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Literature summary extracted from

  • Giardina, G.; Brunotti, P.; Fiascarelli, A.; Cicalini, A.; Costa, M.G.; Buckle, A.M.; di Salvo, M.L.; Giorgi, A.; Marani, M.; Paone, A.; Rinaldo, S.; Paiardini, A.; Contestabile, R.; Cutruzzola, F.
    How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state (2015), FEBS J., 282, 1225-1241 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.2.1 holo- and apo-isoform SHMT2, hanging drop vapor diffusion method, using 0.1 M Bis/Tris propane pH 7.5, 5% (v/v) glycerol, 12-16% (w/v) poly(ethylene glycol) 3350, 0.2 M potassium fluoride Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.02
-
 tetrahydrofolate apparent value, isoform SHMT1, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 0.023
-
 tetrahydrofolate apparent value, isoform SHMT2, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 0.1
-
 L-serine apparent value, isoform SHMT1, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 0.278
-
 L-serine apparent value, isoform SHMT2, at pH 7.2 and 20°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol isoform SHMT1 Homo sapiens 5829
-
2.1.2.1 mitochondrion isoform SHMT2 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
 tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine Homo sapiens
-
 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Homo sapiens P34896
-
-
2.1.2.1 Homo sapiens P34897
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
 Homo sapiens tetrahydrofolate + L-serine
-
 r
2.1.2.1 tetrahydrofolate + L-serine
-
 Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer or tetramer isoform SHMT2, X-ray crystallography Homo sapiens
2.1.2.1 tetramer isoform SHMT1, X-ray crystallography Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
 Homo sapiens
2.1.2.1 SHMT1 isoform Homo sapiens
2.1.2.1 SHMT2 isoform Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 9.58
-
 tetrahydrofolate isoform SHMT1, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 9.58
-
 tetrahydrofolate isoform SHMT2, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 13.13
-
 L-serine isoform SHMT1, at pH 7.2 and 20°C Homo sapiens
2.1.2.1 13.13
-
 L-serine isoform SHMT2, at pH 7.2 and 20°C Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
 Homo sapiens