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Literature summary extracted from

  • Ciarkowska, A.; Ostrowski, M.; Jakubowska, A.
    A serine carboxypeptidase-like acyltransferase catalyzes synthesis of indole-3-acetic (IAA) ester conjugate in rice (Oryza sativa) (2018), Plant Physiol. Biochem., 125, 126-135 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.72 expressed in Saccharomyces cerevisiae strain InvSc1 and Escherichia coli strain BL21-CodonPlus(DE3)-RIL Oryza sativa Japonica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.72 phenylmethylsulfonyl fluoride
-
 Oryza sativa Japonica Group

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.72 0.83
-
 myo-inositol recombinant enzyme from Saccharomyces cerevisiae, at pH 7.4 and 30°C Oryza sativa Japonica Group
2.3.1.72 1
-
 myo-inositol recombinant enzyme from Escherichia coli, at pH 7.4 and 30°C Oryza sativa Japonica Group
2.3.1.72 1.38
-
 myo-inositol native enzyme, at pH 7.4 and 30°C Oryza sativa Japonica Group

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.72 1-O-(indol-3-yl)acetyl-beta-D-glucose + myo-inositol Oryza sativa Japonica Group
-
 D-glucose + O-(indol-3-yl)acetyl-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.72 Oryza sativa Japonica Group B9EZV5
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.72 glycoprotein glycosylation is not necessary for enzyme activity Oryza sativa Japonica Group

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.72 ConA-agarose column chromatography Oryza sativa Japonica Group

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.3.1.72 recombinant enzyme from Escherichia coli, using 50 mM Tris-HCl buffer pH 8.5 containing 9.6 mM NaCl, 0.4 mM KCl, 2 mM MgCl2, 2 mM CaCl2, 0.5M arginin, 0.4 M sucrose, 0.75 mM guanidine hydrochloride, 1 mM GSH and 0.1 mM GSSH Oryza sativa Japonica Group

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.72 seedling
-
 Oryza sativa Japonica Group
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.72 0.00501
-
 native enzyme, at pH 7.4 and 30°C Oryza sativa Japonica Group
2.3.1.72 0.01809
-
 recombinant enzyme from Escherichia coli, at pH 7.4 and 30°C Oryza sativa Japonica Group
2.3.1.72 0.01875
-
 recombinant enzyme from Saccharomyces cerevisiae, at pH 7.4 and 30°C Oryza sativa Japonica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.72 1-O-(indol-3-yl)acetyl-beta-D-glucose + myo-inositol
-
 Oryza sativa Japonica Group D-glucose + O-(indol-3-yl)acetyl-myo-inositol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.72 ? x * 54300, native enzyme, calculated from amino acid sequence Oryza sativa Japonica Group
2.3.1.72 ? x * 54500, recombinant enzyme from Saccharomyces cerevisiae, calculated from amino acid sequence Oryza sativa Japonica Group
2.3.1.72 ? x * 58000, recombinant enzyme from Escherichia coli, calculated from amino acid sequence Oryza sativa Japonica Group
2.3.1.72 ? x * 58000, recombinant enzyme from Escherichia coli, SDS-PAGE Oryza sativa Japonica Group
2.3.1.72 ? x * 71000, native enzyme or recombinant enzyme from Saccharomyces cerevisiae, SDS-PAGE Oryza sativa Japonica Group

Synonyms

EC Number Synonyms Comment Organism
2.3.1.72 1-O-indole-3-acetyl-beta-D-glucose: myo-inositol acyltransferase
-
 Oryza sativa Japonica Group
2.3.1.72 IAA-At
-
 Oryza sativa Japonica Group
2.3.1.72 IAInos synthase
-
 Oryza sativa Japonica Group

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.72 7.4
-
-
 Oryza sativa Japonica Group

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.72 Oryza sativa Japonica Group native enzyme, calculated from amino acid sequence
-
 5.5
2.3.1.72 Oryza sativa Japonica Group recombinant enzyme from Saccharomyces cerevisiae, calculated from amino acid sequence
-
 5.5
2.3.1.72 Oryza sativa Japonica Group recombinant enzyme from Escherichia coli, calculated from amino acid sequence
-
 6

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.72 2.2
-
 recombinant enzyme from Escherichia coli, in the presence of 4.4 mM myo-inositol, at pH 7.4 and 30°C Oryza sativa Japonica Group phenylmethylsulfonyl fluoride
2.3.1.72 2.5
-
 recombinant enzyme from Saccharomyces cerevisiae, in the presence of 4.4 mM myo-inositol, at pH 7.4 and 30°C Oryza sativa Japonica Group phenylmethylsulfonyl fluoride
2.3.1.72 3.7
-
 native enzyme, in the presence of 4.4 mM myo-inositol, at pH 7.4 and 30°C Oryza sativa Japonica Group phenylmethylsulfonyl fluoride
2.3.1.72 4
-
 recombinant enzyme from Escherichia coli, in the presence of 8.1 mM myo-inositol, at pH 7.4 and 30°C Oryza sativa Japonica Group phenylmethylsulfonyl fluoride
2.3.1.72 4
-
 recombinant enzyme from Saccharomyces cerevisiae, in the presence of 8.1 mM myo-inositol, at pH 7.4 and 30°C Oryza sativa Japonica Group phenylmethylsulfonyl fluoride
2.3.1.72 6.7
-
 native enzyme, in the presence of 8.1 mM myo-inositol, at pH 7.4 and 30°C Oryza sativa Japonica Group phenylmethylsulfonyl fluoride