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Literature summary extracted from

  • Zhou, M.; Xue, L.; Chen, Y.; Li, H.; He, Q.; Wang, B.; Meng, F.; Wang, M.; Guan, M.X.
    A hypertension-associated mitochondrial DNA mutation introduces an m1G37 modification into tRNAMet, altering its structure and function (2018), J. Biol. Chem., 293, 1425-1438 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.228 additional information the m.4435A->G mutation introduces an m1G37 modification of tRNAMet, altering its structure and function. Primer extension and methylation activity assays indeed confirm that the m.4435A3G mutation creates a tRNA methyltransferase 5 (TRMT5)-catalyzed m1G37 modification of tRNAMet Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii NBRC 100440
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii DSM 2661
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii ATCC 43067
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii JAL-1
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii JCM 10045
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Methanocaldococcus jannaschii Q58293 i.e. Methanococcus jannaschii
-
2.1.1.228 Methanocaldococcus jannaschii ATCC 43067 Q58293 i.e. Methanococcus jannaschii
-
2.1.1.228 Methanocaldococcus jannaschii DSM 2661 Q58293 i.e. Methanococcus jannaschii
-
2.1.1.228 Methanocaldococcus jannaschii JAL-1 Q58293 i.e. Methanococcus jannaschii
-
2.1.1.228 Methanocaldococcus jannaschii JCM 10045 Q58293 i.e. Methanococcus jannaschii
-
2.1.1.228 Methanocaldococcus jannaschii NBRC 100440 Q58293 i.e. Methanococcus jannaschii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 additional information the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet Methanocaldococcus jannaschii ?
-
-
2.1.1.228 additional information the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet Methanocaldococcus jannaschii NBRC 100440 ?
-
-
2.1.1.228 additional information the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet Methanocaldococcus jannaschii DSM 2661 ?
-
-
2.1.1.228 additional information the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet Methanocaldococcus jannaschii ATCC 43067 ?
-
-
2.1.1.228 additional information the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet Methanocaldococcus jannaschii JAL-1 ?
-
-
2.1.1.228 additional information the mutant tRNAMet transcripts (G37) are modified with m1G37 modification by the Mj-Trm5 but as less efficiently as cytoplasmic tRNALeu(CAG) transcripts. In contrast, the modification is not detected in the human wild-type tRNAMet transcripts (A37) in the presence of Mj-Trm5. The human cytoplasmic tRNALeu(CAG) transcripts (G37) are modified by the Mj-Trm5, whereas human cytoplasmic tRNAThr transcripts (A37) are not modified in the presence of Mj-Trm5. Marked decrease in the steady-state levels of mutated tRNAMet Methanocaldococcus jannaschii JCM 10045 ?
-
-
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii NBRC 100440 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii DSM 2661 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii ATCC 43067 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii JAL-1 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii JCM 10045 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 Mj-Trm5
-
 Methanocaldococcus jannaschii
2.1.1.228 TRM5
-
 Methanocaldococcus jannaschii
2.1.1.228 TRMT5
-
 Methanocaldococcus jannaschii
2.1.1.228 tRNA (m1G37)-methyltransferase
-
 Methanocaldococcus jannaschii
2.1.1.228 tRNA methyltransferase 5
-
 Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.228 S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.1.1.228 metabolism a hypertension-associated mitochondrial DNA mutation introduces an m1G37 mutation 4435A->G into human mitochondrial tRNAMet, altering its structure and function, phenotype and pathogenic molecular mechanism, overview. The mutation affects a highly conserved adenosine at position 37, 3' adjacent to the tRNA's anticodon, which is important for the fidelity of codon recognition and stabilization. Defective nucleotide modifications of mitochondrial tRNAs are associated with several human diseases. Trm5 is one of the tRNA (m1G37)-methyltransferases that catalyzes the identical tRNA modification, m1G37 Methanocaldococcus jannaschii