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Literature summary extracted from

  • Lee, H.S.; Kim, T.S.; Parajuli, P.; Pandey, R.P.; Sohng, J.K.
    Sustainable production of dihydroxybenzene glucosides using immobilized amylosucrase from Deinococcus geothermalis (2018), J. Microbiol. Biotechnol., 28, 1447-1456 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.4 synthesis immobilization enhances the efficiency of the glycosylation reaction and is therefore considered effective for industrial application in sustainable production of dihydroxybenzene glucosides Deinococcus geothermalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.4 codon-optimized and expressed in Escherichia coli BL21 (DE3) Deinococcus geothermalis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.4 Deinococcus geothermalis Q1J0W0
-
-
2.4.1.4 Deinococcus geothermalis DSM 11300 Q1J0W0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.4
-
 Deinococcus geothermalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.4 sucrose + catechol
-
 Deinococcus geothermalis D-fructose + catechol glucoside
-
 ?
2.4.1.4 sucrose + catechol
-
 Deinococcus geothermalis DSM 11300 D-fructose + catechol glucoside
-
 ?
2.4.1.4 sucrose + hydroquinone
-
 Deinococcus geothermalis D-fructose + hydroquinone alpha-glucoside
-
 ?
2.4.1.4 sucrose + hydroquinone
-
 Deinococcus geothermalis DSM 11300 D-fructose + hydroquinone alpha-glucoside
-
 ?
2.4.1.4 sucrose + resorcinol
-
 Deinococcus geothermalis D-fructose + resorcinol glucoside
-
 ?
2.4.1.4 sucrose + resorcinol
-
 Deinococcus geothermalis DSM 11300 D-fructose + resorcinol glucoside
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.4 35
-
 substrate: resorcinol or catechol Deinococcus geothermalis
2.4.1.4 40
-
 substrate: hydroquinone Deinococcus geothermalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.4 7
-
 highest yield with hydroquinone (98% conversion) or resorcinol (43.6% conversion) as substrate Deinococcus geothermalis
2.4.1.4 8
-
 highest yield with catechol (60.6% conversion) as substrate Deinococcus geothermalis