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Literature summary extracted from

  • Trilisenko, L.V.; Andreeva, N.A.; Eldarov, M.A.; Dumina, M.V.; Kulakovskaya, T.V.
    Polyphosphates and polyphosphatase activity in the yeast Saccharomyces cerevisiae during overexpression of the DDP1 gene (2015), Biochemistry, 80, 1312-1317 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.10 gene DDP1, recombinant overexpression in Saccharomyces cerevisiae strain CRN. The initial strain CRN lacks the endopolyphosphatase PPN1, but has its own protein DDP1, which accounts for the low endopolyphosphatase activity in this strain. The recombinant DDP1 enzyme shows an endopolyphosphatase activity, the endopolyphosphatase activity of the transformant manifests itself both with long-chain polyP208 and with short-chain polyP15. Content of phosphate and polyphosphate in cells of CRN, overview Saccharomyces cerevisiae
3.6.1.52 gene DDP1, recombinant overexpression in Saccharomyces cerevisiae strain CRN. The initial strain CRN lacks the endopolyphosphatase PPN1, but has its own protein DDP1, which accounts for the low endopolyphosphatase activity in this strain. The recombinant DDP1 enzyme shows an endopolyphosphatase activity, the endopolyphosphatase activity of the transformant manifests itself both with long-chain polyP208 and with short-chain polyP15. Content of phosphate and polyphosphate in cells of CRN, overview Saccharomyces cerevisiae
3.6.1.60 gene DDP1, recombinant overexpression in Saccharomyces cerevisiae strain CRN. The initial strain CRN lacks the endopolyphosphatase PPN1, but has its own protein DDP1, which accounts for the low endopolyphosphatase activity in this strain. The recombinant DDP1 enzyme shows an endopolyphosphatase activity, the endopolyphosphatase activity of the transformant manifests itself both with long-chain polyP208 and with short-chain polyP15. Content of phosphate and polyphosphate in cells of CRN, overview Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.10 additional information overexpression of yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) in Saccharomyces cerevisiae leads to significantly increased compared to the parent strain. The content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively Saccharomyces cerevisiae
3.6.1.52 additional information overexpression of yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) in Saccharomyces cerevisiae leads to significantly increased compared to the parent strain. The content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively Saccharomyces cerevisiae
3.6.1.60 additional information overexpression of yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) in Saccharomyces cerevisiae leads to significantly increased compared to the parent strain. The content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.10 ATP inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.10 diphosphate inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.10 additional information ADP has no effect on the endopolyphophatase activity of recombinant DDP1 Saccharomyces cerevisiae
3.6.1.10 phosphate inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.10 Triphosphate inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.52 ATP inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.52 diphosphate inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.52 additional information ADP has no effect on the endopolyphophatase activity of recombinant DDP1 Saccharomyces cerevisiae
3.6.1.52 phosphate inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.52 Triphosphate inhibitory at 2 mM; inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.60 ATP inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.60 diphosphate inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.60 additional information ADP has no effect on the endopolyphophatase activity of recombinant DDP1 Saccharomyces cerevisiae
3.6.1.60 phosphate inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae
3.6.1.60 Triphosphate inhibits the endopolyphophatase of the recombinant enzyme DDP1 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.52 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.10 Mg2+ required, activates Saccharomyces cerevisiae
3.6.1.52 Mg2+ required, activates Saccharomyces cerevisiae
3.6.1.60 Mg2+ required, activates Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.52 diphospho-myo-inositol polyphosphate + H2O Saccharomyces cerevisiae
-
 myo-inositol polyphosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.10 Saccharomyces cerevisiae Q99321 cf. EC 3.6.1.60 and EC 3.6.1.52
-
3.6.1.10 Saccharomyces cerevisiae ATCC 204508 Q99321 cf. EC 3.6.1.60 and EC 3.6.1.52
-
3.6.1.52 Saccharomyces cerevisiae Q99321
-
-
3.6.1.52 Saccharomyces cerevisiae Q99321 cf. EC 3.6.1.60 and EC 3.6.1.10
-
3.6.1.52 Saccharomyces cerevisiae ATCC 204508 Q99321 cf. EC 3.6.1.60 and EC 3.6.1.10
-
3.6.1.60 Saccharomyces cerevisiae Q99321 cf. EC 3.6.1.10 and EC 3.6.1.52
-
3.6.1.60 Saccharomyces cerevisiae ATCC 204508 Q99321 cf. EC 3.6.1.10 and EC 3.6.1.52
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.10 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ?
-
-
3.6.1.10 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ATCC 204508 ?
-
-
3.6.1.10 polyphosphate15 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.6.1.10 polyphosphate15 + H2O
-
 Saccharomyces cerevisiae ATCC 204508 ?
-
 ?
3.6.1.10 polyphosphate208 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.6.1.10 polyphosphate208 + H2O
-
 Saccharomyces cerevisiae ATCC 204508 ?
-
 ?
3.6.1.52 diphosphate + H2O
-
 Saccharomyces cerevisiae 2 phosphate
-
 ?
3.6.1.52 diphosphate + H2O
-
 Saccharomyces cerevisiae ATCC 204508 2 phosphate
-
 ?
3.6.1.52 diphospho-myo-inositol polyphosphate + H2O
-
 Saccharomyces cerevisiae myo-inositol polyphosphate + phosphate
-
 ?
3.6.1.52 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ?
-
-
3.6.1.52 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ATCC 204508 ?
-
-
3.6.1.52 polyP15 + H2O
-
 Saccharomyces cerevisiae polyP14 + phosphate
-
 ?
3.6.1.52 polyP208 + H2O
-
 Saccharomyces cerevisiae polyP207 + phosphate
-
 ?
3.6.1.52 polyphosphate15 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.6.1.52 polyphosphate208 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.6.1.52 triphosphate + H2O
-
 Saccharomyces cerevisiae diphosphate + phosphate
-
 ?
3.6.1.60 additional information endopolyphosphatase activity is analyzed by the decrease in polyphosphate chain length (polyphosphate208 and polyphosphate15) Saccharomyces cerevisiae ?
-
-
3.6.1.60 polyphosphate15 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?
3.6.1.60 polyphosphate208 + H2O
-
 Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.10 endopolyphosphatase
-
 Saccharomyces cerevisiae
3.6.1.10 More see also EC 3.6.1.60 and EC 3.6.1.52 Saccharomyces cerevisiae
3.6.1.52 DDP1
-
 Saccharomyces cerevisiae
3.6.1.52 diphosphoinositol polyphosphate phosphohydrolase
-
 Saccharomyces cerevisiae
3.6.1.52 More see also EC 3.6.1.60 and EC 3.6.1.10 Saccharomyces cerevisiae
3.6.1.60 DDP1
-
 Saccharomyces cerevisiae
3.6.1.60 diphosphoinositol polyphosphate phosphohydrolase
-
 Saccharomyces cerevisiae
3.6.1.60 More see also EC 3.6.1.10 and EC 3.6.1.52 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.6.1.10 30
-
 assay at Saccharomyces cerevisiae
3.6.1.52 30
-
 assay at Saccharomyces cerevisiae
3.6.1.60 30
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.10 7.2
-
 assay at Saccharomyces cerevisiae
3.6.1.52 7.2
-
 assay at Saccharomyces cerevisiae
3.6.1.60 7.2
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
3.6.1.10 malfunction the content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively. The average chain length of salt-soluble and alkali-soluble fractions does not change in the overexpressing strain, and that of acid-soluble polyphosphate increases under phosphate excess. At the initial stage of polyphosphate recovery after phosphorus starvation, the chain length of the acid-soluble fraction in transformed cells is lower compared to the recipient strain. In DDP1 deletion mutant, the level of inositol pyrophosphate is twice higher, while the level of polyphosphate is reduced. The overexpression of DDP1 probably leads to a decrease in the level of diphosphoinositol pentakisphosphate and bis(diphosphoinositol) tetrakisphosphate in the cell. These compounds seem to be involved in the regulation of polyphosphate synthesis and degradation Saccharomyces cerevisiae
3.6.1.10 metabolism diphosphoinositol polyphosphate phosphohydrolase (DDP1, EC 3.6.1.52) is also a diadenosine hexaphosphate hydrolase (AMP-forming) (EC 3.6.1.60) and shows endopolyphosphatase (EC 3.6.1.10) activity. The relationship between inositol pyrophosphate and polyphosphate metabolisms seems to be complicated Saccharomyces cerevisiae
3.6.1.10 physiological function yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) having endopolyphosphatase activity on inorganic polyphosphate metabolism in Saccharomyces cerevisiae. Complex nature of DDP1 involvement in the regulation of polyphosphate content and chain length in yeasts Saccharomyces cerevisiae
3.6.1.52 malfunction the content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively. The average chain length of salt-soluble and alkali-soluble fractions does not change in the overexpressing strain, and that of acid-soluble polyphosphate increases under phosphate excess. At the initial stage of polyphosphate recovery after phosphorus starvation, the chain length of the acid-soluble fraction in transformed cells is lower compared to the recipient strain. In DDP1 deletion mutant, the level of inositol pyrophosphate is twice higher, while the level of polyphosphate is reduced. The overexpression of DDP1 probably leads to a decrease in the level of diphosphoinositol pentakisphosphate and bis(diphosphoinositol) tetrakisphosphate in the cell. These compounds seem to be involved in the regulation of polyphosphate synthesis and degradation Saccharomyces cerevisiae
3.6.1.52 metabolism diphosphoinositol polyphosphate phosphohydrolase (DDP1, EC 3.6.1.52) is also a diadenosine hexaphosphate hydrolase (AMP-forming) (EC 3.6.1.60) and shows endopolyphosphatase (EC 3.6.1.10) activity. The relationship between inositol pyrophosphate and polyphosphate metabolisms seems to be complicated Saccharomyces cerevisiae
3.6.1.52 physiological function yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) having endopolyphosphatase activity on inorganic polyphosphate metabolism in Saccharomyces cerevisiae. Complex nature of DDP1 involvement in the regulation of polyphosphate content and chain length in yeasts Saccharomyces cerevisiae
3.6.1.60 malfunction the content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreases by 9 and 28%, respectively. The average chain length of salt-soluble and alkali-soluble fractions does not change in the overexpressing strain, and that of acid-soluble polyphosphate increases under phosphate excess. At the initial stage of polyphosphate recovery after phosphorus starvation, the chain length of the acid-soluble fraction in transformed cells is lower compared to the recipient strain. In DDP1 deletion mutant, the level of inositol pyrophosphate is twice higher, while the level of polyphosphate is reduced. The overexpression of DDP1 probably leads to a decrease in the level of diphosphoinositol pentakisphosphate and bis(diphosphoinositol) tetrakisphosphate in the cell. These compounds seem to be involved in the regulation of polyphosphate synthesis and degradation Saccharomyces cerevisiae
3.6.1.60 metabolism diphosphoinositol polyphosphate phosphohydrolase (DDP1, EC 3.6.1.52) is also a diadenosine hexaphosphate hydrolase (AMP-forming) (EC 3.6.1.60) and shows endopolyphosphatase (EC 3.6.1.10) activity. The relationship between inositol pyrophosphate and polyphosphate metabolisms seems to be complicated Saccharomyces cerevisiae
3.6.1.60 physiological function yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) having endopolyphosphatase activity on inorganic polyphosphate metabolism in Saccharomyces cerevisiae. Complex nature of DDP1 involvement in the regulation of polyphosphate content and chain length in yeasts Saccharomyces cerevisiae