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Literature summary extracted from

  • Kawana, H.; Kano, K.; Shindou, H.; Inoue, A.; Shimizu, T.; Aoki, J.
    An accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases (2019), Biochim. Biophys. Acta, 1864, 1053-1060 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.23 gene LCLAT1, recombinant expression in HEK-293 cells Mus musculus
2.3.1.23 gene LPCAT1, functional recombinant expression in HEK-293 cells Mus musculus
2.3.1.23 gene LPCAT1, functional recombinant expression in HEK-293 cells Homo sapiens
2.3.1.23 gene LPCAT3, recombinant expression in HEK-293 cells Mus musculus
2.3.1.62 gene LCLAT1, recombinant expression in HEK-293 cells Mus musculus
2.3.1.62 gene LPCAT1, functional recombinant expression in HEK-293 cells Mus musculus
2.3.1.62 gene LPCAT1, functional recombinant expression in HEK-293 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.23 membrane
-
 Mus musculus 16020
-
2.3.1.23 membrane
-
 Homo sapiens 16020
-
2.3.1.62 membrane
-
 Mus musculus 16020
-
2.3.1.62 membrane
-
 Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylcholine Mus musculus
-
 CoA + 1,2-diacyl-lysophosphatidylcholine
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-sn-lysophosphatidylcholine Mus musculus
-
 CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-sn-lysophosphatidylcholine Homo sapiens
-
 CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 acyl-CoA + 2-acyl-sn-lysophosphatidylglycerol Mus musculus
-
 CoA + 1,2-diacyl-sn-lysophosphatidylglycerol
-
 ?
2.3.1.23 additional information Mus musculus LPCAT3 is critical for introduction of polyunsaturated fatty acids (PUFAs), especially arachidonic acid (20:4) at the sn-2 position of various LPLs, generating various types of arachidonoyl phospholipids ?
-
-
2.3.1.23 additional information Mus musculus LYCAT introduces stearic acid (18:0) selectively at the sn-1 position of lysophosphatidylinositol (LPI). The enzyme also catalyzes the reacylation of lysocardiolipin to cardiolipin ?
-
-
2.3.1.62 acyl-CoA + 2-acyl-sn-glycero-3-phosphatidylcholine Mus musculus
-
 CoA + 1,2-diacyl-sn-glycero-3-phosphatidylcholine
-
 ?
2.3.1.62 acyl-CoA + 2-acyl-sn-lysophosphatidylcholine Mus musculus
-
 CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 acyl-CoA + 2-acyl-sn-lysophosphatidylcholine Homo sapiens
-
 CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 additional information Mus musculus LYCAT introduces stearic acid (18:0) selectively at the sn-1 position of lysophosphatidylinositol (LPI). The enzyme also catalyzes the reacylation of lysocardiolipin to cardiolipin ?
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.23 Homo sapiens Q8NF37
-
-
2.3.1.23 Mus musculus Q3TFD2
-
-
2.3.1.23 Mus musculus Q3UN02
-
-
2.3.1.23 Mus musculus Q91V01
-
-
2.3.1.62 Homo sapiens Q8NF37
-
-
2.3.1.62 Mus musculus Q3TFD2
-
-
2.3.1.62 Mus musculus Q3UN02
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.23 HEK-293A cell
-
 Mus musculus
-
2.3.1.23 HEK-293A cell
-
 Homo sapiens
-
2.3.1.62 HEK-293A cell
-
 Mus musculus
-
2.3.1.62 HEK-293A cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.23 acyl-CoA + 1-acyl-lysophosphatidylcholine
-
 Mus musculus CoA + 1,2-diacyl-lysophosphatidylcholine
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 acyl-CoA + 1-acyl-sn-lysophosphatidylcholine
-
 Homo sapiens CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 acyl-CoA + 2-acyl-sn-lysophosphatidylglycerol
-
 Mus musculus CoA + 1,2-diacyl-sn-lysophosphatidylglycerol
-
 ?
2.3.1.23 arachidonoyl-CoA + 1-stearoyl-lysophosphatidylcholine preferred reaction Mus musculus CoA + 1-stearoyl-2-arachidonoyl-lysophosphatidylcholine
-
 ?
2.3.1.23 arachidonoyl-CoA + 2-stearoyl-lysophosphatidylcholine low activity, cf. EC 2.3.1.62 Mus musculus CoA + 1-arachidonoyl-2-stearoyl-lysophosphatidylcholine
-
 ?
2.3.1.23 additional information LPCAT3 is critical for introduction of polyunsaturated fatty acids (PUFAs), especially arachidonic acid (20:4) at the sn-2 position of various LPLs, generating various types of arachidonoyl phospholipids Mus musculus ?
-
-
2.3.1.23 additional information LYCAT introduces stearic acid (18:0) selectively at the sn-1 position of lysophosphatidylinositol (LPI). The enzyme also catalyzes the reacylation of lysocardiolipin to cardiolipin Mus musculus ?
-
-
2.3.1.23 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LPCAT1 introduces palmitic acid both at the sn-1 and sn-2 positions of palmitoyl-LPC Mus musculus ?
-
-
2.3.1.23 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LPCAT1 introduces palmitic acid both at the sn-1 and sn-2 positions of palmitoyl-lysophosphatidylcholine (LPC). sn-1-Palmitoyl LPC appears to have a slightly higher affinity for LPCAT1 than sn-2-palmitoyl LPC, while sn-2-palmitoyl LPC has a higher turnover rate Homo sapiens ?
-
-
2.3.1.23 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LPCAT3 introduces arachidonic acid predominantly at the sn-2 position of lysophosphatidylcholine (LPC) Mus musculus ?
-
-
2.3.1.23 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LYCAT introduces stearic acid predominantly at the sn-1 position of LPG. The enzyme also catalyzes the reacylation of lysocardiolipin to cardiolipin Mus musculus ?
-
-
2.3.1.23 palmitoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 palmitoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine
-
 Homo sapiens CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 palmitoyl-CoA + 2-palmitoyl-lysophosphatidylcholine reaction of EC 2.3.1.62 Homo sapiens CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 palmitoyl-CoA + 2-palmitoyl-sn-lysophosphatidylcholine reaction of EC 2.3.1.62 Mus musculus CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.23 stearoyl-CoA + 1-oleoyl-sn-lysophosphatidylglycerol LYCAT introduces stearic acid predominantly at the sn-1 position of lysophosphatidylglycerol (LPG), cf. EC 2.3.1.62 Mus musculus CoA + 1-oleoyl-2-stearoyl-sn-lysophosphatidylglycerol
-
 ?
2.3.1.62 acyl-CoA + 2-acyl-sn-glycero-3-phosphatidylcholine
-
 Mus musculus CoA + 1,2-diacyl-sn-glycero-3-phosphatidylcholine
-
 ?
2.3.1.62 acyl-CoA + 2-acyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 acyl-CoA + 2-acyl-sn-lysophosphatidylcholine
-
 Homo sapiens CoA + 1,2-diacyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 additional information LYCAT introduces stearic acid (18:0) selectively at the sn-1 position of lysophosphatidylinositol (LPI). The enzyme also catalyzes the reacylation of lysocardiolipin to cardiolipin Mus musculus ?
-
-
2.3.1.62 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LPCAT1 introduces palmitic acid both at the sn-1 and sn2 positions of palmitoyl-LPC Mus musculus ?
-
-
2.3.1.62 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LPCAT1 introduces palmitic acid both at the sn-1 and sn2 positions of palmitoyl-lysophosphatidylcholine (LPC). sn-1-palmitoyl LPC appears to have a slightly higher affinity for LPCAT1 than sn-2-palmitoyl LPC, while sn-2-palmitoyl LPC has a higher turnover rate Homo sapiens ?
-
-
2.3.1.62 additional information development and evaluation of an accurate and versatile method for determining the acyl group-introducing position of lysophospholipid acyltransferases, overview. Positional specificity of the hydroxyl group of the glycerol backbone (sn-2 or sn-1) at which LPLATs introduce a fatty acid. LYCAT introduces stearic acid predominantly at the sn-1 position of LPG. The enzyme also catalyzes the reacylation of lysocardiolipin to cardiolipin Mus musculus ?
-
-
2.3.1.62 palmitoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine reaction of EC 2.3.1.23 Mus musculus CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 palmitoyl-CoA + 1-palmitoyl-sn-lysophosphatidylcholine reaction of EC 2.3.1.23 Homo sapiens CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 palmitoyl-CoA + 2-palmitoyl-lysophosphatidylcholine
-
 Homo sapiens CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 palmitoyl-CoA + 2-palmitoyl-sn-lysophosphatidylcholine
-
 Mus musculus CoA + 1,2-dipalmitoyl-sn-lysophosphatidylcholine
-
 ?
2.3.1.62 stearoyl-CoA + 2-oleoyl-sn-glycero-3-phosphatidylcholine preferred substrate Mus musculus CoA + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.23 1-acylglycerol-3-phosphate O-acyltransferase 8 UniProt Mus musculus
2.3.1.23 1-AGP acyltransferase 8 UniProt Mus musculus
2.3.1.23 1-AGPAT 8 UniProt Mus musculus
2.3.1.23 acyl-CoA:lysocardiolipin acyltransferase 1
-
 Mus musculus
2.3.1.23 AGPAT8
-
 Mus musculus
2.3.1.23 Alcat1
-
 Mus musculus
2.3.1.23 Lclat1
-
 Mus musculus
2.3.1.23 LPC acyltransferase
-
 Mus musculus
2.3.1.23 LPC acyltransferase
-
 Homo sapiens
2.3.1.23 LPCAT1
-
 Mus musculus
2.3.1.23 LPCAT1
-
 Homo sapiens
2.3.1.23 LPCAT3
-
 Mus musculus
2.3.1.23 LPG acyltransferase
-
 Mus musculus
2.3.1.23 LPGAT
-
 Mus musculus
2.3.1.23 LPLAT
-
 Mus musculus
2.3.1.23 LPLAT
-
 Homo sapiens
2.3.1.23 LYCAT
-
 Mus musculus
2.3.1.23 lysophospholipid acyltransferase
-
 Mus musculus
2.3.1.23 lysophospholipid acyltransferase
-
 Homo sapiens
2.3.1.23 More cf. EC 2.3.1.51 and 2.3.1.62 Mus musculus
2.3.1.23 More cf. EC 2.3.1.62 Mus musculus
2.3.1.23 More cf. EC 2.3.1.62 Homo sapiens
2.3.1.62 acyl-CoA:lysocardiolipin acyltransferase 2
-
 Mus musculus
2.3.1.62 AGPAT8
-
 Mus musculus
2.3.1.62 Alcat1
-
 Mus musculus
2.3.1.62 Lclat1
-
 Mus musculus
2.3.1.62 LPC acyltransferase
-
 Mus musculus
2.3.1.62 LPC acyltransferase
-
 Homo sapiens
2.3.1.62 LPCAT1
-
 Mus musculus
2.3.1.62 LPCAT1
-
 Homo sapiens
2.3.1.62 LPLAT
-
 Mus musculus
2.3.1.62 LPLAT
-
 Homo sapiens
2.3.1.62 LYCAT
-
 Mus musculus
2.3.1.62 lysophospholipid acyltransferase
-
 Mus musculus
2.3.1.62 lysophospholipid acyltransferase
-
 Homo sapiens
2.3.1.62 More cf. EC 2.3.1.23 Mus musculus
2.3.1.62 More cf. EC 2.3.1.23 Homo sapiens
2.3.1.62 More cf. EC 2.3.1.51 and 2.3.1.23 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.23 37
-
 assay at Mus musculus
2.3.1.23 37
-
 assay at Homo sapiens
2.3.1.62 37
-
 assay at Mus musculus
2.3.1.62 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.23 7.4
-
 assay at Mus musculus
2.3.1.23 7.4
-
 assay at Homo sapiens
2.3.1.62 7.4
-
 assay at Mus musculus
2.3.1.62 7.4
-
 assay at Homo sapiens