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Literature summary extracted from

  • Robbins, T.; Kapilivsky, J.; Cane, D.E.; Khosla, C.
    Roles of conserved active site residues in the ketosynthase domain of an assembly line polyketide synthase (2016), Biochemistry, 55, 4476-4484 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.94 expression in Escherichia coli Saccharopolyspora erythraea

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.94 C211A mutation at the ketosynthase domain active site, exhibits no turnover activity, but is still a competent methylmalonyl-ACP decarboxylase Saccharopolyspora erythraea
2.3.1.94 H346A mutation at the ketosynthase domain, mutant exhibits reduced rates of both chain translocation and chain elongation, with a greater effect on the latter half-reaction Saccharopolyspora erythraea
2.3.1.94 H384A mutation at the ketosynthase domain, residue contributes to methylmalonyl-ACP decarboxylation Saccharopolyspora erythraea
2.3.1.94 H457A activity similar to wild-type Saccharopolyspora erythraea
2.3.1.94 K379A mutation at the ketosynthase domain, residue promotes C-C bond formation Saccharopolyspora erythraea
2.3.1.94 N381A increase in activity Saccharopolyspora erythraea
2.3.1.94 N455L activity similar to wild-type Saccharopolyspora erythraea
2.3.1.94 Q386E about 25% of wild-type activity Saccharopolyspora erythraea
2.3.1.94 S315A mutation at the ketosynthase domain, residue plays a role in coupling decarboxylation to C-C bond formation Saccharopolyspora erythraea
2.3.1.94 T209A increase in activity Saccharopolyspora erythraea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.94 Saccharopolyspora erythraea Q03131 and Q03132 and Q03133 Q03131 i.e. modules 1 and 2, Q03132 i.e. modules 3 and 4, Q03133 i.e. modules 5 and 6
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.94 propanoyl-CoA + (2S)-methylmalonyl-CoA + NADPH
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 Saccharopolyspora erythraea (3R,4S,5R,6R)-6-ethyl-4-hydroxy-3,5-dimethyloxan-2-one + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.94 eryA
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 Saccharopolyspora erythraea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.94 0.0067
-
 propanoyl-CoA mutant H384A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.028
-
 propanoyl-CoA mutant Q386E, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.032
-
 propanoyl-CoA mutant H457A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.038
-
 propanoyl-CoA mutant N381A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.038
-
 propanoyl-CoA mutant N455L, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.04
-
 propanoyl-CoA mutant S315A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.042
-
 propanoyl-CoA wild-type, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea
2.3.1.94 0.048
-
 propanoyl-CoA mutant T209A, pH 7.2, temperature not specified in the publication Saccharopolyspora erythraea