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Literature summary extracted from

  • Neubauer, P.R.; Widmann, C.; Wibberg, D.; Schroeder, L.; Frese, M.; Kottke, T.; Kalinowski, J.; Niemann, H.H.; Sewald, N.
    A flavin-dependent halogenase from metagenomic analysis prefers bromination over chlorination (2018), PLoS ONE, 13, e0196797 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.B10 expressed in Escherichia coli BL21(DE3) cells Brevundimonas sp. BAL3

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.19.B10 using 1.0 M Na/K tartrate, 0.1 M MES pH 6.0 Brevundimonas sp. BAL3

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.B10 Brevundimonas sp. BAL3 B4WBL8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.B10 His-Talon column chromatography, Ni2+-affinity chromatography, Source 15 Q column chromatography, and Superdex S200 gel filtration Brevundimonas sp. BAL3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.B10 indole + FADH2 + bromide + O2 + H+
-
 Brevundimonas sp. BAL3 3-bromoindole + FAD + 2 H2O
-
 ?
1.14.19.B10 indole + FADH2 + chloride + O2 + H+ low activity Brevundimonas sp. BAL3 3-chloroindole + FAD + 2 H2O
-
 ?
1.14.19.B10 additional information the enzyme prefers bromide to chloride as halide source. The enzyme shows no halogenation of tryptophan, neither in presence of chloride nor in presence of bromide and has no activity with 5-hydroxytryptohan, tryptophol, indole-3-propionic acid, and indole-3-acetonitrile Brevundimonas sp. BAL3 ?
-
-

Synonyms

EC Number Synonyms Comment Organism
1.14.19.B10 brvH
-
 Brevundimonas sp. BAL3
1.14.19.B10 FHal
-
 Brevundimonas sp. BAL3
1.14.19.B10 flavin-dependent halogenase
-
 Brevundimonas sp. BAL3

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.B10 FADH2
-
 Brevundimonas sp. BAL3
1.14.19.B10 NAD+
-
 Brevundimonas sp. BAL3