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Literature summary extracted from

  • Fang, L.; Shi, T.; Chen, Y.; Wu, X.; Zhang, C.; Tang, X.; Li, Q.; Hua, R.
    Kinetics and catabolic pathways of the insecticide chlorpyrifos, annotation of the degradation genes, and characterization of enzymes TcpA and Fre in Cupriavidus nantongensis X1T (2019), J. Agric. Food Chem., 67, 2245-2254 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.172 expressed in Escherichia coli BL21(DE3) cells Cupriavidus nantongensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.172 3,5,6-trichloropyridin-2-ol + FADH2 + O2 Cupriavidus nantongensis
-
 3,6-dichloropyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,5,6-trichloropyridin-2-ol + FADH2 + O2 Cupriavidus nantongensis X1T
-
 3,6-dichloropyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,6-dichloropyridine-2,5-diol + FADH2 + O2 Cupriavidus nantongensis
-
 6-chloro-3-hydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,6-dichloropyridine-2,5-diol + FADH2 + O2 Cupriavidus nantongensis X1T
-
 6-chloro-3-hydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 6-chloropyridine-2,3,5-triol + FADH2 + O2 Cupriavidus nantongensis
-
 3,6-dihydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 6-chloropyridine-2,3,5-triol + FADH2 + O2 Cupriavidus nantongensis X1T
-
 3,6-dihydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.172 Cupriavidus nantongensis
-
-
-
1.14.14.172 Cupriavidus nantongensis X1T
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.172 amylose-resin column chromatography Cupriavidus nantongensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.172 3,5,6-trichloropyridin-2-ol + FADH2 + O2
-
 Cupriavidus nantongensis 3,6-dichloropyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,5,6-trichloropyridin-2-ol + FADH2 + O2 86.6% degradation of 100 mg/l 3,5,6-trichloropyridin-2-ol in 24 h Cupriavidus nantongensis 3,6-dichloropyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,5,6-trichloropyridin-2-ol + FADH2 + O2
-
 Cupriavidus nantongensis X1T 3,6-dichloropyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,5,6-trichloropyridin-2-ol + FADH2 + O2 86.6% degradation of 100 mg/l 3,5,6-trichloropyridin-2-ol in 24 h Cupriavidus nantongensis X1T 3,6-dichloropyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,6-dichloropyridine-2,5-diol + FADH2 + O2
-
 Cupriavidus nantongensis 6-chloro-3-hydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 3,6-dichloropyridine-2,5-diol + FADH2 + O2
-
 Cupriavidus nantongensis X1T 6-chloro-3-hydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 6-chloropyridine-2,3,5-triol + FADH2 + O2
-
 Cupriavidus nantongensis 3,6-dihydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?
1.14.14.172 6-chloropyridine-2,3,5-triol + FADH2 + O2
-
 Cupriavidus nantongensis X1T 3,6-dihydroxypyridine-2,5-dione + Cl- + FAD + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.172 ? x * 96000, maltose-binding protein-bound enzyme, SDS-PAGE Cupriavidus nantongensis

Synonyms

EC Number Synonyms Comment Organism
1.14.14.172 2,4,6-trichlorophenol monooxygenase
-
 Cupriavidus nantongensis
1.14.14.172 tcpA
-
 Cupriavidus nantongensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.172 FADH2
-
 Cupriavidus nantongensis