Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Zhong, W.; Pasunooti, K.K.; Balamkundu, S.; Wong, Y.H.; Nah, Q.; Gadi, V.; Gnanakalai, S.; Chionh, Y.H.; McBee, M.E.; Gopal, P.; Lim, S.H.; Olivier, N.; Buurman, E.T.; Dick, T.; Liu, C.F.; Lescar, J.; Dedon, P.C.
    Thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism (2019), J. Med. Chem., 62, 7788-7805 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228 gene trmD, sequence comparisons Staphylococcus aureus
2.1.1.228 gene trmD, sequence comparisons Pseudomonas aeruginosa
2.1.1.228 gene trmD, sequence comparisons Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.228 analysis of the crystal structure of apo-enzyme, of enzyme with bound ligand S-adenosyl-L-homocysteine (PDB IDs 5ZHI and 5ZHJ, respectively), and of enzyme with bound inhibitors N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide , and N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, PDB IDs 6JOF, 5ZHK, and 5ZHL, respectively, at resolutions of 1.75-2.76 A. Sitting drop vapor diffusion method, mixing of 0.002 ml of protein solution with 0.001 ml of precipitant solution containing 100 mM Bis-Tris propane, pH 6.5, 20% w/v PEG3350, and 0.1 M ammonium acetate, soaking of crystals with 1-5 mM inhibitor or 5 mM SAH in their respective precipitating solution supplemented with 20% v/v glycerol, X-ray diffraction structure determination and analysis Mycobacterium tuberculosis
2.1.1.228 analysis of the crystal structure of enzyme with bound inhibitors N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, N-(4-((diethylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide, and N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, PDB IDs 6JOE, 5ZHM, and 5ZHN, respectively. Hanging drop vapor diffusion method, mixing of equal volumes of 20 mg/m protein and precipitant solution containing 0.1 M Tris-HCl, pH 8.6-8.8, 20% v/v MPD, 20% w/v PEG 1000, and 5% w/v PEG200, and incubation at 20°C, soaking of crystals with 1-5 mM inhibitor in the precipitating solution supplemented with 20% v/v glycerol at 20°C for at least 4 h, X-ray diffraction structure determination and analysis Pseudomonas aeruginosa

General Stability

EC Number General Stability Organism
2.1.1.228 stabilization of the enzyme by binding of small molecules, overview Staphylococcus aureus
2.1.1.228 stabilization of the enzyme by binding of small molecules, overview Pseudomonas aeruginosa
2.1.1.228 stabilization of the enzyme by binding of small molecules, overview Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.228 4-((4-azidobenzyl)oxy)-N-(4-((octylamino)methyl)benzyl)thieno-[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 4-(hexyloxy)-N-(4-((octylamino)methyl)benzyl)thieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 4-methoxy-N-(4-(morpholinomethyl)benzyl)thieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 4-oxo-N-(4-((pentylamino)methyl)benzyl)-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 4-oxo-N-(4-(piperidin-1-ylmethyl)benzyl)-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 additional information synthesis of thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism, overview. The tyrosine-flipping mechanism is uniquely found in Pseudomonas aeruginosa TrmD and renders the enzyme inaccessible to the cofactor S-adenosyl-L-methionine (SAM) and probably to the substrate tRNA Mycobacterium tuberculosis
2.1.1.228 additional information synthesis of thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism, nanomolar potency against TrmD in vitro, overview. This tyrosine-flipping mechanism is uniquely found in Pseudomonas aeruginosa TrmD and renders the enzyme inaccessible to the cofactor S-adenosyl-L-methionine (SAM) and probably to the substrate tRNA. Biochemical structure-activity relationships (SAR) for TrmD inhibitors, the thienopyrimidinone substituent flexibility is critical for potent TrmD inhibition. Analysis of hemolytic activity of the compounds. Effect of side chain length of 15 analogues Pseudomonas aeruginosa
2.1.1.228 additional information synthesis of thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism, overview. The tyrosine-flipping mechanism is uniquely found in Pseudomonas aeruginosa TrmD and renders the enzyme inaccessible to the cofactor S-adenosyl-L-methionine (SAM) and probably to the substrate tRNA Staphylococcus aureus
2.1.1.228 N-(4-((((3s,5s,7s)-adamantan-1-yl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((2-(2-(2-aminoethoxy)ethoxy)ethyl)amino)methyl)-benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((2-(2-ethoxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((2-(2-hydroxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((4-aminobutyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide dihydrochloride
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((5-(1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)pentyl)amino) methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((5-aminopentyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(((7-aminohexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzyl(hexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzylamino)methyl)benzyl)-4-(hexyloxy)thieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzylamino)methyl)benzyl)-4-methoxythieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((benzylamino)methyl)benzyl)-4-propoxythieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((butylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide enzyme-bound crystal structure, overview Mycobacterium tuberculosis
2.1.1.228 N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((cyclohexylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((decylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((diethylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide enzyme-bound crystal structure, overview Pseudomonas aeruginosa
2.1.1.228 N-(4-((dodecylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((ethyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((octylamino)methyl)benzyl)-4-moxythieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide enzyme-bound crystal structure, overview Mycobacterium tuberculosis
2.1.1.228 N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide enzyme-bound crystal structure, overview Pseudomonas aeruginosa
2.1.1.228 N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Staphylococcus aureus
2.1.1.228 N-(4-((octylamino)methyl)benzyl)-4-propoxythieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(morpholinomethyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-(4-(morpholinomethyl)benzyl)-4-propoxythieno[2,3-d]-pyrimidine-5-carboxamide
-
 Pseudomonas aeruginosa
2.1.1.228 N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide the binding of AZ51 does not induce the side chain flip of Tyr111 in MtbTrmD, while the corresponding residue Tyr120 in PaTrmD turned 180° to form stacking interactions with the phenyl ring of the inhibitor, enzyme-bound crystal structure, overview Mycobacterium tuberculosis
2.1.1.228 N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide i.e. AZ51, inhibitor binding induces conformational changes of the wall loop, whereupon the side chain of aromatic ring of Tyr120 flips about 180° and forms stacking interactions with both the phenyl and piperidine rings of AZ51. This feature appears unique to AZ51 and P Pseudomonas aeruginosa
2.1.1.228 N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
-
 Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.228 additional information
-
 additional information binding kinetics of TrmD ligands Staphylococcus aureus
2.1.1.228 additional information
-
 additional information binding kinetics of TrmD ligands Pseudomonas aeruginosa
2.1.1.228 additional information
-
 additional information binding kinetics of TrmD ligands Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.228 Mg2+ required Pseudomonas aeruginosa
2.1.1.228 Mg2+ required Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Staphylococcus aureus
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Pseudomonas aeruginosa
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Mycobacterium tuberculosis
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Mycobacterium tuberculosis
-
-
-
2.1.1.228 Pseudomonas aeruginosa
-
-
-
2.1.1.228 Staphylococcus aureus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Staphylococcus aureus S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Pseudomonas aeruginosa S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Mycobacterium tuberculosis S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 bacterial tRNA (guanine37-N1)-methyltransferase
-
 Staphylococcus aureus
2.1.1.228 bacterial tRNA (guanine37-N1)-methyltransferase
-
 Pseudomonas aeruginosa
2.1.1.228 bacterial tRNA (guanine37-N1)-methyltransferase
-
 Mycobacterium tuberculosis
2.1.1.228 MtbTrmD
-
 Mycobacterium tuberculosis
2.1.1.228 PaTrmD
-
 Pseudomonas aeruginosa
2.1.1.228 SaTrmD
-
 Staphylococcus aureus
2.1.1.228 TrmD
-
 Staphylococcus aureus
2.1.1.228 TrmD
-
 Pseudomonas aeruginosa
2.1.1.228 TrmD
-
 Mycobacterium tuberculosis
2.1.1.228 tRNA (guanine37-N1)-methyltransferase
-
 Staphylococcus aureus
2.1.1.228 tRNA (guanine37-N1)-methyltransferase
-
 Pseudomonas aeruginosa
2.1.1.228 tRNA (guanine37-N1)-methyltransferase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.228 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.228 8
-
 assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.228 S-adenosyl-L-methionine
-
 Staphylococcus aureus
2.1.1.228 S-adenosyl-L-methionine
-
 Pseudomonas aeruginosa
2.1.1.228 S-adenosyl-L-methionine
-
 Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1.1.228 0.000024
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.000025
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((decylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00008
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((4-aminobutyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide dihydrochloride
2.1.1.228 0.000085
-
 pH 8.0, 37°C Pseudomonas aeruginosa 4-oxo-N-(4-((pentylamino)methyl)benzyl)-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00011
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((2-(2-hydroxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00011
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((5-aminopentyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00013
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((benzylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00014
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((7-aminohexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00015
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((butylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00018
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00021
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((2-(2-ethoxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00037
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((ethyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00038
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((dodecylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00045
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((2-(2-(2-aminoethoxy)ethoxy)ethyl)amino)methyl)-benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00049
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.0007
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]-pyrimidine-5-carboxamide
2.1.1.228 0.00072
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((((3s,5s,7s)-adamantan-1-yl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00073
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((diethylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.00079
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((cyclohexylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.0011
-
 pH 8.0, 37°C Pseudomonas aeruginosa 4-oxo-N-(4-(piperidin-1-ylmethyl)benzyl)-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.0013
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(((5-(1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)pentyl)amino) methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.0018
-
 pH 8.0, 37°C Pseudomonas aeruginosa 4-((4-azidobenzyl)oxy)-N-(4-((octylamino)methyl)benzyl)thieno-[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.0022
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((benzyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.0022
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((benzyl(hexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.003
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-(morpholinomethyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide
2.1.1.228 0.012
-
 pH 8.0, 37°C Pseudomonas aeruginosa N-(4-((benzyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide

General Information

EC Number General Information Comment Organism
2.1.1.228 physiological function bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) catalyzes methyl transfer from S-adenosyl-L-methionine (SAM) to the guanine N1 at nucleotide position 37 in a subset of bacterial tRNA isoacceptors and has proven to be an essential enzyme in most bacterial species Staphylococcus aureus
2.1.1.228 physiological function bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) catalyzes methyl transfer from S-adenosyl-L-methionine (SAM) to the guanine N1 at nucleotide position 37 in a subset of bacterial tRNA isoacceptors and has proven to be an essential enzyme in most bacterial species Pseudomonas aeruginosa
2.1.1.228 physiological function bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) catalyzes methyl transfer from S-adenosyl-L-methionine (SAM) to the guanine N1 at nucleotide position 37 in a subset of bacterial tRNA isoacceptors and has proven to be an essential enzyme in most bacterial species Mycobacterium tuberculosis