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Literature summary extracted from

  • Gutmann, A.; Lepak, A.; Diricks, M.; Desmet, T.; Nidetzky, B.
    Glycosyltransferase cascades for natural product glycosylation Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP (2017), Biotechnol. J., 12, 1600557 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 expressed in Escherichia coli BL21-Gold (DE3) cells Glycine max
2.4.1.13 expressed in Escherichia coli BL21-Gold (DE3) cells Acidithiobacillus caldus

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.13 L637M/T640V the double mutant shows a 60fold reduced KM value for UDP and substantially improved UDP-glucose recycling. The mutant is roughly 8times more active than the wild type enzyme Acidithiobacillus caldus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Mg2+ 13 mM used in assay conditions Glycine max
2.4.1.13 Mg2+ 13 mM used in assay conditions Acidithiobacillus caldus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.13 NDP-alpha-D-glucose + D-fructose Glycine max
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-alpha-D-glucose + D-fructose Acidithiobacillus caldus
-
 NDP + sucrose
-
 r
2.4.1.13 NDP-alpha-D-glucose + D-fructose Acidithiobacillus caldus ATCC 51756
-
 NDP + sucrose
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Acidithiobacillus caldus A0A059ZV61
-
-
2.4.1.13 Acidithiobacillus caldus ATCC 51756 A0A059ZV61
-
-
2.4.1.13 Glycine max P13708
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13 His tag affinity chromatography Acidithiobacillus caldus
2.4.1.13 Strep tag affinity chromatography Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 NDP-alpha-D-glucose + D-fructose
-
 Glycine max NDP + sucrose
-
 r
2.4.1.13 NDP-alpha-D-glucose + D-fructose
-
 Acidithiobacillus caldus NDP + sucrose
-
 r
2.4.1.13 NDP-alpha-D-glucose + D-fructose
-
 Acidithiobacillus caldus ATCC 51756 NDP + sucrose
-
 r
2.4.1.13 UDP + sucrose
-
 Glycine max UDP-alpha-D-glucose + D-fructose
-
 r
2.4.1.13 UDP + sucrose
-
 Acidithiobacillus caldus UDP-alpha-D-glucose + D-fructose
-
 r
2.4.1.13 UDP + sucrose
-
 Acidithiobacillus caldus ATCC 51756 UDP-alpha-D-glucose + D-fructose
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.4.1.13 SuSy
-
 Glycine max
2.4.1.13 SuSy
-
 Acidithiobacillus caldus