EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.1.376 | enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol | Homo sapiens |
2.4.2.63 | enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.376 | UDP-2-deoxy-2-fluoro-glucose | - |
Homo sapiens | |
2.4.2.63 | UDP-2-deoxy-2-fluoro-glucose | - |
Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.376 | UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine | Homo sapiens | the enzyme can only glycosylate serine residues in the C1XSXPC2 motif | UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine | - |
? | |
2.4.2.63 | additional information | Homo sapiens | the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates | ? | - |
- |
|
2.4.2.63 | UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine | Homo sapiens | - |
UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine | - |
? | |
2.4.2.63 | UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine | Homo sapiens | - |
UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.376 | Homo sapiens | Q8NBL1 | - |
- |
2.4.2.63 | Homo sapiens | Q8NBL1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.376 | IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
2.4.2.63 | IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.376 | UDP-alpha-D-glucose + [factor VII protein with EGF-like domain 1]-L-serine | - |
Homo sapiens | UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-glucosyl)-L-serine | - |
? | |
2.4.1.376 | UDP-alpha-D-glucose + [Notch1 protein with EGF-like domain 12]-L-serine | the enzyme can only glycosylate serine residues in the C1XSXPC2 motif | Homo sapiens | UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-glucosyl)-L-serine | - |
? | |
2.4.1.376 | UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine | the enzyme can only glycosylate serine residues in the C1XSXPC2 motif | Homo sapiens | UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine | - |
? | |
2.4.2.63 | additional information | the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates | Homo sapiens | ? | - |
- |
|
2.4.2.63 | UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine | - |
Homo sapiens | UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine | - |
? | |
2.4.2.63 | UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine | - |
Homo sapiens | UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.376 | POGLUT1 | - |
Homo sapiens |
2.4.1.376 | protein-O-glucosyltransferase 1 | - |
Homo sapiens |
2.4.1.376 | Rumi | - |
Homo sapiens |
2.4.2.63 | POGLUT1 | - |
Homo sapiens |
2.4.2.63 | protein-O-glucosyltransferase 1 | - |
Homo sapiens |
2.4.2.63 | Rumi | - |
Homo sapiens |