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Literature summary extracted from

  • Xiang, X.; Wu, Y.; Planta, J.; Messing, J.; Leustek, T.
    Overexpression of serine acetyltransferase in maize leaves increases seed-specific methionine-rich zeins (2018), Plant Biotechnol. J., 16, 1057-1067 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.30 gene cysE, recombinant overexpression of Arabidopsis thaliana AtSAT1 in Zea mays leaves lines OE1 and OE3 under control of the leaf bundle sheath cell-specific rbcS1 promoter resulting in 12fold higher SAT activity, increased S-assimilation in the leaves, and higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. Quantitative RT-PCR expression analysis, the measured enzyme activity is a combination of endogenous SAT and that derived from expression of AtSAT1 Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.30 additional information overexpression of Arabidopsis thaliana AtSAT1 in maize under control of the leaf bundle sheath cell-specific rbcS1 promoter to determine the impact on seed storage protein expression. The transgenic events exhibit up to 12fold higher SAT activity without negative impact on growth. S-assimilation is increased in the leaves of SAT overexpressing plants, followed by higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. This zein is known to impact the level of Met stored in kernels. The elite event with the highest expression of AtSAT1 shows 1.40fold increase in kernel Met. Efficacy of increasing maize nutritional value by SAT overexpression without apparent yield loss. Maternal overexpression of SAT in vegetative tissues is necessary for high-Met zein accumulation. Moreover, SAT overcomes the shortage of S-amino acids that limits the expression and accumulation of high-Met zeins during kernel development. Mature kernels of AtSAT1 overexpressing maize show changes in zein expression profile. The measured enzyme activity is a combination of endogenous SAT and that derived from expression of AtSAT1 Zea mays
2.3.1.30 additional information the transgenic events in Zea mays leaves exhibit up to 12fold higher SAT activity without negative impact on growth. S-assimilation is increased in the leaves of SAT overexpressing plants, followed by higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. This zein is known to impact the level of Met stored in kernels. The elite event with the highest expression of AtSAT1 shows 1.40fold increase in kernel Met Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.30 cytosol AtSAT1 protein lacks a transit peptide Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine Arabidopsis thaliana
-
 CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine Zea mays
-
 CoA + O-acetyl-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.30 Arabidopsis thaliana Q42588
-
-
2.3.1.30 Zea mays A0A3L6DLJ7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.30 kernel
-
 Zea mays
-
2.3.1.30 leaf
-
 Arabidopsis thaliana
-
2.3.1.30 leaf
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.30 acetyl-CoA + L-serine
-
 Arabidopsis thaliana CoA + O-acetyl-L-serine
-
 ?
2.3.1.30 acetyl-CoA + L-serine
-
 Zea mays CoA + O-acetyl-L-serine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.30 AtSAT1
-
 Arabidopsis thaliana
2.3.1.30 SAT
-
 Arabidopsis thaliana
2.3.1.30 SAT
-
 Zea mays
2.3.1.30 SAT1
-
 Arabidopsis thaliana
2.3.1.30 SAT1
-
 Zea mays
2.3.1.30 serine acetyltransferase
-
 Arabidopsis thaliana
2.3.1.30 serine acetyltransferase
-
 Zea mays
2.3.1.30 serine acetyltransferase 1
-
 Arabidopsis thaliana
2.3.1.30 serine acetyltransferase 1
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.30 acetyl-CoA
-
 Arabidopsis thaliana
2.3.1.30 acetyl-CoA
-
 Zea mays

General Information

EC Number General Information Comment Organism
2.3.1.30 malfunction overexpression of Arabidopsis thaliana serine acetyltransferase in maize leaves increases seed-specific methionine-rich zeins, overexpression has the effect of, not only enhancing S-assimilation, but also, indirectly impacting expression of high-Met seed storage proteins Zea mays
2.3.1.30 metabolism serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis Arabidopsis thaliana
2.3.1.30 metabolism serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis. Met synthesis in maize is not strictly controlled by the enzyme cystathionine gamma-synthase (CGS) Zea mays
2.3.1.30 physiological function serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis Arabidopsis thaliana
2.3.1.30 physiological function serine acetyltransferase (SAT) is a key control point for S-assimilation leading to Cys and Met biosynthesis. SAT overexpression is known to enhance S-assimilation without negative impact on plant growth, it increases seed-specific methionine-rich D-zeins Zea mays