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Literature summary extracted from

  • Kawamura, T.; Anraku, R.; Hasegawa, T.; Tomikawa, C.; Hori, H.
    Transfer RNA methyltransferases from Thermoplasma acidophilum, a thermoacidophilic archaeon (2015), Int. J. Mol. Sci., 16, 91-113 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.206 gene Ta0931, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Rosetta 2 Thermoplasma acidophilum
2.1.1.216 gene Ta0997, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21 (DE3) Rosetta 2 Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.206 additional information Thermoplasma acidophilum purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed ?
-
-
2.1.1.206 additional information Thermoplasma acidophilum JCM 9062 purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed ?
-
-
2.1.1.206 additional information Thermoplasma acidophilum AMRC-C165 purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed ?
-
-
2.1.1.206 additional information Thermoplasma acidophilum ATCC 25905 purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed ?
-
-
2.1.1.206 additional information Thermoplasma acidophilum NBRC 15155 purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed ?
-
-
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet Thermoplasma acidophilum mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet Thermoplasma acidophilum JCM 9062 mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet Thermoplasma acidophilum AMRC-C165 mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet Thermoplasma acidophilum ATCC 25905 mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet Thermoplasma acidophilum NBRC 15155 mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet Thermoplasma acidophilum initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet Thermoplasma acidophilum JCM 9062 initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet Thermoplasma acidophilum AMRC-C165 initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet Thermoplasma acidophilum ATCC 25905 initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet Thermoplasma acidophilum NBRC 15155 initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA Thermoplasma acidophilum JCM 9062
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA Thermoplasma acidophilum AMRC-C165
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA Thermoplasma acidophilum ATCC 25905
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA Thermoplasma acidophilum NBRC 15155
-
 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) Thermoplasma acidophilum the methylated nucleotide is identified as pCm S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) Thermoplasma acidophilum JCM 9062 the methylated nucleotide is identified as pCm S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) Thermoplasma acidophilum AMRC-C165 the methylated nucleotide is identified as pCm S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) Thermoplasma acidophilum ATCC 25905 the methylated nucleotide is identified as pCm S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) Thermoplasma acidophilum NBRC 15155 the methylated nucleotide is identified as pCm S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum JCM 9062
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum AMRC-C165
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum ATCC 25905
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum NBRC 15155
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum JCM 9062
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum AMRC-C165
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum ATCC 25905
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG) Thermoplasma acidophilum NBRC 15155
-
 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 S-adenosyl-L-methionine + 2'-O-methylguanine26 in elongator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in elongator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + 2'-O-methylguanine26 in initiator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in initiator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in elongator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in initiator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum JCM 9062
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum AMRC-C165
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum ATCC 25905
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA Thermoplasma acidophilum NBRC 15155
-
 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum JCM 9062
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum AMRC-C165
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum ATCC 25905
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA Thermoplasma acidophilum NBRC 15155
-
 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.206 Thermoplasma acidophilum Q9HJN6
-
-
2.1.1.206 Thermoplasma acidophilum AMRC-C165 Q9HJN6
-
-
2.1.1.206 Thermoplasma acidophilum ATCC 25905 Q9HJN6
-
-
2.1.1.206 Thermoplasma acidophilum JCM 9062 Q9HJN6
-
-
2.1.1.206 Thermoplasma acidophilum NBRC 15155 Q9HJN6
-
-
2.1.1.216 Thermoplasma acidophilum P57706
-
-
2.1.1.216 Thermoplasma acidophilum AMRC-C165 P57706
-
-
2.1.1.216 Thermoplasma acidophilum ATCC 25905 P57706
-
-
2.1.1.216 Thermoplasma acidophilum JCM 9062 P57706
-
-
2.1.1.216 Thermoplasma acidophilum NBRC 15155 P57706
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.206 recombinant enzyme from Escherichia coli strain BL21 (DE3) Rosetta 2 by anion exchange chromatography, heparin affinity chromatography, and gel filtration, followed by dialysis and ultrafiltration Thermoplasma acidophilum
2.1.1.216 recombinant enzyme from Escherichia coli strain BL21 (DE3) Rosetta 2 Thermoplasma acidophilum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.206 additional information Thermoplasma acidophilum grows optimally at 59°C and pH 1.9 Thermoplasma acidophilum
-
2.1.1.216 additional information Thermoplasma acidophilum grows optimally at 59°C and pH 1.9 Thermoplasma acidophilum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.206 additional information purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed Thermoplasma acidophilum ?
-
-
2.1.1.206 additional information purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed Thermoplasma acidophilum JCM 9062 ?
-
-
2.1.1.206 additional information purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed Thermoplasma acidophilum AMRC-C165 ?
-
-
2.1.1.206 additional information purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed Thermoplasma acidophilum ATCC 25905 ?
-
-
2.1.1.206 additional information purified Trm56 can methylate the precursor of elongator tRNAMet, which has an intron at the canonical position. But the speed of methyl-transfer by Trm56 to the precursor RNA is considerably slower than that to the mature transcript, which suggests that Trm56 acts mainly on the transcript after the intron has been removed Thermoplasma acidophilum NBRC 15155 ?
-
-
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in elongator tRNAMet mature elongator tRNAMet is a better substrate that the precursor of elongator tRNA, which has an intron at the canonical position Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in elongator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in initiator tRNAMet initiator tRNAMet from Thermoplasma acidophilum strain HO-62 contains G+, m1I, and m22G Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in initiator tRNAMet
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA
-
 Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNA
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) the methylated nucleotide is identified as pCm Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) the methylated nucleotide is identified as pCm Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) the methylated nucleotide is identified as pCm Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) the methylated nucleotide is identified as pCm Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.206 S-adenosyl-L-methionine + cytidine56 in tRNALeu(UAG) the methylated nucleotide is identified as pCm Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + 2'-O-methylcytidine56 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum JCM 9062 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum JCM 9062 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum AMRC-C165 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum ATCC 25905 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 2 S-adenosyl-L-methionine + guanine26 in tRNALeu(UAG)
-
 Thermoplasma acidophilum NBRC 15155 2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeu(UAG)
-
 ?
2.1.1.216 additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum ?
-
-
2.1.1.216 additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum JCM 9062 ?
-
-
2.1.1.216 additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum AMRC-C165 ?
-
-
2.1.1.216 additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum ATCC 25905 ?
-
-
2.1.1.216 additional information Ta0997 gene product is a single site-specific Trm1 Thermoplasma acidophilum NBRC 15155 ?
-
-
2.1.1.216 S-adenosyl-L-methionine + 2'-O-methylguanine26 in elongator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in elongator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + 2'-O-methylguanine26 in initiator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-dimethylguanine26 in initiator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in elongator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in elongator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in initiator tRNAMet
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + 2'-O-methylguanine26 in initiator tRNAMet
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + guanine26 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + N2-methylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum JCM 9062 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum AMRC-C165 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum ATCC 25905 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
2.1.1.216 S-adenosyl-L-methionine + N2-methylguanine26 in tRNA
-
 Thermoplasma acidophilum NBRC 15155 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.206 Ta0931
-
 Thermoplasma acidophilum
2.1.1.206 transfer RNA methyltransferase
-
 Thermoplasma acidophilum
2.1.1.206 Trm56
-
 Thermoplasma acidophilum
2.1.1.216 Ta0997
-
 Thermoplasma acidophilum
2.1.1.216 transfer RNA methyltransferase
-
 Thermoplasma acidophilum
2.1.1.216 Trm1
-
 Thermoplasma acidophilum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.206 50
-
 assay at Thermoplasma acidophilum
2.1.1.216 50
-
 assay at Thermoplasma acidophilum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.206 7.6
-
 assay at Thermoplasma acidophilum
2.1.1.216 7.6
-
 assay at Thermoplasma acidophilum

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.206 S-adenosyl-L-methionine
-
 Thermoplasma acidophilum
2.1.1.216 S-adenosyl-L-methionine
-
 Thermoplasma acidophilum

General Information

EC Number General Information Comment Organism
2.1.1.206 additional information Trm56 and an yet unknown tRNA (m7G49) methyltransferase might be included in protein complexes and precipitated by centrifugation at 30000 × g. Thermoplasma acidophilum Trm56 might be part of a large complex with other proteins in living cells Thermoplasma acidophilum
2.1.1.206 physiological function the enzyme Trm56 is responsible for the typical archaeal tRNA modification of 2'-O-methylcytidine at position 56 (Cm56). The Cm modification is only found at position 56 in native tRNALeuUAG. Trm56 methylates both the elongator tRNAMet transcript and its precursor. But the methyl group acceptance activity of the precursor is considerably lower than that of the mature transcript, the methylation by Trm56 occurs mainly after the removal of the intron Thermoplasma acidophilum
2.1.1.216 evolution Trm1 enzymes can be divided into two types on the basis of their specificity for the target guanosine(s). One is a single-site-specific, which modifies only G26 and is found in eukaryotes and archaea. The second is a multi-site-specific Trm1, which modifies both G26 and G27 and is found in the hyperthermophilic eubacterium, Aquifex aeolicus Thermoplasma acidophilum
2.1.1.216 physiological function the Ta0997 gene product methylates the tRNALeuUAG transcript and 14C-nucleotide analysis reveals that the modified nucleotide is pm22G. Archaeal Trm1 can methylate the tRNALeuUAG transcript, which has a long variable region. In the case of class I tRNAs, archaeal Trm1 is reported to recognize the D-stem and the size of the variable region. Therefore, archaeal Trm1 might be able to recognize the large variable region in the class II tRNAs. Trm1 efficiently methylates both the mature elongator tRNAMet transcript and the precursor with an intron. Archaeal Trm1 does not recognize the anticodon loop. The G modification at position 26 is a mixture of m2G and m22G, which is formed by Trm1 Thermoplasma acidophilum