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Literature summary extracted from

  • Stojanovski, B.M.; Breydo, L.; Uversky, V.N.; Ferreira, G.C.
    Murine erythroid 5-aminolevulinate synthase Truncation of a disordered N-terminal extension is not detrimental for catalysis (2016), Biochim. Biophys. Acta, 1864, 441-452 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 additional information the truncation of the N-terminal region in mutant DELTAmALAS2 does not affect the Km values for either substrate while the kcat of the mutant enzyme, relative to that of the wild type enzyme, is increased by 22% Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 1
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Mus musculus
2.3.1.37 1.3
-
 succinyl-CoA mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus
2.3.1.37 8
-
 glycine wild type enzyme, at pH 7.5 and 37°C Mus musculus
2.3.1.37 11
-
 glycine mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine Mus musculus
-
 5-aminolevulinate + CoA + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Mus musculus P08680
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.37 erythroid cell
-
 Mus musculus
-
2.3.1.37 peripheral blood cell
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Mus musculus 5-aminolevulinate + CoA + CO2
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.37 homodimer 2 * 52000, 36 N-terminal amino acid-truncated enzyme, SDS-PAGE Mus musculus
2.3.1.37 homodimer 2 * 56000, native enzyme, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.37 ALAS2 isoform Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.37 49.7
-
 melting temperature Mus musculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 0.25
-
 glycine wild type enzyme, at pH 7.5 and 37°C Mus musculus
2.3.1.37 0.32
-
 glycine mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate
-
 Mus musculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.37 0.0291
-
 glycine mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus
2.3.1.37 0.0312
-
 glycine wild type enzyme, at pH 7.5 and 37°C Mus musculus
2.3.1.37 246
-
 succinyl-CoA mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C Mus musculus
2.3.1.37 250
-
 succinyl-CoA wild type enzyme, at pH 7.5 and 37°C Mus musculus