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Literature summary extracted from

  • Sah, S.; Shah, R.; Govindan, A.; Varada, R.; Rex, K.; Varshney, U.
    Utilisation of 10-formyldihydrofolate as substrate by dihydrofolate reductase (DHFR) and 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) tranformylase/IMP cyclohydrolase (PurH) in Escherichia coli (2018), Microbiology, 164, 982-991 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.3
-
Escherichia coli
1.5.1.5
-
Escherichia coli
2.1.2.3
-
Escherichia coli
2.1.2.3 expressed in Escherichia coli Rosetta (DE3) pLysS cells Escherichia coli
3.5.4.10
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.003
-
10-formyl-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
1.5.1.3 0.0055
-
7,8-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
1.5.1.3 0.0074
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate Escherichia coli
1.5.1.3 0.008
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate Escherichia coli
2.1.2.3 0.0346
-
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide at pH 7.5 and 25°C Escherichia coli
2.1.2.3 0.111
-
10-formyltetrahydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
2.1.2.3 0.245
-
10-formyldihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
3.5.4.10 0.0346
-
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide pH 7.5, temperature not specified in the publication Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.3 22000
-
SDS-PAGE Escherichia coli
1.5.1.5 22000
-
SDS-PAGE Escherichia coli
2.1.2.3 22000
-
SDS-PAGE Escherichia coli
3.5.4.10 22000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.3 10-formyl-dihydrofolate + NADPH + H+ Escherichia coli the enzyme is involved in the folate recycling pathway 10-formyl-tetrahydrofolate + NADP+
-
?
1.5.1.3 10-formyl-dihydrofolate + NADPH + H+ Escherichia coli K12 the enzyme is involved in the folate recycling pathway 10-formyl-tetrahydrofolate + NADP+
-
?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Escherichia coli the enzyme is involved in the folate recycling pathway 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ Escherichia coli K12 the enzyme is involved in the folate recycling pathway 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Escherichia coli the enzyme is involved in the folate recycling pathway 5,10-methenyltetrahydrofolate + NADPH + H+
-
?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ Escherichia coli K12 the enzyme is involved in the folate recycling pathway 5,10-methenyltetrahydrofolate + NADPH + H+
-
?
2.1.2.3 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide Escherichia coli
-
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli P0ABQ4
-
-
1.5.1.3 Escherichia coli K12 P0ABQ4
-
-
1.5.1.5 Escherichia coli P24186
-
-
1.5.1.5 Escherichia coli K12 P24186
-
-
2.1.2.3 Escherichia coli
-
-
-
2.1.2.3 Escherichia coli P15639
-
-
2.1.2.3 Escherichia coli K12 P15639
-
-
3.5.4.10 Escherichia coli P15639
-
-
3.5.4.10 Escherichia coli K12 P15639
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
Escherichia coli
1.5.1.5
-
Escherichia coli
2.1.2.3
-
Escherichia coli
2.1.2.3 Ni-NTA column chromatography Escherichia coli
3.5.4.10
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 10-formyl-dihydrofolate + NADPH + H+
-
Escherichia coli 10-formyl-tetrahydrofolate + NADP+
-
?
1.5.1.3 10-formyl-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli 10-formyl-tetrahydrofolate + NADP+
-
?
1.5.1.3 10-formyl-dihydrofolate + NADPH + H+
-
Escherichia coli K12 10-formyl-tetrahydrofolate + NADP+
-
?
1.5.1.3 10-formyl-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli K12 10-formyl-tetrahydrofolate + NADP+
-
?
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.3 7,8-dihydrofolate + NADPH + H+
-
Escherichia coli K12 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.3 7,8-dihydrofolate + NADPH + H+ the enzyme is involved in the folate recycling pathway Escherichia coli K12 5,6,7,8-tetrahydrofolate + NADP+
-
?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
Escherichia coli 5,10-methenyltetrahydrofolate + NADPH + H+
-
?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ the enzyme is involved in the folate recycling pathway Escherichia coli 5,10-methenyltetrahydrofolate + NADPH + H+
-
?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+
-
Escherichia coli K12 5,10-methenyltetrahydrofolate + NADPH + H+
-
?
1.5.1.5 5,10-methylenetetrahydrofolate + NADP+ the enzyme is involved in the folate recycling pathway Escherichia coli K12 5,10-methenyltetrahydrofolate + NADPH + H+
-
?
2.1.2.3 10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
2.1.2.3 10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli K12 dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
2.1.2.3 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
2.1.2.3 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli K12 tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
?
3.5.4.10 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli IMP + H2O
-
?
3.5.4.10 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
-
Escherichia coli K12 IMP + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.5.1.3 ? x * 22000, SDS-PAGE Escherichia coli
1.5.1.5 ? x * 22000, SDS-PAGE Escherichia coli
2.1.2.3 ? x * 22000, SDS-PAGE Escherichia coli
3.5.4.10 ? x * 22000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 DHFR
-
Escherichia coli
1.5.1.3 folA
-
Escherichia coli
1.5.1.5 FolD
-
Escherichia coli
2.1.2.3 5-aminoimidazole-4-carboxamide ribonucleotide tranformylase
-
Escherichia coli
2.1.2.3 5-aminoimidazole-4-carboxamide ribonucleotide tranformylase/IMP cyclohydrolase
-
Escherichia coli
2.1.2.3 AICAR tranformylase
-
Escherichia coli
2.1.2.3 AICAR tranformylase/IMP cyclohydrolase
-
Escherichia coli
2.1.2.3 PurH
-
Escherichia coli
2.1.2.3 PurH bifunctional enzyme with tranformylase and IMP cyclohydrolase activities Escherichia coli
3.5.4.10 5-aminoimidazole-4-carboxamide ribonucleotide tranformylase/IMP cyclohydrolase
-
Escherichia coli
3.5.4.10 AICAR tranformylase/IMP cyclohydrolase
-
Escherichia coli
3.5.4.10 PurH
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.3 4.05
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate Escherichia coli
1.5.1.3 4.6
-
10-formyl-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
1.5.1.3 22.8
-
7,8-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
1.5.1.3 28.1
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate Escherichia coli
2.1.2.3 3.63
-
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide at pH 7.5 and 25°C Escherichia coli
2.1.2.3 4.97
-
10-formyltetrahydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
2.1.2.3 7.18
-
10-formyldihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
3.5.4.10 3.63
-
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide pH 7.5, temperature not specified in the publication Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 7.5
-
assay at Escherichia coli
1.5.1.5 7.5
-
assay at Escherichia coli
2.1.2.3 7.5
-
assay at Escherichia coli
3.5.4.10 7.5
-
assay at Escherichia coli

General Information

EC Number General Information Comment Organism
1.5.1.3 malfunction growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions Escherichia coli
1.5.1.3 metabolism the enzyme is involved in the folate recycling pathway Escherichia coli
1.5.1.5 metabolism the enzyme is involved in the folate recycling pathway Escherichia coli
2.1.2.3 malfunction growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions Escherichia coli
2.1.2.3 metabolism the enzyme is involved in the folate recycling pathway Escherichia coli
3.5.4.10 malfunction growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions Escherichia coli
3.5.4.10 metabolism the enzyme is involved in the folate recycling pathway Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.3 547.3
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate Escherichia coli
1.5.1.3 1533.3
-
10-formyl-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
1.5.1.3 3512.5
-
NADPH pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate Escherichia coli
1.5.1.3 4560
-
7,8-dihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
2.1.2.3 29.3
-
10-formyldihydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
2.1.2.3 44.77
-
10-formyltetrahydrofolate pH 7.5, temperature not specified in the publication Escherichia coli
2.1.2.3 4150
-
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide at pH 7.5 and 25°C Escherichia coli
3.5.4.10 104.9
-
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide pH 7.5, temperature not specified in the publication Escherichia coli