Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kimura, Y.; Kajimoto, S.; Yamamoto, Y.; Tanaka, N.
    Enzymatic characteristics of Nudix hydrolase 2 (Nud2), an 8-oxo-dGTP hydrolase from Myxococcus xanthus (2020), J. Gen. Appl. Microbiol., 66, 46-50 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.56 A84G the mutant shows about 70% of wild type activity Myxococcus xanthus
3.6.1.56 A84G/G90E the mutant shows about 110% of wild type activity Myxococcus xanthus
3.6.1.56 G90E the mutant shows about 130% of wild type activity Myxococcus xanthus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.56 0.025
-
 2-hydroxy-dATP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.027
-
 8-oxo-dGTP in the presence of 0.25 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.027
-
 8-oxo-dGDP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.03
-
 8-oxo-dATP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.046
-
 dGTP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.134
-
 dGDP in the presence of 5 mM Mg2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.298
-
 dATP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.319
-
 GDP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.385
-
 Ap4A in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.447
-
 dADP in the presence of 5 mM Mg2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.455
-
 ATP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.598
-
 GTP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 1.173
-
 ADP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.56 Co2+ Co2+ (5 mM) stimulates most enzyme hydrolase activities, with the exception of 8-oxo-dATP and Ap4A Myxococcus xanthus
3.6.1.56 Mg2+ 5 mm Mg2+ is not effective in stimulating enzyme-mediated 8-oxo-dATP, ATP, and Ap4A hydrolysis, however, 5 mM Mg2+ is the most effective in hydrolysing dADP and dGDP Myxococcus xanthus
3.6.1.56 Mn2+ most effective cofactor. The optimal concentrations of Mn2+ for enzyme activity toward 8-oxo-dGTP and dGTP are 0.25 mM and 0.75 mM, respectively Myxococcus xanthus

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.56 Myxococcus xanthus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.56 2-hydroxy-dATP + H2O
-
 Myxococcus xanthus 2-hydroxy-dAMP + diphosphate
-
 ?
3.6.1.56 8-oxo-dATP + H2O
-
 Myxococcus xanthus 8-oxo-dAMP + diphosphate
-
 ?
3.6.1.56 8-oxo-dGDP + H2O
-
 Myxococcus xanthus 8-oxo-dGMP + phosphate
-
 ?
3.6.1.56 8-oxo-dGTP + H2O highest specific activity toward 8-oxo-dGTP Myxococcus xanthus 8-oxo-dGMP + diphosphate
-
 ?
3.6.1.56 ADP + H2O
-
 Myxococcus xanthus AMP + phosphate
-
 ?
3.6.1.56 Ap4A + H2O
-
 Myxococcus xanthus AMP + ?
-
 ?
3.6.1.56 ATP + H2O
-
 Myxococcus xanthus AMP + diphosphate
-
 ?
3.6.1.56 dADP + H2O
-
 Myxococcus xanthus dAMP + phosphate
-
 ?
3.6.1.56 dATP + H2O
-
 Myxococcus xanthus dAMP + diphosphate
-
 ?
3.6.1.56 dGDP + H2O
-
 Myxococcus xanthus dGMP + phosphate
-
 ?
3.6.1.56 dGTP + H2O
-
 Myxococcus xanthus dGMP + diphosphate
-
 ?
3.6.1.56 GDP + H2O
-
 Myxococcus xanthus GMP + phosphate
-
 ?
3.6.1.56 GTP + H2O
-
 Myxococcus xanthus GMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.56 8-oxo-dGTP hydrolase
-
 Myxococcus xanthus
3.6.1.56 Nud2
-
 Myxococcus xanthus
3.6.1.56 Nudix hydrolase 2
-
 Myxococcus xanthus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.56 0.097
-
 2-hydroxy-dATP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.102
-
 ADP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.103
-
 8-oxo-dATP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.103
-
 Ap4A in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.128
-
 dGDP in the presence of 5 mM Mg2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.143
-
 dADP in the presence of 5 mM Mg2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.15
-
 dATP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.158
-
 ATP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.187
-
 GDP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.273
-
 8-oxo-dGDP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.308
-
 dGTP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.338
-
 GTP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 1.13
-
 8-oxo-dGTP in the presence of 0.25 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.6.1.56 0.09
-
 ADP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.27
-
 Ap4A in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.6
-
 GTP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 0.95
-
 dGDP in the presence of 5 mM Mg2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 3.2
-
 dADP in the presence of 5 mM Mg2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 3.5
-
 8-oxo-dATP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 3.5
-
 ATP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 3.8
-
 2-hydroxy-dATP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 5
-
 dATP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 5.8
-
 GDP in the presence of 5 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 6.7
-
 dGTP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 10.2
-
 8-oxo-dGDP in the presence of 1 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus
3.6.1.56 41.7
-
 8-oxo-dGTP in the presence of 0.25 mM Mn2+, at pH 8.0 and 37°C Myxococcus xanthus