EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.1 | gene TcasGA2_TC004566, real-time PCR expression analysis | Tribolium castaneum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.1 | additional information | knockdown of Tc-SPPase in the genome of Tribolium castaneum via pRNAi. Injection of Tc-sPPase dsRNA completely inhibits its expression when compared to the control, confirming that Tc-sPPase transcription is largely reduced | Tribolium castaneum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.1 | mitochondrion | - |
Tribolium castaneum | 5739 | - |
3.6.1.1 | soluble | - |
Tribolium castaneum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.1 | Mg2+ | required | Tribolium castaneum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | Tribolium castaneum | - |
2 phosphate | - |
? | |
3.6.1.1 | triphosphate + H2O | Tribolium castaneum | - |
3 phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.1 | Tribolium castaneum | D6WB60 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | - |
Tribolium castaneum | 2 phosphate | - |
? | |
3.6.1.1 | triphosphate + H2O | - |
Tribolium castaneum | 3 phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.1 | inorganic pyrophosphatase | - |
Tribolium castaneum |
3.6.1.1 | inorganic pyrophosphatase-like protein | UniProt | Tribolium castaneum |
3.6.1.1 | PPase | - |
Tribolium castaneum |
3.6.1.1 | soluble inorganic pyrophosphatase | - |
Tribolium castaneum |
3.6.1.1 | Tc-sPPase | - |
Tribolium castaneum |
3.6.1.1 | TC004566 | - |
Tribolium castaneum |
3.6.1.1 | TcasGA2_TC004566 | locus name | Tribolium castaneum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.6.1.1 | malfunction | tripolyphosphate is able to increase the F-ATPase activity in wild-type and Tc-sPPase RNAi beetles. sPPase gene knock-down influences polyP metabolism in mitochondria, mainly tripolyphosphate metabolism | Tribolium castaneum |
3.6.1.1 | metabolism | inorganic pyrophosphatase modulates polyphosphate metabolism in mitochondria and affects the link between mitochondrial activity and polyphosphate metabolism in Tibolium castaneum. Mitochondrial respiration modulates exopolyphosphatase activity (EC 3.6.1.11) only in wild-type beetles. The soluble form has a greater affinity for polyP3, and the membrane form has a greater affinity for polyP15, with only the soluble PPX activity being affected by Tc-sPPase RNAi | Tribolium castaneum |
3.6.1.1 | physiological function | presence of two exopolyphosphatase isoforms in mitochondria. Tribolium castaneum mitochondrial polyP levels decrease after injection with soluble diphosphatase (Tc-sPPase) dsRNA, while the membrane exopolyphosphate activity (EC 3.6.1.11) increases | Tribolium castaneum |