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Literature summary extracted from
- Coexpression of 5-aminolevulinic acid synthase gene facilitates heterologous production of thermostable cytochrome P450, CYP119, in holo form in Escherichia coli (2018), ChemBioChem, 19, 2156-2159 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.14.13.B28 | synthesis | coexpression of 5-aminolevulinic acid synthase (ALAS) from Rhodobacter capsulatus improves the heterologous production of CYP119. Coexpression of ALAS increases the amount of heterologous CYP119 isolated and the ratio of its holo form. The ratio of holo-CYP119 resulting from the coexpression of ALAS in Escherichia coli is 99%, whereas that from cells expressing CYP119 exclusively is 66% | Sulfolobus acidocaldarius |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.37 | expressed in Escherichia coli Rosetta 2 (DE3) cells | Rhodobacter capsulatus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.37 | succinyl-CoA + glycine | Rhodobacter capsulatus | - |
5-aminolevulinate + CoA + CO2 | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.B28 | Sulfolobus acidocaldarius | Q55080 | - |
- |
| 1.14.13.B28 | Sulfolobus acidocaldarius ATCC 33909 | Q55080 | - |
- |
| 2.3.1.37 | Rhodobacter capsulatus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.37 | succinyl-CoA + glycine | - |
Rhodobacter capsulatus | 5-aminolevulinate + CoA + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.3.1.37 | ? | x * 43600, calculated from amino acid sequence | Rhodobacter capsulatus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.B28 | CYP119 | - |
Sulfolobus acidocaldarius |
| 2.3.1.37 | 5-aminolevulinic acid synthase | - |
Rhodobacter capsulatus |
| 2.3.1.37 | ALAS | - |
Rhodobacter capsulatus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.37 | pyridoxal 5'-phosphate | - |
Rhodobacter capsulatus | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.3.1.37 | physiological function | coexpression of the enzyme improves the heterologous production of cytochrome P450, CYP119, from Sulfolobus acidocaldarius in Escherichia coli | Rhodobacter capsulatus |
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