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Literature summary extracted from

  • Marcella, A.M.; Barb, A.W.
    The R117A variant of the Escherichia coli transacylase FabD synthesizes novel acyl-(acyl carrier proteins) (2017), Appl. Microbiol. Biotechnol., 101, 8431-8441 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.39 R117A the variant synthesizes acetyl-[acyl carrier protein], succinyl-[acyl carrier protein], isobutyryl-[acyl carrier protein], 2-butenoyl-[acyl carrier protein], and beta-hydroxybutyryl-[acyl carrier protein] among others from holo-acyl carrier protein and the corresponding acyl-CoAs Escherichia coli
2.3.1.39 R117A unlike the wild-type enzyme that is highly specific for malonyl-CoA to produce malonyl-[acyl-carrier protein] (ACP), the R117A variant synthesizes acetyl-ACP, succinyl-ACP, isobutyryl-ACP, 2-butenoyl-ACP, and beta-hydroxybutyryl-ACP among others from holo-ACP and the corresponding acyl-CoAs with specific activities from 3.7 to 120 nmol/min*mg Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.011
-
an [acyl-carrier protein] mutant enzyme R117A, with methylmalonyl-CoA as cosubstrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.02
-
an [acyl-carrier protein] wild type enzyme, with methylmalonyl-CoA as cosubstrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.03
-
an [acyl-carrier protein] mutant enzyme R117A, with acetoacetyl-CoA as cosubstrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.03
-
an [acyl-carrier protein] mutant enzyme R117A, with malonyl-CoA as cosubstrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.04
-
an [acyl-carrier protein] mutant enzyme R117A, with acetyl-CoA as cosubstrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.04
-
an [acyl-carrier protein] wild type enzyme, with malonyl-CoA as cosubstrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.06
-
malonyl-CoA wild type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.06
-
malonyl-CoA wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.08
-
malonyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.11
-
acetoacetyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.2
-
acetyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.33
-
methylmalonyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.58
-
methylmalonyl-CoA wild type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.58
-
methylmalonyl-CoA wild-type enzyme, pH and temperature not specified in the publication Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] Escherichia coli
-
CoA + a malonyl-[acyl-carrier protein]
-
?
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] Escherichia coli the wild type enzyme is highly specific for malonyl-CoA CoA + a malonyl-[acyl-carrier protein]
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.39 0.001
-
mutant enzyme R117A with isovaleryl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.0016
-
substrate: acetoacetyl-CoA, wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.0016
-
wild type enzyme with acetoacetyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.0037
-
mutant enzyme R117A with isobutyryl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.005
-
mutant enzyme R117A with 2-hydroxybutyryl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.0054
-
mutant enzyme R117A with 2-butenoyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.011
-
mutant enzyme R117A with butyryl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.016
-
mutant enzyme R117A with succinyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.052
-
mutant enzyme R117A with propionyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.06
-
mutant enzyme R117A with acetoacetyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.06
-
substrate: acetoacetyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.12
-
mutant enzyme R117A with acetyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.12
-
substrate: acetyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 3.2
-
mutant enzyme R117A with methylmalonyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 3.2
-
substrate: methylmalonyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 13
-
mutant enzyme R117A with malonyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 13
-
substrate: malonyl-CoA, mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 270
-
substrate: methylmalonyl-CoA, wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 270
-
wild type enzyme with methylmalonyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 4100
-
substrate: malonyl-CoA, wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 4100
-
wild type enzyme with malonyl-CoA as substrate, pH and temperature not specified in the publication Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 2-butenoyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + 2-butenoyl-[acyl carrier protein]
-
?
2.3.1.39 2-hydroxybutyryl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + 2-hydroxybutyryl-[acyl carrier protein]
-
?
2.3.1.39 acetoacetyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by the wild type enzyme (very weak activity) and mutant enzyme R117A Escherichia coli CoA + acetoacetyl-[acyl carrier protein]
-
?
2.3.1.39 acetoacetyl-CoA + an [acyl-carrier protein]
-
Escherichia coli CoA + an acetoacetyl-[acyl-carrier protein]
-
?
2.3.1.39 acetyl-CoA + an [acyl-carrier protein] activity with mutant enzyme R117A, no activity with wild-type enzyme Escherichia coli CoA + an acetyl-[acyl-carrier protein]
-
?
2.3.1.39 acetyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + acetyl-[acyl carrier protein]
-
?
2.3.1.39 butyryl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + butyryl-[acyl carrier protein]
-
?
2.3.1.39 isobutyryl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + isobutyryl-[acyl carrier protein]
-
?
2.3.1.39 isovaleryl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + isovaleryl-[acyl carrier protein]
-
?
2.3.1.39 malonyl-CoA + an [acyl-carrier protein]
-
Escherichia coli CoA + a malonyl-[acyl-carrier protein]
-
?
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] the wild type enzyme is highly specific for malonyl-CoA Escherichia coli CoA + a malonyl-[acyl-carrier protein]
-
?
2.3.1.39 malonyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by the wild type enzyme and mutant enzyme R117A Escherichia coli CoA + a malonyl-[acyl-carrier protein]
-
?
2.3.1.39 methylmalonyl-CoA + an [acyl-carrier protein]
-
Escherichia coli CoA + methylmalonyl-[acyl-carrier protein]
-
?
2.3.1.39 methylmalonyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by the wild type enzyme (low activity) and mutant enzyme R117A Escherichia coli CoA + methylmalonyl-[acyl carrier protein]
-
?
2.3.1.39 additional information the wild type enzyme shows no activity with acetyl-CoA, propionyl-CoA, succinyl-CoA, butyryl-CoA, 2-butenoyl-CoA, 2-hydroxybutyryl-CoA, isobutyryl-CoA, and isovaleryl-CoA Escherichia coli ?
-
-
2.3.1.39 propionyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + propionyl-[acyl carrier protein]
-
?
2.3.1.39 succinyl-CoA + an [acyl-carrier protein] the reaction is catalyzed by mutant enzyme R117A Escherichia coli CoA + succinyl-[acyl carrier protein]
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 FabD
-
Escherichia coli
2.3.1.39 malonyl-CoA/holo-ACP transacylase
-
Escherichia coli
2.3.1.39 malonyl-coenzyme A (CoA)/holo-ACP transacylase
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.39 0.029
-
acetyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 0.041
-
acetoacetyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 5
-
methylmalonyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 8.5
-
malonyl-CoA mutant enzyme R117A, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 1000
-
methylmalonyl-CoA wild type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 1000
-
methylmalonyl-CoA wild-type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 2100
-
malonyl-CoA wild type enzyme, pH and temperature not specified in the publication Escherichia coli
2.3.1.39 2100
-
malonyl-CoA wild-type enzyme, pH and temperature not specified in the publication Escherichia coli