EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.273 | gene BSMT, recombinant expression of 35S::PbBSMT in planta in Arabidopsis thaliana reduces salicylate (SA) levels and increases susceptibility to clubroot, Plasmodiophora brassicae | Plasmodiophora brassicae |
2.1.1.274 | gene BSMT, recombinant expression of 35S::PbBSMT in planta in Arabidopsis thaliana reduces salicylate (SA) levels and increases susceptibility to clubroot, Plasmodiophora brassicae | Plasmodiophora brassicae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.273 | additional information | an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout | Arabidopsis thaliana |
2.1.1.273 | additional information | an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout | Plasmodiophora brassicae |
2.1.1.274 | additional information | an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout | Arabidopsis thaliana |
2.1.1.274 | additional information | an AtBSMT1 T-DNA knockout (AtBSMT1 KO, SALK_140496) is transformed by AtBSMT1 (Ask6) and PbBSMT (PsK5) and their susceptibility to clubroot is assessed. The infection rate is similar in the AtBSMT1 KO and AsK6 and comparable to that in wild-type. In contrast, PsK5 plants produce significantly larger galls than produced by the AtBSMT1 knockout | Plasmodiophora brassicae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.1.1.273 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
2.1.1.273 | extracellular | the enzyme is secreted | Plasmodiophora brassicae | - |
- |
2.1.1.274 | chloroplast | - |
Arabidopsis thaliana | 9507 | - |
2.1.1.274 | extracellular | the enzyme is secreted | Plasmodiophora brassicae | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.273 | S-adenosyl-L-methionine + benzoate | Arabidopsis thaliana | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + benzoate | Plasmodiophora brassicae | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + benzoate | Arabidopsis thaliana Col-0 | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + salicylate | Arabidopsis thaliana | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + salicylate | Plasmodiophora brassicae | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + salicylate | Arabidopsis thaliana Col-0 | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + benzoate | Arabidopsis thaliana | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + benzoate | Plasmodiophora brassicae | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + benzoate | Arabidopsis thaliana Col-0 | - |
methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + salicylate | Arabidopsis thaliana | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + salicylate | Plasmodiophora brassicae | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + salicylate | Arabidopsis thaliana Col-0 | - |
methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.273 | Arabidopsis thaliana | Q6XMI3 | - |
- |
2.1.1.273 | Arabidopsis thaliana Col-0 | Q6XMI3 | - |
- |
2.1.1.273 | Plasmodiophora brassicae | R4I7S9 | - |
- |
2.1.1.274 | Arabidopsis thaliana | Q6XMI3 | - |
- |
2.1.1.274 | Arabidopsis thaliana Col-0 | Q6XMI3 | - |
- |
2.1.1.274 | Plasmodiophora brassicae | R4I7S9 | - |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.273 | leaf | - |
Arabidopsis thaliana | - |
2.1.1.274 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.273 | S-adenosyl-L-methionine + benzoate | - |
Arabidopsis thaliana | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + benzoate | - |
Plasmodiophora brassicae | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + benzoate | - |
Arabidopsis thaliana Col-0 | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + salicylate | - |
Arabidopsis thaliana | methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + salicylate | - |
Plasmodiophora brassicae | methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.273 | S-adenosyl-L-methionine + salicylate | - |
Arabidopsis thaliana Col-0 | methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + benzoate | - |
Arabidopsis thaliana | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + benzoate | - |
Plasmodiophora brassicae | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + benzoate | - |
Arabidopsis thaliana Col-0 | methyl benzoate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + salicylate | - |
Arabidopsis thaliana | methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + salicylate | - |
Plasmodiophora brassicae | methyl salicylate + S-adenosyl-L-homocysteine | - |
? | |
2.1.1.274 | S-adenosyl-L-methionine + salicylate | - |
Arabidopsis thaliana Col-0 | methyl salicylate + S-adenosyl-L-homocysteine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.273 | More | PbBSMT has a salicylate- and an indole-3-acetic acid-binding domain | Plasmodiophora brassicae |
2.1.1.274 | More | PbBSMT has a salicylate- and an indole-3-acetic acid-binding domain | Plasmodiophora brassicae |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.273 | BSMT | - |
Arabidopsis thaliana |
2.1.1.273 | BSMT | - |
Plasmodiophora brassicae |
2.1.1.273 | More | see also EC 2.1.1.274 | Arabidopsis thaliana |
2.1.1.273 | More | see also EC 2.1.1.274 | Plasmodiophora brassicae |
2.1.1.274 | BSMT | - |
Arabidopsis thaliana |
2.1.1.274 | BSMT | - |
Plasmodiophora brassicae |
2.1.1.274 | More | see also EC 2.1.1.273 | Arabidopsis thaliana |
2.1.1.274 | More | see also EC 2.1.1.273 | Plasmodiophora brassicae |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.273 | S-adenosyl-L-methionine | - |
Arabidopsis thaliana | |
2.1.1.273 | S-adenosyl-L-methionine | - |
Plasmodiophora brassicae | |
2.1.1.274 | S-adenosyl-L-methionine | - |
Arabidopsis thaliana | |
2.1.1.274 | S-adenosyl-L-methionine | - |
Plasmodiophora brassicae |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.1.1.273 | malfunction | AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview | Arabidopsis thaliana |
2.1.1.273 | malfunction | basal salicylic acid (SA) levels in Arabidopsis thaliana plants that constitutively overexpress PbBSMT compared with those in Arabidopsis wild-type Col-0 are reduced approximately 80% versus only a 50% reduction in plants overexpressing AtBSMT1. PbBSMT-overexpressing plants are more susceptible to Plasmodiophora brassicae than wild-type plants, they also are partially compromised in nonhost resistance to Albugo candida. In contrast, AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Furthermore, transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum cv. Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview | Plasmodiophora brassicae |
2.1.1.273 | physiological function | mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus), encoding a protein with very limited homology to benzoic acid (BA)/SA-methyltransferase, designated PbBSMT. Enzyme PbBSMT is an effector, which is secreted by Plasmodiophora brassicae into its host plant to deplete pathogen-induced SA accumulation. Plasmodiophora brassicae uses PbBSMTto overcome SA-mediated defenses by converting SA into inactive methyl salicylate (MeSA). PbBSMT suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) | Plasmodiophora brassicae |
2.1.1.273 | physiological function | mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus). A pathogen salicylate methyltransferase, PbBSMT, suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host methyltransferase enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) | Arabidopsis thaliana |
2.1.1.274 | malfunction | AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview | Arabidopsis thaliana |
2.1.1.274 | malfunction | basal salicylic acid (SA) levels in Arabidopsis thaliana plants that constitutively overexpress PbBSMT compared with those in Arabidopsis wild-type Col-0 are reduced approximately 80% versus only a 50% reduction in plants overexpressing AtBSMT1. PbBSMT-overexpressing plants are more susceptible to Plasmodiophora brassicae than wild-type plants, they also are partially compromised in nonhost resistance to Albugo candida. In contrast, AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Furthermore, transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum cv. Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview | Plasmodiophora brassicae |
2.1.1.274 | physiological function | mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus), encoding a protein with very limited homology to benzoic acid (BA)/SA-methyltransferase, designated PbBSMT. Enzyme PbBSMT is an effector, which is secreted by Plasmodiophora brassicae into its host plant to deplete pathogen-induced SA accumulation. Plasmodiophora brassicae uses PbBSMTto overcome SA-mediated defenses by converting SA into inactive methyl salicylate (MeSA). PbBSMT suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) | Plasmodiophora brassicae |
2.1.1.274 | physiological function | mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus). A pathogen salicylate methyltransferase, PbBSMT, suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host methyltransferase enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI) | Arabidopsis thaliana |