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Literature summary extracted from

  • Munshi, M.; Gardin, J.; Singh, A.; Luberto, C.; Rieger, R.; Bouklas, T.; Fries, B.; Del Poeta, M.
    The role of ceramide synthases in the pathogenicity of Cryptococcus neoformans (2018), Cell Rep., 22, 1392-1400 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.24 expression in Escherichia coli Cryptococcus neoformans var. grubii

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.24 Cryptococcus neoformans var. grubii J9VFF7 isoform Cer1; serotype A
-
2.3.1.24 Cryptococcus neoformans var. grubii J9VMM2 isoform Cer2; serotype A
-
2.3.1.24 Cryptococcus neoformans var. grubii J9VQN5 isoform Cer3; serotype A
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.24 additional information isoform Cer1 shows a clear preference for C18 fatty acyl CoA and sphingosine as a substrate Cryptococcus neoformans var. grubii ?
-
-
2.3.1.24 stearoyl-CoA + phytosphingosine
-
 Cryptococcus neoformans var. grubii CoA + N-stearoylphytosphingosine
-
 ?
2.3.1.24 stearoyl-CoA + sphingosine
-
 Cryptococcus neoformans var. grubii CoA + N-stearoylsphingosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.24 CER2
-
 Cryptococcus neoformans var. grubii
2.3.1.24 Cer3
-
 Cryptococcus neoformans var. grubii
2.3.1.24 CNAG_02086
-
 Cryptococcus neoformans var. grubii
2.3.1.24 CNAG_02087
-
 Cryptococcus neoformans var. grubii
2.3.1.24 CNAG_06717
-
 Cryptococcus neoformans var. grubii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.24 35
-
-
 Cryptococcus neoformans var. grubii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.24 7
-
-
 Cryptococcus neoformans var. grubii

General Information

EC Number General Information Comment Organism
2.3.1.24 metabolism the production of ceramide isoforms is regulated by the stoichiometry of the three ceramide synthase isoform Cer1, Cer2, Cer3 of Cryptococcus neoformans in the presence of different substrate chain lengths. When Cer1 and Cer2 are coexpressed, C24 phytoceramide is the major product. When Cer1 and Cer3 are co-expressed, the enzyme activity shifts to C18 and C26 fatty acyl CoA products Cryptococcus neoformans var. grubii
2.3.1.24 physiological function the average survival of mice infected with wild-type Cryptococcus neoformans is about 25 days, infection with a Cer2 deletion strain causes aout 70% mortality. The lipid profile of the deletion strain is perturbed from wild-type. The deletion strain shows little to no depletion of lipids but a marked increase in dihydrosphingosine, C18 dihydroceramide, and total short-chain GlcCeramides as well as abundance of most C18 lipids in the inositolphosphoryl ceramide pathway Cryptococcus neoformans var. grubii
2.3.1.24 physiological function the average survival of mice infected with wild-type Cryptococcus neoformans is about 25 days, infection with a Cer3 deletion strain causes aout 70% mortality. The lipid profile of the deletion strain is perturbed from wild-type. The deletion strain shows significant depletion of C26 phytoceramides in acidic conditions and a decrease in lipids along the GlcCer branch of the pathway as well as a decrease in C24 and C26 lipids in the inositol phosphorylceramide pathway under alkaline conditions Cryptococcus neoformans var. grubii
2.3.1.24 physiological function the average survival of mice infected with wild-type Cryptococcus neoformans is about 25 days, while all mice infected with a Cer1 deletion strain survive. The deletion strain cells are not observed to progress to the brain of infected mice. The lipid profile of the deletion strain is significantly perturbed from wild-type. Cer1 is the major ceramide synthase responsible for using C18 fatty acyl CoA to generate C18 ceramides. Deletion strain cells have cell division defects leading to the development of elongated cells with a smaller capsule, they show gross morphological defects and inability to complete cytokinesis Cryptococcus neoformans var. grubii