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Literature summary extracted from

  • Vit, A.; Misson, L.; Blankenfeldt, W.; Seebeck, F.
    Ergothioneine biosynthetic methyltransferase EgtD reveals the structural basis of aromatic amino acid betaine biosynthesis (2015), ChemBioChem, 16, 119-125 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.44 structure of EgtD as an apoprotein (1.75 A resolution), as a binary complex with N-alpha-dimethylhistidine (1.9 A), and as a ternary complex with N-alpha-dimethylhistidine and SAH (1.5 A) Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.44 E285A active site mutation, change the substrate specificity and transforms the histidine-methyltransferase towards a tryptophan-methyltransferase Mycolicibacterium smegmatis
2.1.1.44 M252A/E285A active site mutation, change the substrate specificity and transforms the histidine-methyltransferase towards a tryptophan-methyltransferase Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.44 3 S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis
-
 3 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 3 S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis ATCC 700084
-
 3 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis
-
 S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-histidine Mycolicibacterium smegmatis ATCC 700084
-
 S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine Mycolicibacterium smegmatis
-
 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine Mycolicibacterium smegmatis ATCC 700084
-
 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha-methyl-L-histidine Mycolicibacterium smegmatis
-
 S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha-methyl-L-histidine Mycolicibacterium smegmatis ATCC 700084
-
 S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.44 Mycolicibacterium smegmatis A0R5M8
-
-
2.1.1.44 Mycolicibacterium smegmatis ATCC 700084 A0R5M8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.44 3 S-adenosyl-L-methionine + L-histidine
-
 Mycolicibacterium smegmatis 3 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 3 S-adenosyl-L-methionine + L-histidine
-
 Mycolicibacterium smegmatis ATCC 700084 3 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 additional information EgtD is a four- and 70-times stronger binder of Nalpha-methylhistidine and N-alpha-dimethylhistidine, respectively, than of histidine Mycolicibacterium smegmatis ?
-
-
2.1.1.44 additional information EgtD is a four- and 70-times stronger binder of Nalpha-methylhistidine and N-alpha-dimethylhistidine, respectively, than of histidine Mycolicibacterium smegmatis ATCC 700084 ?
-
-
2.1.1.44 S-adenosyl-L-methionine + DOPA poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-histidine
-
 Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-histidine
-
 Mycolicibacterium smegmatis ATCC 700084 S-adenosyl-L-homocysteine + Nalpha-methyl-L-histidine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-phenylalanine poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-tryptophan poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.44 S-adenosyl-L-methionine + L-tyrosine poor substrate Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + ?
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine
-
 Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha,Nalpha-dimethyl-L-histidine
-
 Mycolicibacterium smegmatis ATCC 700084 S-adenosyl-L-homocysteine + hercynine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha-methyl-L-histidine
-
 Mycolicibacterium smegmatis S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
 ?
2.1.1.44 S-adenosyl-L-methionine + Nalpha-methyl-L-histidine
-
 Mycolicibacterium smegmatis ATCC 700084 S-adenosyl-L-homocysteine + Nalpha,Nalpha-dimethyl-L-histidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.44 EgtD
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.44 0.1
-
 L-histidine mutant E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.23
-
 Nalpha-methyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.43
-
 Nalpha,Nalpha-dimethyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.58
-
 L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.44 0.0011
-
 L-tyrosine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.0019
-
 L-histidine mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.002
-
 L-tryptophan wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.0024
-
 L-phenylalanine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.017
-
 L-tyrosine mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.04
-
 L-histidine mutant E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.1
-
 L-phenylalanine mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 0.4
-
 L-tryptophan mutant E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 5.3
-
 L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 5.5
-
 L-tryptophan mutant M252A/E285A, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 13
-
 Nalpha-methyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis
2.1.1.44 17
-
 Nalpha,Nalpha-dimethyl-L-histidine wild-type, pH 8.0, 26°C Mycolicibacterium smegmatis