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Literature summary extracted from

  • Li, Z.; Fischer, M.; Satkunarajah, M.; Zhou, D.; Withers, S.; Rini, J.
    Structural basis of Notch O-glucosylation and O-xylosylation by mammalian protein-O-glucosyltransferase 1 (POGLUT1) (2017), Nat. Commun., 8, 185 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.376 enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol Homo sapiens
2.4.2.63 enzyme in complexes with three different EGF-like domains and either UDP, UDP-2-deoxy-2-fluoro-glucose or UDP-methyl-glucose, hanging drop vapor diffusion method, using 10-20% (w/v) PEG 5000 MME, 50 mM MES pH 6.5, 2-10 mM CaCl2, 0-250 mM NaCl, 5-10% (v/v) glycerol or 2-methyl-2,4-pentanediol Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.376 UDP-2-deoxy-2-fluoro-glucose
-
Homo sapiens
2.4.2.63 UDP-2-deoxy-2-fluoro-glucose
-
Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.376 UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine Homo sapiens the enzyme can only glycosylate serine residues in the C1XSXPC2 motif UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
?
2.4.2.63 additional information Homo sapiens the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates ?
-
-
2.4.2.63 UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine Homo sapiens
-
UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine
-
?
2.4.2.63 UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine Homo sapiens
-
UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.376 Homo sapiens Q8NBL1
-
-
2.4.2.63 Homo sapiens Q8NBL1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.376 IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens
2.4.2.63 IgG Sepharose affinity chromatography, HiTrap Sepharose column chromatography, and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.376 UDP-alpha-D-glucose + [factor VII protein with EGF-like domain 1]-L-serine
-
Homo sapiens UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-glucosyl)-L-serine
-
?
2.4.1.376 UDP-alpha-D-glucose + [Notch1 protein with EGF-like domain 12]-L-serine the enzyme can only glycosylate serine residues in the C1XSXPC2 motif Homo sapiens UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-glucosyl)-L-serine
-
?
2.4.1.376 UDP-alpha-D-glucose + [protein with EGF-like domain]-L-serine the enzyme can only glycosylate serine residues in the C1XSXPC2 motif Homo sapiens UDP + [protein with EGF-like domain]-3-O-(beta-D-glucosyl)-L-serine
-
?
2.4.2.63 additional information the enzyme does not require a diserine-containing glycosylation motif to efficiently xylosylate its substrates Homo sapiens ?
-
-
2.4.2.63 UDP-alpha-D-xylose + [factor VII protein with EGF-like domain 1]-L-serine
-
Homo sapiens UDP + [factor VII protein with EGF-like domain 1]-3-O-(beta-D-xylosyl)-L-serine
-
?
2.4.2.63 UDP-alpha-D-xylose + [Notch1 protein with EGF-like domain 12]-L-serine
-
Homo sapiens UDP + [Notch1 protein with EGF-like domain 12]-3-O-(beta-D-xylosyl)-L-serine
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.376 POGLUT1
-
Homo sapiens
2.4.1.376 protein-O-glucosyltransferase 1
-
Homo sapiens
2.4.1.376 Rumi
-
Homo sapiens
2.4.2.63 POGLUT1
-
Homo sapiens
2.4.2.63 protein-O-glucosyltransferase 1
-
Homo sapiens
2.4.2.63 Rumi
-
Homo sapiens