EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.82 | expressed as fusion protein with Solanum lycopersiucm 3'-O-methyltransferase OMT3 in Escherichia coli | Oryza sativa |
2.1.1.82 | recombinant expression of wild-type and chimeric mutant enzymes in Escherichia coli | Oryza sativa |
2.1.1.267 | recombinant expression of wild-type anchimeric mutant enzymes in Escherichia coli | Solanum lycopersicum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.82 | additional information | generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene | Oryza sativa |
2.1.1.232 | additional information | construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively | Solanum lycopersicum |
2.1.1.267 | additional information | construction of a multifunctional flavonoid O-methyltransferase fusing tomato 3'-O-methyltransferase OMT3 and Oryza sativa naringenin 7-O-methyltransferase NOMT. The OMT3/NOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual OMT3 and NOMT. The OMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using OMT3/NOMT-transformed Escherichia coli generates corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-Omethyltaxifolin, respectively | Solanum lycopersicum |
2.1.1.267 | additional information | generation of a multifunctional FOMT fusing a 3'-OMT (SlOMT3, EC 2.1.1.267) and a 7-OMT (OsNOMT, EC 2.1.1.82). The SlOMT3/OsNOMT fusion enzyme possesses both 3'- and 7-OMT activities to diverse flavonoid substrates, which are comparable to those of individual SlOMT3 and OsNOMT. The SlOMT3/OsNOMT enzyme also shows 3'- and 7-OMT activity for 7- or 3'-O-methylflavonoids, respectively, suggesting that the fusion enzyme can sequentially methylate flavonoids into di-O-methylflavonoids. The biotransformation of the flavonoids quercetin, luteolin, eriodictyol, and taxifolin using SlOMT3/OsNOMT-transformed Escherichia coli generated corresponding di-O-methylflavonoids, rhamnazin, velutin, 3',7-di-O-methyleriodictyol, and 3',7-di-O-methyltaxifolin, respectively. These results indicate that dimethoxyflavonoids may be efficiently produced from non-methylated flavonoid precursors through a one-step biotransformation using the engineered Escherichia coli harboring the SlOMT3/OsNOMT fusion gene | Solanum lycopersicum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.267 | S-adenosyl-L-methionine + a 3'-hydroxyflavonoid | Solanum lycopersicum | - |
S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.82 | Oryza sativa | - |
- |
- |
2.1.1.232 | Solanum lycopersicum | - |
- |
- |
2.1.1.267 | Solanum lycopersicum | - |
- |
- |
2.1.1.267 | Solanum lycopersicum | K4D2J5 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.82 | 2 S-adenosyl-L-methionine + eriodictyol | - |
Oryza sativa | 2 S-adenosyl-L-homocysteine + 3',7-di-O-methyleriodictyol | - |
? | |
2.1.1.82 | 2 S-adenosyl-L-methionine + luteolin | - |
Oryza sativa | 2 S-adenosyl-L-homocysteine + velutin | - |
? | |
2.1.1.82 | 2 S-adenosyl-L-methionine + quercetin | - |
Oryza sativa | 2 S-adenosyl-L-homocysteine + rhamnazin | - |
? | |
2.1.1.82 | 2 S-adenosyl-L-methionine + taxifolin | - |
Oryza sativa | 2 S-adenosyl-L-homocysteine + 3',7-di-O-methyltaxifolin | - |
? | |
2.1.1.267 | S-adenosyl-L-methionine + a 3'-hydroxyflavonoid | - |
Solanum lycopersicum | S-adenosyl-L-homocysteine + a 3'-methoxyflavonoid | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.82 | 7-O-methyltransferase | - |
Oryza sativa |
2.1.1.82 | 7-OMT | - |
Oryza sativa |
2.1.1.82 | flavonoid O-methyltransferase | - |
Oryza sativa |
2.1.1.82 | NOMT | - |
Oryza sativa |
2.1.1.82 | OsOMT7 | - |
Oryza sativa |
2.1.1.267 | 3'-OMT | - |
Solanum lycopersicum |
2.1.1.267 | flavonoid O-methyltransferase | - |
Solanum lycopersicum |
2.1.1.267 | SlOMT3 | - |
Solanum lycopersicum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.82 | S-adenosyl-L-methionine | - |
Oryza sativa | |
2.1.1.267 | S-adenosyl-L-methionine | - |
Solanum lycopersicum |