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Literature summary extracted from

  • Brockhausen, I.; Nair, D.G.; Chen, M.; Yang, X.; Allingham, J.S.; Szarek, W.A.; Anastassiades, T.
    Human acetyl-CoA glucosamine-6-phosphate N-acetyltransferase 1 has a relaxed donor specificity and transfers acyl groups up to four carbons in length (2016), Biochem. Cell Biol., 94, 197-204 .
    View publication on PubMed

Application

EC Number Application Comment Organism
2.3.1.4 analysis development of a fluorescence assay where reactive thiols of CoA, generated from the acyl transfer reaction, are monitored using monobromobimane Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.4 C157A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 L138A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 1 Homo sapiens
2.3.1.4 L155A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 L155A/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 L155G mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.9 Homo sapiens
2.3.1.4 L155G/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.85 Homo sapiens
2.3.1.4 L158A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 L158A/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 L158G mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 L158G/N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 N161A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 P159A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity Homo sapiens
2.3.1.4 Y165A mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.9 Homo sapiens
2.3.1.4 Y165T mutant shows activities of both acetyl- and butyryltransfer, in the range of the wild type enzyme activity. Ratio of butanoyl-to-acetyltransferase activity is increased to 0.9 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.4 additional information not inhibitory: 1,22-bis(3-methyl-1H-imidazolium-1-yl)docosane dimesylate, N-butanoylglucosaminex02beta-S-2-naphthyl Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.4 0.13
-
propionyl-CoA pH 7.6, 37°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.4 Homo sapiens Q96EK6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.4 acetyl-CoA + D-glucosamine 6-phosphate
-
Homo sapiens CoA + N-acetyl-D-glucosamine 6-phosphate
-
?
2.3.1.4 butanoyl-CoA + D-glucosamine 6-phosphate
-
Homo sapiens CoA + N-butanoyl-D-glucosamine 6-phosphate
-
?
2.3.1.4 isobutanoyl-CoA + D-glucosamine 6-phosphate
-
Homo sapiens CoA + N-isobutanoyl-D-glucosamine 6-phosphate
-
?
2.3.1.4 additional information ratio of butanoyl-to-acetyltransferase activity for wild-type: 0.6. No substrates: isovaleryl-CoA and decanoyl-CoA Homo sapiens ?
-
-
2.3.1.4 propionyl-CoA + D-glucosamine 6-phosphate
-
Homo sapiens CoA + N-propionyl-D-glucosamine 6-phosphate
-
?