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Literature summary extracted from

  • Miao, Y.; Tenor, J.L.; Toffaletti, D.L.; Maskarinec, S.A.; Liu, J.; Lee, R.E.; Perfect, J.R.; Brennan, R.G.
    Structural and in vivo studies on trehalose-6-phosphate synthase from pathogenic fungi provide insights into its catalytic mechanism, biological necessity, and potential for novel antifungal drug design (2017), mBio, 8, e00643-17 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.15 expressed in Escherichia coli BL21(DE3)pLysS cells Candida albicans
2.4.1.15 expressed in Escherichia coli BL21(DE3)pLysS cells Aspergillus fumigatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.1.15 enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.1 M sodium malonate, pH 8.0, 0.1 M Tris, pH 8.0, and 30% (w/v) polyethylene glycol 1000 Aspergillus fumigatus
2.4.1.15 enzyme bound to either UDPG or UDP-alpha-D-glucose and D-glucose 6-phosphate or inhibitor validoxylamine A and UDP, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, and 40% (w/v) polyethylene glycol 400 Candida albicans

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.15 D379A inactive Candida albicans
2.4.1.15 E387A inactive Candida albicans
2.4.1.15 K285A inactive Candida albicans
2.4.1.15 Y89F inactive Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.15 validoxylamine A
-
 Aspergillus fumigatus
2.4.1.15 validoxylamine A
-
 Candida albicans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.15 Mg2+ 5 mM used in assay conditions Candida albicans
2.4.1.15 Mg2+ 5 mM used in assay conditions Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Candida albicans
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Aspergillus fumigatus
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate Aspergillus fumigatus Af293
-
 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.15 Aspergillus fumigatus Q4WLM9
-
-
2.4.1.15 Aspergillus fumigatus Af293 Q4WLM9
-
-
2.4.1.15 Candida albicans Q92410
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.15 Ni-NTA column chromatography and Superdex S200 gel filtration Candida albicans
2.4.1.15 Ni-NTA column chromatography and Superdex S200 gel filtration Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Candida albicans UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Aspergillus fumigatus UDP + alpha,alpha-trehalose 6-phosphate
-
 ?
2.4.1.15 UDP-alpha-D-glucose + D-glucose 6-phosphate
-
 Aspergillus fumigatus Af293 UDP + alpha,alpha-trehalose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.15 TPS1
-
 Candida albicans
2.4.1.15 TPS1
-
 Aspergillus fumigatus
2.4.1.15 trehalose-6-phosphate synthase
-
 Candida albicans
2.4.1.15 trehalose-6-phosphate synthase
-
 Aspergillus fumigatus