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Literature summary extracted from

  • Hatanaka, T.; Serson, W.; Li, R.; Armstrong, P.; Yu, K.; Pfeiffer, T.; Li, X.L.; Hildebrand, D.
    A Vernonia diacylglycerol acyltransferase can increase renewable oil production (2016), J. Agric. Food Chem., 64, 7188-7194 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.20 biofuel production Vernonia diacylglycerol acyltransferase can increase renewable oil production Vernonia galamensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20 gene DGAT1A, recombinant expression of isozyme AtDGAT1A in Saccharomyces cerevisiae strain INVSc1 microsomes, very low expression level, lipid analyses, overview Arabidopsis thaliana
2.3.1.20 gene DGAT1A, recombinant expression of isozyme ElDGAT1A in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview Euphorbia lagascae
2.3.1.20 gene DGAT1A, recombinant expression of isozyme GmDGAT1A in Saccharomyces cerevisiae strain INVSc1 microsomes, and in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, low expression level, lipid analyses, overview Glycine max
2.3.1.20 gene DGAT1A, recombinant expression of isozyme VgDGAT1A in Saccharomyces cerevisiae strain INVSc1 microsomes, and in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview Vernonia galamensis
2.3.1.20 gene DGAT1B, recombinant expression of isozyme GmDGAT1B in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview Glycine max
2.3.1.20 gene DGAT1B, recombinant expression of isozyme VgDGAT1B in Saccharomyces cerevisiae strain INVSc1 microsomes, and in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview Vernonia galamensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 microsome
-
 Glycine max
-
-
2.3.1.20 microsome
-
 Arabidopsis thaliana
-
-
2.3.1.20 microsome
-
 Vernonia galamensis
-
-
2.3.1.20 microsome
-
 Euphorbia lagascae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Glycine max
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Arabidopsis thaliana
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Vernonia galamensis
-
 CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol Euphorbia lagascae
-
 CoA + 1,2,3-triacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Arabidopsis thaliana Q9SLD2
-
-
2.3.1.20 Euphorbia lagascae T2HV99
-
-
2.3.1.20 Glycine max I1MSF2 cv. Jack
-
2.3.1.20 Glycine max Q5GKZ7 cv. Jack
-
2.3.1.20 Vernonia galamensis B1NM15
-
-
2.3.1.20 Vernonia galamensis B1NM16
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.20 seed
-
 Glycine max
-
2.3.1.20 seed
-
 Arabidopsis thaliana
-
2.3.1.20 seed
-
 Vernonia galamensis
-
2.3.1.20 seed
-
 Euphorbia lagascae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Glycine max CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Arabidopsis thaliana CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Vernonia galamensis CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 acyl-CoA + 1,2-diacyl-sn-glycerol
-
 Euphorbia lagascae CoA + 1,2,3-triacylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Glycine max CoA + 1,2-dioleoyl-3-linoleoylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Arabidopsis thaliana CoA + 1,2-dioleoyl-3-linoleoylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Vernonia galamensis CoA + 1,2-dioleoyl-3-linoleoylglycerol
-
 ?
2.3.1.20 linoleoyl-CoA + 1,2-dioleoyl-sn-glycerol
-
 Euphorbia lagascae CoA + 1,2-dioleoyl-3-linoleoylglycerol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.20 AtDGAT1A
-
 Arabidopsis thaliana
2.3.1.20 DGAT
-
 Glycine max
2.3.1.20 DGAT
-
 Arabidopsis thaliana
2.3.1.20 DGAT
-
 Vernonia galamensis
2.3.1.20 DGAT
-
 Euphorbia lagascae
2.3.1.20 diacylglycerol acyltransferase
-
 Glycine max
2.3.1.20 diacylglycerol acyltransferase
-
 Arabidopsis thaliana
2.3.1.20 diacylglycerol acyltransferase
-
 Vernonia galamensis
2.3.1.20 diacylglycerol acyltransferase
-
 Euphorbia lagascae
2.3.1.20 ElDGAT1A
-
 Euphorbia lagascae
2.3.1.20 GmDGAT1A
-
 Glycine max
2.3.1.20 GmDGAT1B
-
 Glycine max
2.3.1.20 VgDGAT1A
-
 Vernonia galamensis
2.3.1.20 VgDGAT1B
-
 Vernonia galamensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.20 30
-
 assay at Glycine max
2.3.1.20 30
-
 assay at Arabidopsis thaliana
2.3.1.20 30
-
 assay at Vernonia galamensis
2.3.1.20 30
-
 assay at Euphorbia lagascae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 7.1
-
 assay at Glycine max
2.3.1.20 7.1
-
 assay at Arabidopsis thaliana
2.3.1.20 7.1
-
 assay at Vernonia galamensis
2.3.1.20 7.1
-
 assay at Euphorbia lagascae

General Information

EC Number General Information Comment Organism
2.3.1.20 additional information fatty acid composition of high-oil lines and cultivar Jack wild-type soybean seed oil, overview Glycine max