionate | mutant enzyme D205E, pH 10.0, 30°C | Pseudomonas putida | |
| 3.8.1.9 | 12 | - |
D-2-chloropropionate | mutant enzyme N203A, pH 10.0, 30°C | Pseudomonas putida | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.8.1.9 | Pseudomonas putida | Q52086 | - |
- |
| 3.8.1.9 | Pseudomonas putida AJ1/23 | Q52086 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.8.1.9 | D-2-chloropropionate + H2O | - |
Pseudomonas putida | L-2-hydroxypropionate + chloride | - |
? | |
| 3.8.1.9 | D-2-chloropropionate + H2O | - |
Pseudomonas putida AJ1/23 | L-2-hydroxypropionate + chloride | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.8.1.9 | homotetramer | - |
Pseudomonas putida |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.8.1.9 | D-2-haloacid dehalogenase | - |
Pseudomonas putida |
| 3.8.1.9 | D-DEX | - |
Pseudomonas putida |
| 3.8.1.9 | HadD AJ1 | - |
Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.8.1.9 | 0.59 | - |
D-2-chloropropionate | mutant enzyme N203A, pH 10.0, 30°C | Pseudomonas putida | |
| 3.8.1.9 | 12.6 | - |
D-2-chloropropionate | mutant enzyme V51F, pH 10.0, 30°C | Pseudomonas putida | |
| 3.8.1.9 | 16.35 | - |
D-2-chloropropionate | wild-type enzyme, pH 10.0, 30°C | Pseudomonas putida | |
| 3.8.1.9 | 55 | - |
D-2-chloropropionate | mutant enzyme D205E, pH 10.0, 30°C | Pseudomonas putida | |
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Release 2023.1 (January 2023)
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