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Literature summary extracted from

  • Lee, D.; Park, H.L.; Lee, S.W.; Bhoo, S.H.; Cho, M.H.
    Biotechnological production of dimethoxyflavonoids using a fusion flavonoid O-methyltransferase possessing both 3- and 7-O-methyltransferase activities (2017), J. Nat. Prod., 80, 1467-1474 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.82 expressed as fusion protein with Solanum lycopersiucm 3'-O-methyltransferase OMT3 in Escherichia coli Oryza sativa
2.1.1.82 recombinant expression of wild-type and chimeric mutant enzymes in Escherichia coli Oryza sativa
2.1.1.267 recombinant expression of wild-type anchimeric mutant enzymes in Escherichia coli Solanum lycopersicum

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.82 additional information generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene Oryza sativa
2.1.1.232 additional information construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively Solanum lycopersicum
2.1.1.267 additional information construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively Solanum lycopersicum
2.1.1.267 additional information generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.267 S-adenosyl-L-methionine + a 3'-hydroxyflavonoid Solanum lycopersicum
-
S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.82 Oryza sativa
-
-
-
2.1.1.232 Solanum lycopersicum
-
-
-
2.1.1.267 Solanum lycopersicum
-
-
-
2.1.1.267 Solanum lycopersicum K4D2J5
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.82 2 S-adenosyl-L-methionine + eriodictyol
-
Oryza sativa 2 S-adenosyl-L-homocysteine + 3',7-di-O-methyleriodictyol
-
?
2.1.1.82 2 S-adenosyl-L-methionine + luteolin
-
Oryza sativa 2 S-adenosyl-L-homocysteine + velutin
-
?
2.1.1.82 2 S-adenosyl-L-methionine + quercetin
-
Oryza sativa 2 S-adenosyl-L-homocysteine + rhamnazin
-
?
2.1.1.82 2 S-adenosyl-L-methionine + taxifolin
-
Oryza sativa 2 S-adenosyl-L-homocysteine + 3',7-di-O-methyltaxifolin
-
?
2.1.1.267 S-adenosyl-L-methionine + a 3'-hydroxyflavonoid
-
Solanum lycopersicum S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid
-
?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.82 7-O-methyltransferase
-
Oryza sativa
2.1.1.82 7-OMT
-
Oryza sativa
2.1.1.82 flavonoid O-methyltransferase
-
Oryza sativa
2.1.1.82 NOMT
-
Oryza sativa
2.1.1.82 OsOMT7
-
Oryza sativa
2.1.1.267 3'-OMT
-
Solanum lycopersicum
2.1.1.267 flavonoid O-methyltransferase
-
Solanum lycopersicum
2.1.1.267 SlOMT3
-
Solanum lycopersicum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.82 S-adenosyl-L-methionine
-
Oryza sativa
2.1.1.267 S-adenosyl-L-methionine
-
Solanum lycopersicum