EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Escherichia coli |
1.5.1.5 | - |
Escherichia coli |
2.1.2.3 | - |
Escherichia coli |
2.1.2.3 | expressed in Escherichia coli Rosetta (DE3) pLysS cells | Escherichia coli |
3.5.4.10 | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.003 | - |
10-formyl-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0055 | - |
7,8-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
1.5.1.3 | 0.0074 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate | Escherichia coli | |
1.5.1.3 | 0.008 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate | Escherichia coli | |
2.1.2.3 | 0.0346 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | at pH 7.5 and 25°C | Escherichia coli | |
2.1.2.3 | 0.111 | - |
10-formyltetrahydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
2.1.2.3 | 0.245 | - |
10-formyldihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
3.5.4.10 | 0.0346 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.5, temperature not specified in the publication | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 22000 | - |
SDS-PAGE | Escherichia coli |
1.5.1.5 | 22000 | - |
SDS-PAGE | Escherichia coli |
2.1.2.3 | 22000 | - |
SDS-PAGE | Escherichia coli |
3.5.4.10 | 22000 | - |
SDS-PAGE | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 10-formyl-dihydrofolate + NADPH + H+ | Escherichia coli | the enzyme is involved in the folate recycling pathway | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 10-formyl-dihydrofolate + NADPH + H+ | Escherichia coli K12 | the enzyme is involved in the folate recycling pathway | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Escherichia coli | the enzyme is involved in the folate recycling pathway | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | Escherichia coli K12 | the enzyme is involved in the folate recycling pathway | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | Escherichia coli | the enzyme is involved in the folate recycling pathway | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | Escherichia coli K12 | the enzyme is involved in the folate recycling pathway | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
2.1.2.3 | 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | Escherichia coli | - |
tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Escherichia coli | P0ABQ4 | - |
- |
1.5.1.3 | Escherichia coli K12 | P0ABQ4 | - |
- |
1.5.1.5 | Escherichia coli | P24186 | - |
- |
1.5.1.5 | Escherichia coli K12 | P24186 | - |
- |
2.1.2.3 | Escherichia coli | - |
- |
- |
2.1.2.3 | Escherichia coli | P15639 | - |
- |
2.1.2.3 | Escherichia coli K12 | P15639 | - |
- |
3.5.4.10 | Escherichia coli | P15639 | - |
- |
3.5.4.10 | Escherichia coli K12 | P15639 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Escherichia coli |
1.5.1.5 | - |
Escherichia coli |
2.1.2.3 | - |
Escherichia coli |
2.1.2.3 | Ni-NTA column chromatography | Escherichia coli |
3.5.4.10 | - |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 10-formyl-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 10-formyl-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 10-formyl-dihydrofolate + NADPH + H+ | - |
Escherichia coli K12 | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 10-formyl-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli K12 | 10-formyl-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | - |
Escherichia coli K12 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.3 | 7,8-dihydrofolate + NADPH + H+ | the enzyme is involved in the folate recycling pathway | Escherichia coli K12 | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Escherichia coli | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | the enzyme is involved in the folate recycling pathway | Escherichia coli | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | - |
Escherichia coli K12 | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
1.5.1.5 | 5,10-methylenetetrahydrofolate + NADP+ | the enzyme is involved in the folate recycling pathway | Escherichia coli K12 | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? | |
2.1.2.3 | 10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
2.1.2.3 | 10-formyldihydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli K12 | dihydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
2.1.2.3 | 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli | tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
2.1.2.3 | 10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli K12 | tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
? | |
3.5.4.10 | 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli | IMP + H2O | - |
? | |
3.5.4.10 | 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | - |
Escherichia coli K12 | IMP + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.3 | ? | x * 22000, SDS-PAGE | Escherichia coli |
1.5.1.5 | ? | x * 22000, SDS-PAGE | Escherichia coli |
2.1.2.3 | ? | x * 22000, SDS-PAGE | Escherichia coli |
3.5.4.10 | ? | x * 22000, SDS-PAGE | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.3 | DHFR | - |
Escherichia coli |
1.5.1.3 | folA | - |
Escherichia coli |
1.5.1.5 | FolD | - |
Escherichia coli |
2.1.2.3 | 5-aminoimidazole-4-carboxamide ribonucleotide tranformylase | - |
Escherichia coli |
2.1.2.3 | 5-aminoimidazole-4-carboxamide ribonucleotide tranformylase/IMP cyclohydrolase | - |
Escherichia coli |
2.1.2.3 | AICAR tranformylase | - |
Escherichia coli |
2.1.2.3 | AICAR tranformylase/IMP cyclohydrolase | - |
Escherichia coli |
2.1.2.3 | PurH | - |
Escherichia coli |
2.1.2.3 | PurH | bifunctional enzyme with tranformylase and IMP cyclohydrolase activities | Escherichia coli |
3.5.4.10 | 5-aminoimidazole-4-carboxamide ribonucleotide tranformylase/IMP cyclohydrolase | - |
Escherichia coli |
3.5.4.10 | AICAR tranformylase/IMP cyclohydrolase | - |
Escherichia coli |
3.5.4.10 | PurH | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 4.05 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate | Escherichia coli | |
1.5.1.3 | 4.6 | - |
10-formyl-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
1.5.1.3 | 22.8 | - |
7,8-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
1.5.1.3 | 28.1 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate | Escherichia coli | |
2.1.2.3 | 3.63 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | at pH 7.5 and 25°C | Escherichia coli | |
2.1.2.3 | 4.97 | - |
10-formyltetrahydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
2.1.2.3 | 7.18 | - |
10-formyldihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
3.5.4.10 | 3.63 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.5, temperature not specified in the publication | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 7.5 | - |
assay at | Escherichia coli |
1.5.1.5 | 7.5 | - |
assay at | Escherichia coli |
2.1.2.3 | 7.5 | - |
assay at | Escherichia coli |
3.5.4.10 | 7.5 | - |
assay at | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.1.3 | malfunction | growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions | Escherichia coli |
1.5.1.3 | metabolism | the enzyme is involved in the folate recycling pathway | Escherichia coli |
1.5.1.5 | metabolism | the enzyme is involved in the folate recycling pathway | Escherichia coli |
2.1.2.3 | malfunction | growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions | Escherichia coli |
2.1.2.3 | metabolism | the enzyme is involved in the folate recycling pathway | Escherichia coli |
3.5.4.10 | malfunction | growth deficiency phenotypes (in un-supplemented M9 minimal medium containing thymidine) are direct consequences of the gene deletions | Escherichia coli |
3.5.4.10 | metabolism | the enzyme is involved in the folate recycling pathway | Escherichia coli |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 547.3 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate | Escherichia coli | |
1.5.1.3 | 1533.3 | - |
10-formyl-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
1.5.1.3 | 3512.5 | - |
NADPH | pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate | Escherichia coli | |
1.5.1.3 | 4560 | - |
7,8-dihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
2.1.2.3 | 29.3 | - |
10-formyldihydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
2.1.2.3 | 44.77 | - |
10-formyltetrahydrofolate | pH 7.5, temperature not specified in the publication | Escherichia coli | |
2.1.2.3 | 4150 | - |
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | at pH 7.5 and 25°C | Escherichia coli | |
3.5.4.10 | 104.9 | - |
5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide | pH 7.5, temperature not specified in the publication | Escherichia coli |