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Literature summary extracted from

  • Murata, K.; Kitano, T.; Yoshimoto, R.; Takata, R.; Ube, N.; Ueno, K.; Ueno, M.; Yabuta, Y.; Teraishi, M.; Holland, C.; Jander, G.; Okumoto, Y.; Mori, N.; Ishihara, A.
    Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influences antifungal defenses in diverse rice cultivars (2019), Plant J., 101, 1103-1117 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.232 agriculture NOMT expression and NOMT enzymatic activity are lower in cultivar Kasalath than in Nipponbare. A proline to threonine substitution in Kasalath relative to Nipponbare NOMT is the main cause of the lower enzymatic activity. NOMT induction is correlated with product sakuranetin accumulation. Substrate naringenin is more effective against bacterial pathogens and product sakuranetin is more effective against fungal pathogens Oryza sativa Japonica Group

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.232 expression in Escherichia coli Oryza sativa

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.232 additional information domain swapping experiments between Nipponbare and Kasalath NOMT genes. The replacement of the Kasalath O-methyltransferase domain with the Nipponbare domain increases the enzyme activity to the level of the Nipponbare enzyme Oryza sativa
2.1.1.232 P132T substitution in the Nipponbare background, results in a substantial decrease in the enzyme activity Oryza sativa Japonica Group
2.1.1.232 T130P mutation in cultivar Kassalth gene to match the corresonding residue in cultivar Nipponbare. Mutant shows activity comparable to Nipponbare NOMT Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.232 1.15
-
 (2S)-naringenin cultivar Kassalath, pH 9.5, 30°C Oryza sativa
2.1.1.232 1.15
-
 (2S)-naringenin cultivar Kasalath, pH 9.4, 30°C Oryza sativa Japonica Group
2.1.1.232 1.38
-
 (2S)-naringenin cultivar Nipponbare, pH 9.5, 30°c Oryza sativa
2.1.1.232 1.38
-
 (2S)-naringenin cultivar Nipponbare, pH 9.4, 30°C Oryza sativa Japonica Group
2.1.1.232 4.27
-
 S-adenosyl-L-methionine cultivar Nipponbare, pH 9.5, 30°c Oryza sativa
2.1.1.232 4.27
-
 S-adenosyl-L-methionine cultivar Nipponbare, pH 9.4, 30°C Oryza sativa Japonica Group
2.1.1.232 19.3
-
 S-adenosyl-L-methionine cultivar Kassalath, pH 9.5, 30°C Oryza sativa
2.1.1.232 19.3
-
 S-adenosyl-L-methionine cultivar Kasalath, pH 9.4, 30°C Oryza sativa Japonica Group

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.232 Oryza sativa
-
-
-
2.1.1.232 Oryza sativa Japonica Group Q0IP69
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.232 leaf
-
 Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin
-
 Oryza sativa S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?
2.1.1.232 S-adenosyl-L-methionine + (2S)-naringenin
-
 Oryza sativa Japonica Group S-adenosyl-L-homocysteine + (2S)-sakuranetin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.232 NOMT
-
 Oryza sativa Japonica Group
2.1.1.232 Os12g0240900
-
 Oryza sativa Japonica Group

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.232 3.03
-
 S-adenosyl-L-methionine cultivar Kassalath, pH 9.5, 30°C Oryza sativa
2.1.1.232 3.03
-
 (2S)-naringenin cultivar Kasalath, pH 9.4, 30°C Oryza sativa Japonica Group
2.1.1.232 26
-
 S-adenosyl-L-methionine cultivar Nipponbare, pH 9.5, 30°C Oryza sativa
2.1.1.232 26
-
 (2S)-naringenin cultivar Nipponbare, pH 9.4, 30°C Oryza sativa Japonica Group

General Information

EC Number General Information Comment Organism
2.1.1.232 evolution NOMT genes from low-sakuranetin-accumulating cultivars form at least two clusters. The low sakuranetin chemotype was acquired more than once in cultivated rice Oryza sativa Japonica Group
2.1.1.232 physiological function leaves of rice cultivar Nipponbare predominantly accumulate the phytoalexin sakuranetin after jasmonic acid induction. The Kasalath cultivar accumulates only low amounts of sakuranetin. Both NOMT expression and NOMT enzymatic activity are lower in Kasalath than in Nipponbare. A proline to threonine substitution in Kasalath relative to Nipponbare NOMT is the main cause of the lower enzymatic activity. The relative amounts of naringenin and sakuranetin may provide protection against specific pathogen profiles in different rice-growing environments. Naringenin is more effective against bacterial pathogens and sakuranetin is more effective against fungal pathogens Oryza sativa