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Literature summary extracted from

  • Tan, Z.; Zhao, J.; Chen, J.; Rao, D.; Zhou, W.; Chen, N.; Zheng, P.; Sun, J.; Ma, Y.
    Enhancing thermostability and removing hemin inhibition of Rhodopseudomonas palustris 5-aminolevulinic acid synthase by computer-aided rational design (2019), Biotechnol. Lett., 41, 181-191 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.37 expressed in Escherichia coli BL21(DE3) cells and Corynebacterium glutamicum strain ATCC 13032 Rhodopseudomonas palustris

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 H15K the mutant exhibits a 6.0°C higher melting temperature and shows a 10.3fold increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H15R the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H176D the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H206F the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H206Y the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H216D inactive Rhodopseudomonas palustris
2.3.1.37 H236D the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H293Y the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H29R the mutant exhibits a 2.3°C higher melting temperature and shows a 6.7fold increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H304M the mutant shows a strong increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H304Q the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H304V the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H322L the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H322V the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H385R the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H39Y the mutant shows an increase in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H61D the mutant shows a severe decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris
2.3.1.37 H61N the mutant shows a decrease in specific activity after 1 h incubation at 37°C compared to the wild type enzyme Rhodopseudomonas palustris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.37 hemin 27% residual activity at 0.01 mM Rhodopseudomonas palustris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.01513
-
 succinyl-CoA mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 0.0214
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 0.02227
-
 succinyl-CoA mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 2.31
-
 glycine wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 2.63
-
 glycine mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 3.25
-
 glycine mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine Rhodopseudomonas palustris
-
 5-aminolevulinate + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Rhodopseudomonas palustris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.37 His-Trap column chromatography Rhodopseudomonas palustris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Rhodopseudomonas palustris 5-aminolevulinate + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.37 ALA synthase
-
 Rhodopseudomonas palustris
2.3.1.37 ALAS
-
 Rhodopseudomonas palustris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.37 37
-
 after 60 min incubation at 37°C, the residual activity of wild type dramatically drops to 8% of the original activity Rhodopseudomonas palustris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 2.74
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 3.1
-
 succinyl-CoA mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 3.2
-
 succinyl-CoA mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate
-
 Rhodopseudomonas palustris

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.37 0.88
-
 glycine mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 1.18
-
 glycine wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 1.22
-
 glycine mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 130
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 140
-
 succinyl-CoA mutant enzyme H29R, at pH 7.5 and 37°C Rhodopseudomonas palustris
2.3.1.37 230
-
 succinyl-CoA mutant enzyme H15K, at pH 7.5 and 37°C Rhodopseudomonas palustris