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Literature summary extracted from

  • Sautner, V.; Friedrich, M.M.; Lehwess-Litzmann, A.; Tittmann, K.
    Converting transaldolase into aldolase through swapping of the multifunctional acid-base catalyst common and divergent catalytic principles in F6P aldolase and transaldolase (2015), Biochemistry, 54, 4475-4486 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.2.1.2 mutant enzyme E60Q/F132Y, hanging drop vapor diffusion method, using 200 mM ammonium acetate (pH 4.4), 10% (w/v) PEG 6000, and 25% (v/v) glycerol Thermoplasma acidophilum

Protein Variants

EC Number Protein Variants Comment Organism
2.2.1.2 E60Q/F132Y the mutant enzyme has no transaldolase activity and shows D-fructose 6-phosphate aldolase activity Thermoplasma acidophilum
2.2.1.2 F132Y the mutant enzyme has no transaldolase activity and shows D-fructose 6-phosphate aldolase activity Thermoplasma acidophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.2 2.3
-
 D-fructose 6-phosphate wild type enzyme, at pH 7.5 and 30°C Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate Thermoplasma acidophilum
-
 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.2 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate Thermoplasma acidophilum
-
 D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.2 Thermoplasma acidophilum Q9HKI3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.2 D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 Thermoplasma acidophilum sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 r
2.2.1.2 additional information the enzyme exhibits a very small yet detectable D-fructose 6-phosphate aldolase activity Thermoplasma acidophilum ?
-
-
2.2.1.2 sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
 Thermoplasma acidophilum D-erythrose 4-phosphate + D-fructose 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.2.1.2 TAL
-
 Thermoplasma acidophilum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.2.1.2 0.24
-
 D-fructose 6-phosphate mutant enzyme F132Y, at pH 7.5 and 30°C Thermoplasma acidophilum
2.2.1.2 0.62
-
 D-fructose 6-phosphate mutant enzyme E60Q/F132Y, at pH 7.5 and 30°C Thermoplasma acidophilum
2.2.1.2 13.86
-
 D-fructose 6-phosphate wild type enzyme, at pH 7.5 and 30°C Thermoplasma acidophilum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2.1.2 6.0261
-
 D-fructose 6-phosphate wild type enzyme, at pH 7.5 and 30°C Thermoplasma acidophilum