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Literature summary extracted from

  • Singh, A.; Mohammad Arif, S.; Biak Sang, P.; Varshney, U.; Vijayan, M.
    Structural insights into the specificity and catalytic mechanism of mycobacterial nucleotide pool sanitizing enzyme MutT2 (2018), J. Struct. Biol., 204, 449-456 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.55
-
 Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.6.1.55 apoenzyme and its complexes with products (dCMP, CMP and 5-methyl-dCMP) or substrates (dCTP, CTP and 5-methyl-dCTP), using either 0.1 M imidazole pH 6.5, 1.0 M sodium acetate trihydrate (product complexes), 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 30% (w/v) PEG 4000 (substrate complexes), 0.1 M MES monohydrate pH 6.0, 20% (v/v) Jeffamine M-600 pH 7.0 or 0.2 M ammonium sulfate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30% (w/v) PEG 8000 (apoenzyme) Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.55 Mg2+ required Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.55 Mycolicibacterium smegmatis A0R2K6
-
-
3.6.1.55 Mycolicibacterium smegmatis mc(2)155 A0R2K6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.55
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.55 5-methyl-dCTP + H2O
-
 Mycolicibacterium smegmatis 5-methyl-dCMP + diphosphate
-
 ?
3.6.1.55 5-methyl-dCTP + H2O
-
 Mycolicibacterium smegmatis mc(2)155 5-methyl-dCMP + diphosphate
-
 ?
3.6.1.55 8-oxo-dGTP + H2O
-
 Mycolicibacterium smegmatis 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.55 8-oxo-dGTP + H2O
-
 Mycolicibacterium smegmatis mc(2)155 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.55 CTP + H2O
-
 Mycolicibacterium smegmatis CMP + diphosphate
-
 ?
3.6.1.55 CTP + H2O
-
 Mycolicibacterium smegmatis mc(2)155 CMP + diphosphate
-
 ?
3.6.1.55 dCTP + H2O
-
 Mycolicibacterium smegmatis dCMP + diphosphate
-
 ?
3.6.1.55 dCTP + H2O
-
 Mycolicibacterium smegmatis mc(2)155 dCMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.55 MutT2
-
 Mycolicibacterium smegmatis